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Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil.

Pacwa-Płociniczak M, Płaza GA, Poliwoda A, Piotrowska-Seget Z - Environ Sci Pollut Res Int (2014)

Bottom Line: The Pseudomonas sp.Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported.Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Silesia, Jagiellońska 28, 40-032, Katowice, Poland, mpacwa@us.edu.pl.

ABSTRACT
The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27, 39, 27 and 13% of hydrocarbons added to culture medium were degraded, respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils.

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Related in: MedlinePlus

NMR spectra of crude biosurfactant produced by P-1 strain cultivated in molasses medium: a1H NMR; b13C NMR
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Fig6: NMR spectra of crude biosurfactant produced by P-1 strain cultivated in molasses medium: a1H NMR; b13C NMR

Mentions: The chemical structure of the rhamnolipids present in the analysed mixture of biosurfactants was confirmed by nuclear magnetic resonance spectroscopy. The obtained 1H NMR spectra strongly indicated that the brownish oily substance produced by P-1 was a mixture of glycolipids, and not another group of biosurfactants (Fig. 6a). In the 13C-NMR spectrum lipid, signals of CH2 from δ 21.9 to 31.1 and CH3 at δ 13.8, and ester and carboxylic signals at δ 171.5 and δ 176.95 were observed (Fig. 6b).Fig. 6


Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil.

Pacwa-Płociniczak M, Płaza GA, Poliwoda A, Piotrowska-Seget Z - Environ Sci Pollut Res Int (2014)

NMR spectra of crude biosurfactant produced by P-1 strain cultivated in molasses medium: a1H NMR; b13C NMR
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4125813&req=5

Fig6: NMR spectra of crude biosurfactant produced by P-1 strain cultivated in molasses medium: a1H NMR; b13C NMR
Mentions: The chemical structure of the rhamnolipids present in the analysed mixture of biosurfactants was confirmed by nuclear magnetic resonance spectroscopy. The obtained 1H NMR spectra strongly indicated that the brownish oily substance produced by P-1 was a mixture of glycolipids, and not another group of biosurfactants (Fig. 6a). In the 13C-NMR spectrum lipid, signals of CH2 from δ 21.9 to 31.1 and CH3 at δ 13.8, and ester and carboxylic signals at δ 171.5 and δ 176.95 were observed (Fig. 6b).Fig. 6

Bottom Line: The Pseudomonas sp.Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported.Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Silesia, Jagiellońska 28, 40-032, Katowice, Poland, mpacwa@us.edu.pl.

ABSTRACT
The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27, 39, 27 and 13% of hydrocarbons added to culture medium were degraded, respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils.

Show MeSH
Related in: MedlinePlus