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Cutinsomes and lipotubuloids appear to participate in cuticle formation in Ornithogalum umbellatum ovary epidermis: EM-immunogold research.

Kwiatkowska M, Wojtczak A, Popłońska K, Polit JT, Stępiński D, Domίnguez E, Heredia A - Protoplasma (2014)

Bottom Line: They were mostly found in the outer cell wall, the cuticular layer and the cuticle proper.A lower but still significant degree of labelling was also observed in lipotubuloids, cytoplasm and near plasmalemma of epidermal cells.Thus, we suggest that (1) cutinsomes could take part in the synthesis of cuticle components also in plant species other than tomato, (2) the lipotubuloids are the cytoplasmic domains connected with cuticle formation and (3) this process proceeds via cutinsomes.

View Article: PubMed Central - PubMed

Affiliation: Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Łódź, Pomorska 141/143, 90-236, Łódź, Poland, kwiat@biol.uni.lodz.pl.

ABSTRACT
The outer wall of Ornithogalum umbellatum ovary and the fruit epidermis are covered with a thick cuticle and contain lipotubuloids incorporating (3)H-palmitic acid. This was earlier evidenced by selective autoradiographic labelling of lipotubuloids. After post-incubation in a non-radioactive medium, some marked particles insoluble in organic solvents (similar to cutin matrix) moved to the cuticular layer. Hence, it was hypothesised that lipotubuloids participated in cuticle synthesis. It was previously suggested that cutinsomes, nanoparticles containing polyhydroxy fatty acids, formed the cuticle. Thus, identification of the cutinsomes in O. umbellatum ovary epidermal cells, including lipotubuloids, was undertaken in order to verify the idea of lipotubuloid participation in cuticle synthesis in this species. Electron microscopy and immunogold method with the antibodies recognizing cutinsomes were used to identify these structures. They were mostly found in the outer cell wall, the cuticular layer and the cuticle proper. A lower but still significant degree of labelling was also observed in lipotubuloids, cytoplasm and near plasmalemma of epidermal cells. It seems that cutinsomes are formed in lipotubuloids and then they leave them and move towards the cuticle in epidermal cells of O. umbellatum ovary. Thus, we suggest that (1) cutinsomes could take part in the synthesis of cuticle components also in plant species other than tomato, (2) the lipotubuloids are the cytoplasmic domains connected with cuticle formation and (3) this process proceeds via cutinsomes.

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Scheme of O. umbellatum ovary epidermal cell from blooming flowers. a Longitudinal section of the cell; vertical line indicates the site of cross-section. b Cross-section of the cell at the site of lipotubuloid placement. c cytoplasm, cu cuticle, l lipotubuloid, lb lipid bodies, n nucleus, t tonoplast, v vacuole, w polysaccharide wall
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Fig1: Scheme of O. umbellatum ovary epidermal cell from blooming flowers. a Longitudinal section of the cell; vertical line indicates the site of cross-section. b Cross-section of the cell at the site of lipotubuloid placement. c cytoplasm, cu cuticle, l lipotubuloid, lb lipid bodies, n nucleus, t tonoplast, v vacuole, w polysaccharide wall

Mentions: Our previous studies were mainly carried out on longitudinal epidermis sections allowing the in vivo observation of lipotubuloids. In the present studies, cross-sections were necessary to visualize the relation between lipotubuloids and cuticle. The experiments were conducted on intensively growing ovary epidermis of blooming flowers. Figure 1 shows the mutual spatial relation between lipotubuloids, outer epidermal wall and cuticle in longitudinal (Fig. 1a) and cross (Fig. 1b) sections. A lipotubuloid dynamically moving and rotating around its changing axis in a cell was caught during fixation and is visible on the ultrathin cross-section near the outer epidermal wall to which it was connected via the cytoplasm.Fig. 1


Cutinsomes and lipotubuloids appear to participate in cuticle formation in Ornithogalum umbellatum ovary epidermis: EM-immunogold research.

Kwiatkowska M, Wojtczak A, Popłońska K, Polit JT, Stępiński D, Domίnguez E, Heredia A - Protoplasma (2014)

Scheme of O. umbellatum ovary epidermal cell from blooming flowers. a Longitudinal section of the cell; vertical line indicates the site of cross-section. b Cross-section of the cell at the site of lipotubuloid placement. c cytoplasm, cu cuticle, l lipotubuloid, lb lipid bodies, n nucleus, t tonoplast, v vacuole, w polysaccharide wall
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4125816&req=5

Fig1: Scheme of O. umbellatum ovary epidermal cell from blooming flowers. a Longitudinal section of the cell; vertical line indicates the site of cross-section. b Cross-section of the cell at the site of lipotubuloid placement. c cytoplasm, cu cuticle, l lipotubuloid, lb lipid bodies, n nucleus, t tonoplast, v vacuole, w polysaccharide wall
Mentions: Our previous studies were mainly carried out on longitudinal epidermis sections allowing the in vivo observation of lipotubuloids. In the present studies, cross-sections were necessary to visualize the relation between lipotubuloids and cuticle. The experiments were conducted on intensively growing ovary epidermis of blooming flowers. Figure 1 shows the mutual spatial relation between lipotubuloids, outer epidermal wall and cuticle in longitudinal (Fig. 1a) and cross (Fig. 1b) sections. A lipotubuloid dynamically moving and rotating around its changing axis in a cell was caught during fixation and is visible on the ultrathin cross-section near the outer epidermal wall to which it was connected via the cytoplasm.Fig. 1

Bottom Line: They were mostly found in the outer cell wall, the cuticular layer and the cuticle proper.A lower but still significant degree of labelling was also observed in lipotubuloids, cytoplasm and near plasmalemma of epidermal cells.Thus, we suggest that (1) cutinsomes could take part in the synthesis of cuticle components also in plant species other than tomato, (2) the lipotubuloids are the cytoplasmic domains connected with cuticle formation and (3) this process proceeds via cutinsomes.

View Article: PubMed Central - PubMed

Affiliation: Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Łódź, Pomorska 141/143, 90-236, Łódź, Poland, kwiat@biol.uni.lodz.pl.

ABSTRACT
The outer wall of Ornithogalum umbellatum ovary and the fruit epidermis are covered with a thick cuticle and contain lipotubuloids incorporating (3)H-palmitic acid. This was earlier evidenced by selective autoradiographic labelling of lipotubuloids. After post-incubation in a non-radioactive medium, some marked particles insoluble in organic solvents (similar to cutin matrix) moved to the cuticular layer. Hence, it was hypothesised that lipotubuloids participated in cuticle synthesis. It was previously suggested that cutinsomes, nanoparticles containing polyhydroxy fatty acids, formed the cuticle. Thus, identification of the cutinsomes in O. umbellatum ovary epidermal cells, including lipotubuloids, was undertaken in order to verify the idea of lipotubuloid participation in cuticle synthesis in this species. Electron microscopy and immunogold method with the antibodies recognizing cutinsomes were used to identify these structures. They were mostly found in the outer cell wall, the cuticular layer and the cuticle proper. A lower but still significant degree of labelling was also observed in lipotubuloids, cytoplasm and near plasmalemma of epidermal cells. It seems that cutinsomes are formed in lipotubuloids and then they leave them and move towards the cuticle in epidermal cells of O. umbellatum ovary. Thus, we suggest that (1) cutinsomes could take part in the synthesis of cuticle components also in plant species other than tomato, (2) the lipotubuloids are the cytoplasmic domains connected with cuticle formation and (3) this process proceeds via cutinsomes.

Show MeSH