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High-Fat-Diet-Induced Weight Gain Ameliorates Bone Loss without Exacerbating AβPP Processing and Cognition in Female APP/PS1 Mice.

Peng Y, Liu J, Tang Y, Liu J, Han T, Han S, Li H, Hou C, Liu J, Long J - Front Cell Neurosci (2014)

Bottom Line: Given that there is no evidence that being overweight is associated with AD-type cognitive dysfunction, we hypothesized that moderate weight gain might have a protective effect on the bone loss in AD without exacerbating cognitive dysfunction.The bone mineral density, microarchitecture, and biomechanical properties of the femurs were then evaluated.These results suggest that a body weight gain induced by the HFD feeding regimen significantly improved bone mass in female APP/PS1 mice with no detriments to exploration ability and spatial memory, most likely via the action of elevated circulating leptin.

View Article: PubMed Central - PubMed

Affiliation: Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology and Frontier Institute of Science and Technology, Xi'an Jiaotong University , Xi'an , China.

ABSTRACT
Osteoporosis is negatively correlated with body mass, whereas both osteoporosis and weight loss occur at higher incidence during the progression of Alzheimer's disease (AD) than the age-matched non-dementia individuals. Given that there is no evidence that being overweight is associated with AD-type cognitive dysfunction, we hypothesized that moderate weight gain might have a protective effect on the bone loss in AD without exacerbating cognitive dysfunction. In this study, feeding a high-fat diet (HFD, 45% calorie from fat) to female APP/PS1 transgenic mice, an AD animal model, induced weight gain. The bone mineral density, microarchitecture, and biomechanical properties of the femurs were then evaluated. The results showed that the middle-aged female APP/PS1 transgenic mice were susceptible to osteoporosis of the femoral bones and that weight gain significantly enhanced bone mass and mechanical properties. Notably, HFD was not detrimental to brain insulin signaling and AβPP processing, as well as to exploration ability and working, learning, and memory performance of the transgenic mice measured by T maze and Morris water maze, compared with the mice fed a normal-fat diet (10% calorie from fat). In addition, the circulating levels of leptin but not estradiol were remarkably elevated in HFD-treated mice. These results suggest that a body weight gain induced by the HFD feeding regimen significantly improved bone mass in female APP/PS1 mice with no detriments to exploration ability and spatial memory, most likely via the action of elevated circulating leptin.

No MeSH data available.


Related in: MedlinePlus

HFD improved microarchitecture of femur trabecular bone in APP/PS1 mice. The volumetric BMD [vBMD (A)], tissue BMD [tBMD (B)], and trabecular thickness [Tb.Th (F)] were not significantly altered in C57BL/6 mice and APP/PS1 mice; however, these parameters significantly increased after HFD. Bone volume fraction [BV/TV (D)] was lower in APP/PS1 mice and recovered after HFD feeding. No significant differences in BMC (C) or trabecular number [Tb. N (E)] were found among the three groups. The trabecular separation [Tb.Sp (G)] was not significantly altered in C57BL/6 mice or APP/PS1 mice, and it was reduced after HFD. Micrographs showed the microarchitecture of femoral trabecule (H) with arrows indicating the representative part. Data were means ± SEM. n = 7 for C57BL/6 mice, n = 8 for APP/PS1 mice, and n = 8 for APP/PS1 + HFD mice. The results were analyzed by one-way ANOVA, followed by Newman–Keuls post hoc test. *p < 0.05; **p < 0.01.
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Figure 6: HFD improved microarchitecture of femur trabecular bone in APP/PS1 mice. The volumetric BMD [vBMD (A)], tissue BMD [tBMD (B)], and trabecular thickness [Tb.Th (F)] were not significantly altered in C57BL/6 mice and APP/PS1 mice; however, these parameters significantly increased after HFD. Bone volume fraction [BV/TV (D)] was lower in APP/PS1 mice and recovered after HFD feeding. No significant differences in BMC (C) or trabecular number [Tb. N (E)] were found among the three groups. The trabecular separation [Tb.Sp (G)] was not significantly altered in C57BL/6 mice or APP/PS1 mice, and it was reduced after HFD. Micrographs showed the microarchitecture of femoral trabecule (H) with arrows indicating the representative part. Data were means ± SEM. n = 7 for C57BL/6 mice, n = 8 for APP/PS1 mice, and n = 8 for APP/PS1 + HFD mice. The results were analyzed by one-way ANOVA, followed by Newman–Keuls post hoc test. *p < 0.05; **p < 0.01.

Mentions: The femoral trabecular bone mass was also evaluated by micro-CT (Figures 6A–H). We found that the vBMD, tBMD, and trabecular thickness (Tb.Th) were the same in APP/PS1 mice and C57BL/6 mice, but these parameters were all significantly increased by the HFD feeding (Figures 6A,B,F,H). The trabecular separation (Tb.Sp) was similar in the APP/PS1 mice compared with the C57BL/6 mice but was reduced in mice on the HFD (Figure 6G). No significant differences in the BMC and trabecular number (Tb.N) were found among all groups (Figures 6C,E). The bone volume fraction (BV/TV) was lower in APP/PS1 mice than in the C57BL/6 mice and was corrected by the HFD feeding (Figure 6D).


