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Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil.

Pacwa-Płociniczak M, Płaza GA, Poliwoda A, Piotrowska-Seget Z - Environ Sci Pollut Res Int (2014)

Bottom Line: The Pseudomonas sp.Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported.Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Silesia, Jagiellońska 28, 40-032, Katowice, Poland, mpacwa@us.edu.pl.

ABSTRACT
The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27, 39, 27 and 13% of hydrocarbons added to culture medium were degraded, respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils.

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Related in: MedlinePlus

Degradation of hydrocarbons by P-1 strain
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Fig4: Degradation of hydrocarbons by P-1 strain

Mentions: The use of the polymerase chain reaction and the sets of primers alkB and nahAc-7 confirmed the presence of genes involved in the degradation of alkanes and naphthalene in the genome of the P-1 strain. In the gel (Fig. 3a, b), both DNA products of the expected size, 100 and 136 bp, respectively, were observed. The ability of P-1 to degrade crude oil, fractions A5 and P3 of crude oil, hexadecane and phenanthrene was determined by measuring the amount of residual hydrocarbons in the culture medium after 28 days of culture. Among the analyzed hydrocarbons crude oil, fractions A5 and P3 of crude oil and hexadecane were degraded with various efficiency, and no degradation of hydrocarbon was observed in the medium supplemented with phenanthrene (96 % of phenanthrene remained in the culture) as a sole carbon source. The highest degradation efficiency was observed in the culture supplemented with fraction A5 of crude oil (39 % of hydrocarbon was degraded at the end of the experiment). In the media supplemented with crude oil and fraction P3 of crude oil, 27 % of hydrocarbons were degraded and only 13 % of hexadecane was degraded in the medium with this hydrocarbon as the only carbon source (Fig. 4).Fig. 3


Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil.

Pacwa-Płociniczak M, Płaza GA, Poliwoda A, Piotrowska-Seget Z - Environ Sci Pollut Res Int (2014)

Degradation of hydrocarbons by P-1 strain
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4125813&req=5

Fig4: Degradation of hydrocarbons by P-1 strain
Mentions: The use of the polymerase chain reaction and the sets of primers alkB and nahAc-7 confirmed the presence of genes involved in the degradation of alkanes and naphthalene in the genome of the P-1 strain. In the gel (Fig. 3a, b), both DNA products of the expected size, 100 and 136 bp, respectively, were observed. The ability of P-1 to degrade crude oil, fractions A5 and P3 of crude oil, hexadecane and phenanthrene was determined by measuring the amount of residual hydrocarbons in the culture medium after 28 days of culture. Among the analyzed hydrocarbons crude oil, fractions A5 and P3 of crude oil and hexadecane were degraded with various efficiency, and no degradation of hydrocarbon was observed in the medium supplemented with phenanthrene (96 % of phenanthrene remained in the culture) as a sole carbon source. The highest degradation efficiency was observed in the culture supplemented with fraction A5 of crude oil (39 % of hydrocarbon was degraded at the end of the experiment). In the media supplemented with crude oil and fraction P3 of crude oil, 27 % of hydrocarbons were degraded and only 13 % of hexadecane was degraded in the medium with this hydrocarbon as the only carbon source (Fig. 4).Fig. 3

Bottom Line: The Pseudomonas sp.Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported.Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Silesia, Jagiellońska 28, 40-032, Katowice, Poland, mpacwa@us.edu.pl.

ABSTRACT
The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27, 39, 27 and 13% of hydrocarbons added to culture medium were degraded, respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils.

Show MeSH
Related in: MedlinePlus