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Innovative strategies for prediction and targeted prevention of glaucoma in healthy vasospastic individuals: context of neurodegenerative pathologies

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GON is a chronic degenerative process the onset of which is not possible to monitor by currently existing diagnostic tools... Early treatment has been reported to be highly beneficial for well-timed treatment measures to slow-down the disease progression... Comparative “Comet Assay” analysis revealed patterns of comets typical for glaucoma patients and some pattern similarities with vasospastic individuals, in contrast to controls as shown in Figure 1... Although DNA damage in the vasospastic non-glaucomatous group is not found to be significantly increased versus healthy controls, DNA from vasospastic individuals showed highly group-specific comet-patterns with the degree of damage intermediate between healthy controls and glaucoma patients... This predisposition should be thoroughly investigated and the specificity of “Comet Assay”-patterns of vasospastic individuals should be validated comparing with patterns of other degenerative and non-degenerative pathologies... Thus, “Comet Assay”-analysis as a suitable tool for biomarkers has also been suggested for another neurodegenerative disorder – Alzheimer’s disease. “Comet Assay”-analysis reveals enhanced DNA damage in both high- and normal-tension glaucoma... Whether the level of DNA-damage correlates with disease severity, or not remains currently unclear... Further studies should also evaluate, whether a significant increase in DNA damage of leukocytes of glaucoma patients is caused by either disease specific stress factors, such as local ischemic/reperfusion events, and/or decreased capacity of DNA-repair machinery.

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Ex vivo “Comet Assay“-analysis of DNA damage in circulating leukocytes of glaucoma patients and non-glaucomatous vasospastic individuals versus healthy controls [3]. Figures A-D give examples of images typical for A. control group, B. group of vasospastic individuals, C. group of normal-tension glaucoma patients, and D. group of high-tension glaucoma patients. Comet-patterns typical for healthy controls (A) show that chromosomal DNA is localised mainly to heads of comets (intact DNA). In contrast, images B, C, and D demonstrate clearly damaged DNA (visible comet-tails and diffuse comet-heads).
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Figure 1: Ex vivo “Comet Assay“-analysis of DNA damage in circulating leukocytes of glaucoma patients and non-glaucomatous vasospastic individuals versus healthy controls [3]. Figures A-D give examples of images typical for A. control group, B. group of vasospastic individuals, C. group of normal-tension glaucoma patients, and D. group of high-tension glaucoma patients. Comet-patterns typical for healthy controls (A) show that chromosomal DNA is localised mainly to heads of comets (intact DNA). In contrast, images B, C, and D demonstrate clearly damaged DNA (visible comet-tails and diffuse comet-heads).

Mentions: Comparative “Comet Assay” analysis revealed patterns of comets typical for glaucoma patients and some pattern similarities with vasospastic individuals, in contrast to controls as shown in Figure 1[3]. Although DNA damage in the vasospastic non-glaucomatous group is not found to be significantly increased versus healthy controls, DNA from vasospastic individuals showed highly group-specific comet-patterns with the degree of damage intermediate between healthy controls and glaucoma patients.


Innovative strategies for prediction and targeted prevention of glaucoma in healthy vasospastic individuals: context of neurodegenerative pathologies
Ex vivo “Comet Assay“-analysis of DNA damage in circulating leukocytes of glaucoma patients and non-glaucomatous vasospastic individuals versus healthy controls [3]. Figures A-D give examples of images typical for A. control group, B. group of vasospastic individuals, C. group of normal-tension glaucoma patients, and D. group of high-tension glaucoma patients. Comet-patterns typical for healthy controls (A) show that chromosomal DNA is localised mainly to heads of comets (intact DNA). In contrast, images B, C, and D demonstrate clearly damaged DNA (visible comet-tails and diffuse comet-heads).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4125906&req=5

Figure 1: Ex vivo “Comet Assay“-analysis of DNA damage in circulating leukocytes of glaucoma patients and non-glaucomatous vasospastic individuals versus healthy controls [3]. Figures A-D give examples of images typical for A. control group, B. group of vasospastic individuals, C. group of normal-tension glaucoma patients, and D. group of high-tension glaucoma patients. Comet-patterns typical for healthy controls (A) show that chromosomal DNA is localised mainly to heads of comets (intact DNA). In contrast, images B, C, and D demonstrate clearly damaged DNA (visible comet-tails and diffuse comet-heads).
Mentions: Comparative “Comet Assay” analysis revealed patterns of comets typical for glaucoma patients and some pattern similarities with vasospastic individuals, in contrast to controls as shown in Figure 1[3]. Although DNA damage in the vasospastic non-glaucomatous group is not found to be significantly increased versus healthy controls, DNA from vasospastic individuals showed highly group-specific comet-patterns with the degree of damage intermediate between healthy controls and glaucoma patients.

View Article: PubMed Central - HTML

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

GON is a chronic degenerative process the onset of which is not possible to monitor by currently existing diagnostic tools... Early treatment has been reported to be highly beneficial for well-timed treatment measures to slow-down the disease progression... Comparative “Comet Assay” analysis revealed patterns of comets typical for glaucoma patients and some pattern similarities with vasospastic individuals, in contrast to controls as shown in Figure 1... Although DNA damage in the vasospastic non-glaucomatous group is not found to be significantly increased versus healthy controls, DNA from vasospastic individuals showed highly group-specific comet-patterns with the degree of damage intermediate between healthy controls and glaucoma patients... This predisposition should be thoroughly investigated and the specificity of “Comet Assay”-patterns of vasospastic individuals should be validated comparing with patterns of other degenerative and non-degenerative pathologies... Thus, “Comet Assay”-analysis as a suitable tool for biomarkers has also been suggested for another neurodegenerative disorder – Alzheimer’s disease. “Comet Assay”-analysis reveals enhanced DNA damage in both high- and normal-tension glaucoma... Whether the level of DNA-damage correlates with disease severity, or not remains currently unclear... Further studies should also evaluate, whether a significant increase in DNA damage of leukocytes of glaucoma patients is caused by either disease specific stress factors, such as local ischemic/reperfusion events, and/or decreased capacity of DNA-repair machinery.

No MeSH data available.


Related in: MedlinePlus