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Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil.

Pacwa-Płociniczak M, Płaza GA, Poliwoda A, Piotrowska-Seget Z - Environ Sci Pollut Res Int (2014)

Bottom Line: The Pseudomonas sp.Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported.Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Silesia, Jagiellońska 28, 40-032, Katowice, Poland, mpacwa@us.edu.pl.

ABSTRACT
The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27, 39, 27 and 13% of hydrocarbons added to culture medium were degraded, respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils.

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Related in: MedlinePlus

Neighbour-joining phylogenetic tree of bacteria based on 16S rRNA gene sequence comparisons. Bootstrap values are indicated at the branches from 1,000 replications. GenBank accession numbers are given in parentheses
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Related In: Results  -  Collection


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Fig2: Neighbour-joining phylogenetic tree of bacteria based on 16S rRNA gene sequence comparisons. Bootstrap values are indicated at the branches from 1,000 replications. GenBank accession numbers are given in parentheses

Mentions: The strain was identified using 16S rDNA gene sequence analysis as Pseudomonas sp. and designed as the P-1 strain. The phylogenetic analysis showed that the 16S rDNA sequence of P-1 had 99 % sequence similarity with strains Pseudomonas aeruginosa DSM 50071 and P. aeruginosa PAO1 (Fig. 2).Fig. 2


Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil.

Pacwa-Płociniczak M, Płaza GA, Poliwoda A, Piotrowska-Seget Z - Environ Sci Pollut Res Int (2014)

Neighbour-joining phylogenetic tree of bacteria based on 16S rRNA gene sequence comparisons. Bootstrap values are indicated at the branches from 1,000 replications. GenBank accession numbers are given in parentheses
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4125813&req=5

Fig2: Neighbour-joining phylogenetic tree of bacteria based on 16S rRNA gene sequence comparisons. Bootstrap values are indicated at the branches from 1,000 replications. GenBank accession numbers are given in parentheses
Mentions: The strain was identified using 16S rDNA gene sequence analysis as Pseudomonas sp. and designed as the P-1 strain. The phylogenetic analysis showed that the 16S rDNA sequence of P-1 had 99 % sequence similarity with strains Pseudomonas aeruginosa DSM 50071 and P. aeruginosa PAO1 (Fig. 2).Fig. 2

Bottom Line: The Pseudomonas sp.Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported.Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Silesia, Jagiellońska 28, 40-032, Katowice, Poland, mpacwa@us.edu.pl.

ABSTRACT
The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27, 39, 27 and 13% of hydrocarbons added to culture medium were degraded, respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils.

Show MeSH
Related in: MedlinePlus