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Changes in the cholinergic innervation pattern of porcine ovaries with cysts induced by dexamethasone administration.

Kozłowska A, Majewski M, Jana B - J. Mol. Neurosci. (2014)

Bottom Line: We revealed earlier that induction of ovarian cysts in gilts by dexamethasone phosphate disodium salt (DXM) administration from the follicular phase of the estrous cycle (EC) changed the cholinergic innervation of the gonad.In the cystic ovaries, VAChT-, nNOS- and SOM-IR fibres were found around cysts and small tertiary follicles; nNOS-IR and also VAChT-IR fibres were observed near secondary follicles and veins; and VAChT- and nNOS-IR fibres were not found around cortical arteries.The number of VIP-IR fibres increased near the cysts and within the ground plexus, while the number of VAChT-IR fibres decreased within the medullar part of this structure.

View Article: PubMed Central - PubMed

Affiliation: Division of Reproductive Biology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland, kozlowska.anna@uwm.edu.pl.

ABSTRACT
We revealed earlier that induction of ovarian cysts in gilts by dexamethasone phosphate disodium salt (DXM) administration from the follicular phase of the estrous cycle (EC) changed the cholinergic innervation of the gonad. In the present study, the innervation of porcine ovaries by vesicular acetylcholine transporter (VAChT)-, neuronal nitric oxide synthase (nNOS)-, vasoactive intestinal peptide (VIP)- and somatostatin (SOM)-immunoreactive (IR) fibres, after induction of cystic changes from the middle luteal phase of the EC, was determined. The cystic changes were induced by DXM injections from days 7 to 21 of the EC, and 11 days later, the ovaries were collected. In the cystic ovaries, VAChT-, nNOS- and SOM-IR fibres were found around cysts and small tertiary follicles; nNOS-IR and also VAChT-IR fibres were observed near secondary follicles and veins; and VAChT- and nNOS-IR fibres were not found around cortical arteries. The number of VIP-IR fibres increased near the cysts and within the ground plexus, while the number of VAChT-IR fibres decreased within the medullar part of this structure. Thus, our study showed changes in the cholinergic innervation pattern of the porcine cystic ovaries induced from the middle phase of the cycle and confirmed that cystic ovary innervation depends partly on the phase of the EC in which the induction of cysts was started.

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Immunohistochemical localization of SOM-IR nerve fibres in the medullar part of the control (CON) and DXM-treated (DXM) gilts. An increase in the number of the SOM-IR nerve fibres in the area of the ground plexus in the DXM group (31) compared to the CON group (30). Arrows, nerve fibres. Scale bar 25 μm
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Fig6: Immunohistochemical localization of SOM-IR nerve fibres in the medullar part of the control (CON) and DXM-treated (DXM) gilts. An increase in the number of the SOM-IR nerve fibres in the area of the ground plexus in the DXM group (31) compared to the CON group (30). Arrows, nerve fibres. Scale bar 25 μm

Mentions: In the DXM group, the numbers of nerve terminals expressing VIP (Fig. 24) and SOM (Fig. 31) in the area of the ground plexus were higher (P < 0.001), whereas the number of VAChT-IR fibres (Fig. 9a) was lower (P < 0.01) than those found in the CON group (Figs. 23—VIP, 30—SOM and 8a—VAChT, respectively). Moreover, following DXM administration, nNOS-IR terminals were not present around arteries (Fig. 11), while these were present in the CON group (Fig. 10). The populations of nNOS-IR nerve fibres within the ground plexus in the CON and DXM groups were similar. Furthermore, these fibres formed fascicles in the cystic ovaries (Fig. 9b), in contrast to the control gonad (Fig. 8b). We did not observe any differences in the number of VIP-IR around the blood vessel, and VAChT- and SOM-IR fibres were not found. Moreover, veins were not supplied by nNOS-IR nerve fibres in all the gilts studied.Figs. 8–11


Changes in the cholinergic innervation pattern of porcine ovaries with cysts induced by dexamethasone administration.

