Limits...
Evaluating the effects of Escanbil (Calligonum) extract on the expression level of Catsper gene variants and sperm motility in aging male mice.

Askari Jahromi M, Movahedin M, Mazaheri Z, Amanlu M, Mowla SJ, Batooli H - Iran J Reprod Med (2014)

Bottom Line: Sperm parameters were analyzed.Our results showed that after Escanbil (Calligonum) treatment (30 mg/kg), the sperm parameters were improved in experimental group (p<0.05).This herbal extract can be used as an antioxidant component for clinical usages.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

ABSTRACT

Background: Catsper proteins are responsible for entering Ca(2+) to the cell and play an important role in sperm motility and male fertility. Antioxidants are vital for sperm motility too. Escanbil (Calligonum) extract possess some of the important antioxidant like Catechin and Quercetin.

Objective: Here we investigated the effects of Escanbil (Calligonum) extract on the sperm parameters and the expressing of Catsper gene in aging male mice.

Materials and methods: In this animal study, firstly, dose response was performed by using these three doses of Escanbil (Calligonum) (10, 30 and 50 mg/kg). 5 mice in each group were considered and Intra Peritoneal injection was done for 5 weeks. the sperm parameters analyzed and dUTP nick end labeling (TUNEL )staining was done. 30 mg/kg dose was considered as optimum dose. Secondly: fifteen aging male mice (11-13 months) were divided into three groups: control, sham and experiment. The experiments were injected Intra peritonealy with Escanbil (Calligonum) extract (30mg/kg) weekly for up to 5 weeks. The sham group was injected Intra Peritoneal (DMSO). Sperm parameters were analyzed. Expression of Catsper genes was analyzed by Real time PCR.

Results: Our results showed that after Escanbil (Calligonum) treatment (30 mg/kg), the sperm parameters were improved in experimental group (p<0.05). Our data showed that there was a statistical significance difference between the expressions of Catsper 2, 4 in aging experiment group comparison with aging control group (p<0.05).

Conclusion: We investigated that the Escanbil (Calligonum) extract (30 mg/kg) can improve sperm parameters and change the expression of Catsper genes in aging male mice. This herbal extract can be used as an antioxidant component for clinical usages.

No MeSH data available.


Related in: MedlinePlus

TUNEL staining indicated decrease of apoptosis of spermatogenic cells in 10 (A), 30 (B), 50 (C) mg/kg, aging control (D), Positive control (E) and Negative control (F). (The Original magnification ×400). The apoptosis rates of spermatogenic cells in (30mg/kg) testes were lower than those of 10 mg/kg and 50 mg/kg and aging control groups
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4126249&req=5

Figure 1: TUNEL staining indicated decrease of apoptosis of spermatogenic cells in 10 (A), 30 (B), 50 (C) mg/kg, aging control (D), Positive control (E) and Negative control (F). (The Original magnification ×400). The apoptosis rates of spermatogenic cells in (30mg/kg) testes were lower than those of 10 mg/kg and 50 mg/kg and aging control groups

Mentions: The results also indicate a decrease in apoptosis cells in the group treated with 30 mg/kg of Escanbil (Calligonum) extract compared to other groups (p<0.05) (Figure 1, Table IV). Our Data analysis showed a significant increase in gene expression Catsper 2, 4 in the aged experimental group compared to the aged control group (p<0.05). Gene expression in the younger group than the older group was significantly increased (p<0.05) (Figure 2).


Evaluating the effects of Escanbil (Calligonum) extract on the expression level of Catsper gene variants and sperm motility in aging male mice.

Askari Jahromi M, Movahedin M, Mazaheri Z, Amanlu M, Mowla SJ, Batooli H - Iran J Reprod Med (2014)

TUNEL staining indicated decrease of apoptosis of spermatogenic cells in 10 (A), 30 (B), 50 (C) mg/kg, aging control (D), Positive control (E) and Negative control (F). (The Original magnification ×400). The apoptosis rates of spermatogenic cells in (30mg/kg) testes were lower than those of 10 mg/kg and 50 mg/kg and aging control groups
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4126249&req=5

Figure 1: TUNEL staining indicated decrease of apoptosis of spermatogenic cells in 10 (A), 30 (B), 50 (C) mg/kg, aging control (D), Positive control (E) and Negative control (F). (The Original magnification ×400). The apoptosis rates of spermatogenic cells in (30mg/kg) testes were lower than those of 10 mg/kg and 50 mg/kg and aging control groups
Mentions: The results also indicate a decrease in apoptosis cells in the group treated with 30 mg/kg of Escanbil (Calligonum) extract compared to other groups (p<0.05) (Figure 1, Table IV). Our Data analysis showed a significant increase in gene expression Catsper 2, 4 in the aged experimental group compared to the aged control group (p<0.05). Gene expression in the younger group than the older group was significantly increased (p<0.05) (Figure 2).

Bottom Line: Sperm parameters were analyzed.Our results showed that after Escanbil (Calligonum) treatment (30 mg/kg), the sperm parameters were improved in experimental group (p<0.05).This herbal extract can be used as an antioxidant component for clinical usages.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

ABSTRACT

Background: Catsper proteins are responsible for entering Ca(2+) to the cell and play an important role in sperm motility and male fertility. Antioxidants are vital for sperm motility too. Escanbil (Calligonum) extract possess some of the important antioxidant like Catechin and Quercetin.

Objective: Here we investigated the effects of Escanbil (Calligonum) extract on the sperm parameters and the expressing of Catsper gene in aging male mice.

Materials and methods: In this animal study, firstly, dose response was performed by using these three doses of Escanbil (Calligonum) (10, 30 and 50 mg/kg). 5 mice in each group were considered and Intra Peritoneal injection was done for 5 weeks. the sperm parameters analyzed and dUTP nick end labeling (TUNEL )staining was done. 30 mg/kg dose was considered as optimum dose. Secondly: fifteen aging male mice (11-13 months) were divided into three groups: control, sham and experiment. The experiments were injected Intra peritonealy with Escanbil (Calligonum) extract (30mg/kg) weekly for up to 5 weeks. The sham group was injected Intra Peritoneal (DMSO). Sperm parameters were analyzed. Expression of Catsper genes was analyzed by Real time PCR.

Results: Our results showed that after Escanbil (Calligonum) treatment (30 mg/kg), the sperm parameters were improved in experimental group (p<0.05). Our data showed that there was a statistical significance difference between the expressions of Catsper 2, 4 in aging experiment group comparison with aging control group (p<0.05).

Conclusion: We investigated that the Escanbil (Calligonum) extract (30 mg/kg) can improve sperm parameters and change the expression of Catsper genes in aging male mice. This herbal extract can be used as an antioxidant component for clinical usages.

No MeSH data available.


Related in: MedlinePlus