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INSR gene variation is associated with decreased insulin sensitivity in Iraqi women with PCOs.

T Mutib M, B Hamdan F, R Al-Salihi A - Iran J Reprod Med (2014)

Bottom Line: To examine whether the exon 17 of INSR gene contributes to genetic susceptibility to PCOS in Iraqi women and its effects on glucose tolerance test and lipid profile.Clinical, anthropometric and biochemical parameters were also estimated.Its association with indices of insulin resistance and dyslipidemia were also noticed.

View Article: PubMed Central - PubMed

Affiliation: The High Institute of Infertility Diagnosis and Assisted Reproductive Technology, Al-Nahrain University, Baghdad, Iraq.

ABSTRACT

Background: Polycystic ovarian syndrome (PCOS) is a complex, heterogeneous disorder of uncertain etiology with strong genetic background. Insulin resistance is present in the majority of PCOS cases with linkage and association between single nucleotide polymorphisms of insulin receptor (INSR) gene and PCOS.

Objective: To examine whether the exon 17 of INSR gene contributes to genetic susceptibility to PCOS in Iraqi women and its effects on glucose tolerance test and lipid profile.

Materials and methods: Sixty-five healthy Iraqi women and eighty-four infertile women with PCOS, divided into two subgroups depending on the BMI were studied. Restriction fragment length polymorphism (RFLP-PCR) analysis was performed to determine the genotypes for the His 1058 C/T polymorphism at the tyrosine kinase domain in the INSR gene. Clinical, anthropometric and biochemical parameters were also estimated.

Results: The C/T polymorphism at His 1058 in exon 17 of INSR was associated with PCOS (obese and non-obese). CC genotype frequency was higher in PCOS patients whereas TT genotype was higher in control women. Those with CC genotype had higher BMI, GTT and lipid profile than those with TT genotype.

Conclusion: An association of C/T polymorphism at His1058 of INSR with PCOS in Iraqi women was observed. Its association with indices of insulin resistance and dyslipidemia were also noticed.

No MeSH data available.


Related in: MedlinePlus

Restriction fragment length polymorphism analysis of the C/T polymorphism of exon 17 in the INSR gene. Agarose gel (2%) electrophoresis after PmlI digestion of the PCR
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Figure 1: Restriction fragment length polymorphism analysis of the C/T polymorphism of exon 17 in the INSR gene. Agarose gel (2%) electrophoresis after PmlI digestion of the PCR

Mentions: Digested DNA fragments were electrophoresed on a 2% agarose gel containing ethidium bromide and visualized by UV trans-illuminator spectroline (USA). Hence, a single 317-bp band indicates homozygosity for the TT genotype. The presence of two fragments, 274-bp and 43-bp bands, indicates homozygosity for the CC genotype. The presence of three fragments, 317-, 274-, and 43-bp bands, indicates heterozygosity for the CT genotype (Figure 1).


INSR gene variation is associated with decreased insulin sensitivity in Iraqi women with PCOs.

T Mutib M, B Hamdan F, R Al-Salihi A - Iran J Reprod Med (2014)

Restriction fragment length polymorphism analysis of the C/T polymorphism of exon 17 in the INSR gene. Agarose gel (2%) electrophoresis after PmlI digestion of the PCR
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126255&req=5

Figure 1: Restriction fragment length polymorphism analysis of the C/T polymorphism of exon 17 in the INSR gene. Agarose gel (2%) electrophoresis after PmlI digestion of the PCR
Mentions: Digested DNA fragments were electrophoresed on a 2% agarose gel containing ethidium bromide and visualized by UV trans-illuminator spectroline (USA). Hence, a single 317-bp band indicates homozygosity for the TT genotype. The presence of two fragments, 274-bp and 43-bp bands, indicates homozygosity for the CC genotype. The presence of three fragments, 317-, 274-, and 43-bp bands, indicates heterozygosity for the CT genotype (Figure 1).

Bottom Line: To examine whether the exon 17 of INSR gene contributes to genetic susceptibility to PCOS in Iraqi women and its effects on glucose tolerance test and lipid profile.Clinical, anthropometric and biochemical parameters were also estimated.Its association with indices of insulin resistance and dyslipidemia were also noticed.

View Article: PubMed Central - PubMed

Affiliation: The High Institute of Infertility Diagnosis and Assisted Reproductive Technology, Al-Nahrain University, Baghdad, Iraq.

ABSTRACT

Background: Polycystic ovarian syndrome (PCOS) is a complex, heterogeneous disorder of uncertain etiology with strong genetic background. Insulin resistance is present in the majority of PCOS cases with linkage and association between single nucleotide polymorphisms of insulin receptor (INSR) gene and PCOS.

Objective: To examine whether the exon 17 of INSR gene contributes to genetic susceptibility to PCOS in Iraqi women and its effects on glucose tolerance test and lipid profile.

Materials and methods: Sixty-five healthy Iraqi women and eighty-four infertile women with PCOS, divided into two subgroups depending on the BMI were studied. Restriction fragment length polymorphism (RFLP-PCR) analysis was performed to determine the genotypes for the His 1058 C/T polymorphism at the tyrosine kinase domain in the INSR gene. Clinical, anthropometric and biochemical parameters were also estimated.

Results: The C/T polymorphism at His 1058 in exon 17 of INSR was associated with PCOS (obese and non-obese). CC genotype frequency was higher in PCOS patients whereas TT genotype was higher in control women. Those with CC genotype had higher BMI, GTT and lipid profile than those with TT genotype.

Conclusion: An association of C/T polymorphism at His1058 of INSR with PCOS in Iraqi women was observed. Its association with indices of insulin resistance and dyslipidemia were also noticed.

No MeSH data available.


Related in: MedlinePlus