Kozłowska A, Majewski M, Jana B - J. Mol. Neurosci. (2014)

Immunohistochemical localization of SOM-IR nerve fibres in the medullar part of the control (CON) and DXM-treated (DXM) gilts. An increase in the number of the SOM-IR nerve fibres in the area of the ground plexus in the DXM group (31) compared to the CON group (30). Arrows, nerve fibres. Scale bar 25 μm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4125811&req=5

Fig6: Immunohistochemical localization of SOM-IR nerve fibres in the medullar part of the control (CON) and DXM-treated (DXM) gilts. An increase in the number of the SOM-IR nerve fibres in the area of the ground plexus in the DXM group (31) compared to the CON group (30). Arrows, nerve fibres. Scale bar 25 μm
Mentions: In the DXM group, the numbers of nerve terminals expressing VIP (Fig. 24) and SOM (Fig. 31) in the area of the ground plexus were higher (P < 0.001), whereas the number of VAChT-IR fibres (Fig. 9a) was lower (P < 0.01) than those found in the CON group (Figs. 23—VIP, 30—SOM and 8a—VAChT, respectively). Moreover, following DXM administration, nNOS-IR terminals were not present around arteries (Fig. 11), while these were present in the CON group (Fig. 10). The populations of nNOS-IR nerve fibres within the ground plexus in the CON and DXM groups were similar. Furthermore, these fibres formed fascicles in the cystic ovaries (Fig. 9b), in contrast to the control gonad (Fig. 8b). We did not observe any differences in the number of VIP-IR around the blood vessel, and VAChT- and SOM-IR fibres were not found. Moreover, veins were not supplied by nNOS-IR nerve fibres in all the gilts studied.Figs. 8–11

Bottom Line: We revealed earlier that induction of ovarian cysts in gilts by dexamethasone phosphate disodium salt (DXM) administration from the follicular phase of the estrous cycle (EC) changed the cholinergic innervation of the gonad.In the cystic ovaries, VAChT-, nNOS- and SOM-IR fibres were found around cysts and small tertiary follicles; nNOS-IR and also VAChT-IR fibres were observed near secondary follicles and veins; and VAChT- and nNOS-IR fibres were not found around cortical arteries.The number of VIP-IR fibres increased near the cysts and within the ground plexus, while the number of VAChT-IR fibres decreased within the medullar part of this structure.

View Article: PubMed Central - PubMed

Affiliation: Division of Reproductive Biology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland, kozlowska.anna@uwm.edu.pl.

ABSTRACT
We revealed earlier that induction of ovarian cysts in gilts by dexamethasone phosphate disodium salt (DXM) administration from the follicular phase of the estrous cycle (EC) changed the cholinergic innervation of the gonad. In the present study, the innervation of porcine ovaries by vesicular acetylcholine transporter (VAChT)-, neuronal nitric oxide synthase (nNOS)-, vasoactive intestinal peptide (VIP)- and somatostatin (SOM)-immunoreactive (IR) fibres, after induction of cystic changes from the middle luteal phase of the EC, was determined. The cystic changes were induced by DXM injections from days 7 to 21 of the EC, and 11 days later, the ovaries were collected. In the cystic ovaries, VAChT-, nNOS- and SOM-IR fibres were found around cysts and small tertiary follicles; nNOS-IR and also VAChT-IR fibres were observed near secondary follicles and veins; and VAChT- and nNOS-IR fibres were not found around cortical arteries. The number of VIP-IR fibres increased near the cysts and within the ground plexus, while the number of VAChT-IR fibres decreased within the medullar part of this structure. Thus, our study showed changes in the cholinergic innervation pattern of the porcine cystic ovaries induced from the middle phase of the cycle and confirmed that cystic ovary innervation depends partly on the phase of the EC in which the induction of cysts was started.

Show MeSH
Related in: MedlinePlus