Limits...
Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil.

Pacwa-Płociniczak M, Płaza GA, Poliwoda A, Piotrowska-Seget Z - Environ Sci Pollut Res Int (2014)

Bottom Line: The Pseudomonas sp.Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported.Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Silesia, Jagiellońska 28, 40-032, Katowice, Poland, mpacwa@us.edu.pl.

ABSTRACT
The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27, 39, 27 and 13% of hydrocarbons added to culture medium were degraded, respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils.

Show MeSH

Related in: MedlinePlus

FT-IR spectrum of the crude biosurfactant produced by P-1 strain cultivated in molasses medium
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4125813&req=5

Fig5: FT-IR spectrum of the crude biosurfactant produced by P-1 strain cultivated in molasses medium

Mentions: The molecular composition of the biosurfactant produced by Pseudomonas sp. P-1 was evaluated by FT-IR. The obtained infrared spectrum disclosed a broad stretching peak at 3,370 cm−1, indicating the presence of –OH bonds (free hydroxyl groups of rhamnose rings). Absorption around 2,929 and 2,858 cm−1 is assigned to the symmetric stretch (–CH) of –CH2 and –CH3 groups of aliphatic chains. The absorption peak located at 1,734 cm−1 indicates the presence of ester carbonyl groups (–C = O bond) in biosurfactants. The ester carbonyl group from the bands at 1,128 cm−1 and C–O–C vibrations at 1,053 cm−1 (rhamnose rings) were also proved. Protein-related bands, the –C = O amide I (1,659 cm−1) and –NH/–C = O combination of the amide II bands (1,537 cm−1), were observed. It might be possible that the additional bands at 1,659 and 1,537 cm−1 resulted from polypeptide contamination resulting from cell debris co-precipitated with the biosurfactant during the extraction process. The absorption peak at 994 cm−1 may indicate the presence of polysaccharide or polysaccharide-like substances in the biosurfactants (Fig. 5)Fig 5


Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil.

Pacwa-Płociniczak M, Płaza GA, Poliwoda A, Piotrowska-Seget Z - Environ Sci Pollut Res Int (2014)

FT-IR spectrum of the crude biosurfactant produced by P-1 strain cultivated in molasses medium
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4125813&req=5

Fig5: FT-IR spectrum of the crude biosurfactant produced by P-1 strain cultivated in molasses medium
Mentions: The molecular composition of the biosurfactant produced by Pseudomonas sp. P-1 was evaluated by FT-IR. The obtained infrared spectrum disclosed a broad stretching peak at 3,370 cm−1, indicating the presence of –OH bonds (free hydroxyl groups of rhamnose rings). Absorption around 2,929 and 2,858 cm−1 is assigned to the symmetric stretch (–CH) of –CH2 and –CH3 groups of aliphatic chains. The absorption peak located at 1,734 cm−1 indicates the presence of ester carbonyl groups (–C = O bond) in biosurfactants. The ester carbonyl group from the bands at 1,128 cm−1 and C–O–C vibrations at 1,053 cm−1 (rhamnose rings) were also proved. Protein-related bands, the –C = O amide I (1,659 cm−1) and –NH/–C = O combination of the amide II bands (1,537 cm−1), were observed. It might be possible that the additional bands at 1,659 and 1,537 cm−1 resulted from polypeptide contamination resulting from cell debris co-precipitated with the biosurfactant during the extraction process. The absorption peak at 994 cm−1 may indicate the presence of polysaccharide or polysaccharide-like substances in the biosurfactants (Fig. 5)Fig 5

Bottom Line: The Pseudomonas sp.Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported.Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Silesia, Jagiellońska 28, 40-032, Katowice, Poland, mpacwa@us.edu.pl.

ABSTRACT
The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27, 39, 27 and 13% of hydrocarbons added to culture medium were degraded, respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils.

Show MeSH
Related in: MedlinePlus