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Cutinsomes and lipotubuloids appear to participate in cuticle formation in Ornithogalum umbellatum ovary epidermis: EM-immunogold research.

Kwiatkowska M, Wojtczak A, Popłońska K, Polit JT, Stępiński D, Domίnguez E, Heredia A - Protoplasma (2014)

Bottom Line: They were mostly found in the outer cell wall, the cuticular layer and the cuticle proper.A lower but still significant degree of labelling was also observed in lipotubuloids, cytoplasm and near plasmalemma of epidermal cells.Thus, we suggest that (1) cutinsomes could take part in the synthesis of cuticle components also in plant species other than tomato, (2) the lipotubuloids are the cytoplasmic domains connected with cuticle formation and (3) this process proceeds via cutinsomes.

View Article: PubMed Central - PubMed

Affiliation: Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Łódź, Pomorska 141/143, 90-236, Łódź, Poland, kwiat@biol.uni.lodz.pl.

ABSTRACT
The outer wall of Ornithogalum umbellatum ovary and the fruit epidermis are covered with a thick cuticle and contain lipotubuloids incorporating (3)H-palmitic acid. This was earlier evidenced by selective autoradiographic labelling of lipotubuloids. After post-incubation in a non-radioactive medium, some marked particles insoluble in organic solvents (similar to cutin matrix) moved to the cuticular layer. Hence, it was hypothesised that lipotubuloids participated in cuticle synthesis. It was previously suggested that cutinsomes, nanoparticles containing polyhydroxy fatty acids, formed the cuticle. Thus, identification of the cutinsomes in O. umbellatum ovary epidermal cells, including lipotubuloids, was undertaken in order to verify the idea of lipotubuloid participation in cuticle synthesis in this species. Electron microscopy and immunogold method with the antibodies recognizing cutinsomes were used to identify these structures. They were mostly found in the outer cell wall, the cuticular layer and the cuticle proper. A lower but still significant degree of labelling was also observed in lipotubuloids, cytoplasm and near plasmalemma of epidermal cells. It seems that cutinsomes are formed in lipotubuloids and then they leave them and move towards the cuticle in epidermal cells of O. umbellatum ovary. Thus, we suggest that (1) cutinsomes could take part in the synthesis of cuticle components also in plant species other than tomato, (2) the lipotubuloids are the cytoplasmic domains connected with cuticle formation and (3) this process proceeds via cutinsomes.

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a–c Cutinsome-like structures in the cell wall of O. umbellatum ovary epidermal cells identified by conventional electron microscopy (sections not treated with H2O2). Scale bar, 50 nm
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Fig2: a–c Cutinsome-like structures in the cell wall of O. umbellatum ovary epidermal cells identified by conventional electron microscopy (sections not treated with H2O2). Scale bar, 50 nm

Mentions: EM morphological observations at a large magnification (×50,000 original magnification) of lipotubuloid revealed dark structures resembling cutinsomes, i.e. in vitro-derived polyhydroxy fatty acid nanoparticles, which were demonstrated by Heredia-Guerrero et al. (2011) using electron microscopy techniques. These cutinsomes were osmiophilic structures of 50–200 nm in size. They assumed various shapes, sometimes irregular, with more or less pronounced dark contour (see Fig. 2 in Heredia-Guerrero et al. 2011). In O. umbellatum aerial epidermal cells, cutinsome-like structures were of similar sizes and were located in lipotubuloids, external cell wall (Fig. 2a–c), cytoplasm, near lipid bodies, ER, microtubules and plasmalemma. They were also observed in the cuticular layer and in the area of cuticle proper. Due to the lack of precise morphological criteria for cutinsomes, cutinsome-like structures might be regarded as artefacts. For this reason, it was necessary to undertake their identification using the immunogold method with the use of antibodies against cutinsomes.Fig. 2


Cutinsomes and lipotubuloids appear to participate in cuticle formation in Ornithogalum umbellatum ovary epidermis: EM-immunogold research.

Kwiatkowska M, Wojtczak A, Popłońska K, Polit JT, Stępiński D, Domίnguez E, Heredia A - Protoplasma (2014)

a–c Cutinsome-like structures in the cell wall of O. umbellatum ovary epidermal cells identified by conventional electron microscopy (sections not treated with H2O2). Scale bar, 50 nm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4125816&req=5

Fig2: a–c Cutinsome-like structures in the cell wall of O. umbellatum ovary epidermal cells identified by conventional electron microscopy (sections not treated with H2O2). Scale bar, 50 nm
Mentions: EM morphological observations at a large magnification (×50,000 original magnification) of lipotubuloid revealed dark structures resembling cutinsomes, i.e. in vitro-derived polyhydroxy fatty acid nanoparticles, which were demonstrated by Heredia-Guerrero et al. (2011) using electron microscopy techniques. These cutinsomes were osmiophilic structures of 50–200 nm in size. They assumed various shapes, sometimes irregular, with more or less pronounced dark contour (see Fig. 2 in Heredia-Guerrero et al. 2011). In O. umbellatum aerial epidermal cells, cutinsome-like structures were of similar sizes and were located in lipotubuloids, external cell wall (Fig. 2a–c), cytoplasm, near lipid bodies, ER, microtubules and plasmalemma. They were also observed in the cuticular layer and in the area of cuticle proper. Due to the lack of precise morphological criteria for cutinsomes, cutinsome-like structures might be regarded as artefacts. For this reason, it was necessary to undertake their identification using the immunogold method with the use of antibodies against cutinsomes.Fig. 2

Bottom Line: They were mostly found in the outer cell wall, the cuticular layer and the cuticle proper.A lower but still significant degree of labelling was also observed in lipotubuloids, cytoplasm and near plasmalemma of epidermal cells.Thus, we suggest that (1) cutinsomes could take part in the synthesis of cuticle components also in plant species other than tomato, (2) the lipotubuloids are the cytoplasmic domains connected with cuticle formation and (3) this process proceeds via cutinsomes.

View Article: PubMed Central - PubMed

Affiliation: Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Łódź, Pomorska 141/143, 90-236, Łódź, Poland, kwiat@biol.uni.lodz.pl.

ABSTRACT
The outer wall of Ornithogalum umbellatum ovary and the fruit epidermis are covered with a thick cuticle and contain lipotubuloids incorporating (3)H-palmitic acid. This was earlier evidenced by selective autoradiographic labelling of lipotubuloids. After post-incubation in a non-radioactive medium, some marked particles insoluble in organic solvents (similar to cutin matrix) moved to the cuticular layer. Hence, it was hypothesised that lipotubuloids participated in cuticle synthesis. It was previously suggested that cutinsomes, nanoparticles containing polyhydroxy fatty acids, formed the cuticle. Thus, identification of the cutinsomes in O. umbellatum ovary epidermal cells, including lipotubuloids, was undertaken in order to verify the idea of lipotubuloid participation in cuticle synthesis in this species. Electron microscopy and immunogold method with the antibodies recognizing cutinsomes were used to identify these structures. They were mostly found in the outer cell wall, the cuticular layer and the cuticle proper. A lower but still significant degree of labelling was also observed in lipotubuloids, cytoplasm and near plasmalemma of epidermal cells. It seems that cutinsomes are formed in lipotubuloids and then they leave them and move towards the cuticle in epidermal cells of O. umbellatum ovary. Thus, we suggest that (1) cutinsomes could take part in the synthesis of cuticle components also in plant species other than tomato, (2) the lipotubuloids are the cytoplasmic domains connected with cuticle formation and (3) this process proceeds via cutinsomes.

Show MeSH