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Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil.

Pacwa-Płociniczak M, Płaza GA, Poliwoda A, Piotrowska-Seget Z - Environ Sci Pollut Res Int (2014)

Bottom Line: The Pseudomonas sp.Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported.Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Silesia, Jagiellońska 28, 40-032, Katowice, Poland, mpacwa@us.edu.pl.

ABSTRACT
The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27, 39, 27 and 13% of hydrocarbons added to culture medium were degraded, respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils.

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Related in: MedlinePlus

Agarose gel electrophoresis of PCR products of rhamnosyl transferase gene; M—1 kb DNA ladder; P-1—Pseudomonas sp. P-1 strain; Control—sample without DNA (1 % agarose gel)
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Related In: Results  -  Collection


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Fig1: Agarose gel electrophoresis of PCR products of rhamnosyl transferase gene; M—1 kb DNA ladder; P-1—Pseudomonas sp. P-1 strain; Control—sample without DNA (1 % agarose gel)

Mentions: The application of an enrichment technique for the isolation of bacteria from petroleum-polluted soil from the area of 100-year-old oil refinery in Czechowice-Dziedzice (Poland) enabled us to find several bacterial strains with the ability to degrade hydrocarbons and produce biosurfactants. However, only one strain (P-1) demonstrated a positive reaction for both blood agar and methylene blue agar tests, suggesting the potential ability of the isolate to synthesize glycolipid biosurfactant. This ability was confirmed by the polymerase chain reaction of the rhl gene. When the primers rhlABF and rhlABR were used, a single DNA fragment of the expected size (777 bp) was amplified in the P-1 strain, and no amplification was observed in the control without DNA (Fig. 1).Fig. 1


Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil.

Pacwa-Płociniczak M, Płaza GA, Poliwoda A, Piotrowska-Seget Z - Environ Sci Pollut Res Int (2014)

Agarose gel electrophoresis of PCR products of rhamnosyl transferase gene; M—1 kb DNA ladder; P-1—Pseudomonas sp. P-1 strain; Control—sample without DNA (1 % agarose gel)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4125813&req=5

Fig1: Agarose gel electrophoresis of PCR products of rhamnosyl transferase gene; M—1 kb DNA ladder; P-1—Pseudomonas sp. P-1 strain; Control—sample without DNA (1 % agarose gel)
Mentions: The application of an enrichment technique for the isolation of bacteria from petroleum-polluted soil from the area of 100-year-old oil refinery in Czechowice-Dziedzice (Poland) enabled us to find several bacterial strains with the ability to degrade hydrocarbons and produce biosurfactants. However, only one strain (P-1) demonstrated a positive reaction for both blood agar and methylene blue agar tests, suggesting the potential ability of the isolate to synthesize glycolipid biosurfactant. This ability was confirmed by the polymerase chain reaction of the rhl gene. When the primers rhlABF and rhlABR were used, a single DNA fragment of the expected size (777 bp) was amplified in the P-1 strain, and no amplification was observed in the control without DNA (Fig. 1).Fig. 1

Bottom Line: The Pseudomonas sp.Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported.Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Silesia, Jagiellońska 28, 40-032, Katowice, Poland, mpacwa@us.edu.pl.

ABSTRACT
The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27, 39, 27 and 13% of hydrocarbons added to culture medium were degraded, respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils.

Show MeSH
Related in: MedlinePlus