High-Fat-Diet-Induced Weight Gain Ameliorates Bone Loss without Exacerbating AβPP Processing and Cognition in Female APP/PS1 Mice.

Peng Y, Liu J, Tang Y, Liu J, Han T, Han S, Li H, Hou C, Liu J, Long J - Front Cell Neurosci (2014)

HFD improved microarchitecture of femur trabecular bone in APP/PS1 mice. The volumetric BMD [vBMD (A)], tissue BMD [tBMD (B)], and trabecular thickness [Tb.Th (F)] were not significantly altered in C57BL/6 mice and APP/PS1 mice; however, these parameters significantly increased after HFD. Bone volume fraction [BV/TV (D)] was lower in APP/PS1 mice and recovered after HFD feeding. No significant differences in BMC (C) or trabecular number [Tb. N (E)] were found among the three groups. The trabecular separation [Tb.Sp (G)] was not significantly altered in C57BL/6 mice or APP/PS1 mice, and it was reduced after HFD. Micrographs showed the microarchitecture of femoral trabecule (H) with arrows indicating the representative part. Data were means ± SEM. n = 7 for C57BL/6 mice, n = 8 for APP/PS1 mice, and n = 8 for APP/PS1 + HFD mice. The results were analyzed by one-way ANOVA, followed by Newman–Keuls post hoc test. *p < 0.05; **p < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
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Figure 6: HFD improved microarchitecture of femur trabecular bone in APP/PS1 mice. The volumetric BMD [vBMD (A)], tissue BMD [tBMD (B)], and trabecular thickness [Tb.Th (F)] were not significantly altered in C57BL/6 mice and APP/PS1 mice; however, these parameters significantly increased after HFD. Bone volume fraction [BV/TV (D)] was lower in APP/PS1 mice and recovered after HFD feeding. No significant differences in BMC (C) or trabecular number [Tb. N (E)] were found among the three groups. The trabecular separation [Tb.Sp (G)] was not significantly altered in C57BL/6 mice or APP/PS1 mice, and it was reduced after HFD. Micrographs showed the microarchitecture of femoral trabecule (H) with arrows indicating the representative part. Data were means ± SEM. n = 7 for C57BL/6 mice, n = 8 for APP/PS1 mice, and n = 8 for APP/PS1 + HFD mice. The results were analyzed by one-way ANOVA, followed by Newman–Keuls post hoc test. *p < 0.05; **p < 0.01.
Mentions: The femoral trabecular bone mass was also evaluated by micro-CT (Figures 6A–H). We found that the vBMD, tBMD, and trabecular thickness (Tb.Th) were the same in APP/PS1 mice and C57BL/6 mice, but these parameters were all significantly increased by the HFD feeding (Figures 6A,B,F,H). The trabecular separation (Tb.Sp) was similar in the APP/PS1 mice compared with the C57BL/6 mice but was reduced in mice on the HFD (Figure 6G). No significant differences in the BMC and trabecular number (Tb.N) were found among all groups (Figures 6C,E). The bone volume fraction (BV/TV) was lower in APP/PS1 mice than in the C57BL/6 mice and was corrected by the HFD feeding (Figure 6D).

Bottom Line: Given that there is no evidence that being overweight is associated with AD-type cognitive dysfunction, we hypothesized that moderate weight gain might have a protective effect on the bone loss in AD without exacerbating cognitive dysfunction.The bone mineral density, microarchitecture, and biomechanical properties of the femurs were then evaluated.These results suggest that a body weight gain induced by the HFD feeding regimen significantly improved bone mass in female APP/PS1 mice with no detriments to exploration ability and spatial memory, most likely via the action of elevated circulating leptin.

View Article: PubMed Central - PubMed

Affiliation: Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology and Frontier Institute of Science and Technology, Xi'an Jiaotong University , Xi'an , China.

ABSTRACT
Osteoporosis is negatively correlated with body mass, whereas both osteoporosis and weight loss occur at higher incidence during the progression of Alzheimer's disease (AD) than the age-matched non-dementia individuals. Given that there is no evidence that being overweight is associated with AD-type cognitive dysfunction, we hypothesized that moderate weight gain might have a protective effect on the bone loss in AD without exacerbating cognitive dysfunction. In this study, feeding a high-fat diet (HFD, 45% calorie from fat) to female APP/PS1 transgenic mice, an AD animal model, induced weight gain. The bone mineral density, microarchitecture, and biomechanical properties of the femurs were then evaluated. The results showed that the middle-aged female APP/PS1 transgenic mice were susceptible to osteoporosis of the femoral bones and that weight gain significantly enhanced bone mass and mechanical properties. Notably, HFD was not detrimental to brain insulin signaling and AβPP processing, as well as to exploration ability and working, learning, and memory performance of the transgenic mice measured by T maze and Morris water maze, compared with the mice fed a normal-fat diet (10% calorie from fat). In addition, the circulating levels of leptin but not estradiol were remarkably elevated in HFD-treated mice. These results suggest that a body weight gain induced by the HFD feeding regimen significantly improved bone mass in female APP/PS1 mice with no detriments to exploration ability and spatial memory, most likely via the action of elevated circulating leptin.

No MeSH data available.


Related in: MedlinePlus