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Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy.

Matei C, Tampa M, Caruntu C, Ion RM, Georgescu SR, Dumitrascu GR, Constantin C, Neagu M - Biol. Res. (2014)

Bottom Line: The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential.The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis.Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Photodynamic therapy is an alternative treatment of muco-cutaneous tumors that uses a light source able to photoactivate a chemical compound that acts as a photosensitizer. The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential.

Results: The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis. Using protein microarray we evaluate the possible molecular pathways by which photodynamic therapy activates apoptosis in dysplastic oral keratinocytes cells, leading to the tumoral cells destruction. Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude.

Conclusions: Up to date, the intimate molecular apoptotic mechanisms activated by photodynamic therapy with this type of phthalocyanine in dysplastic human oral keratinocytes are not completely elucidated. With protein microarray as high-throughput proteomic approach a better understanding of the manner in which photodynamic therapy leads to tumoral cell destruction can be obtained, by depicting apoptotic molecules that can be potentially triggered in future anti-tumoral therapies.

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Results of protein microarray analysis of the main proteins involved in apoptosis in DOK cells following PDT using AlS2Pc; mean values and standard deviations are reported, measured in arbitrary fluorescent units. Control cells: un-loaded irradiated DOK cells; AlS2Pc cells: loaded irradiated DOK cells.
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Fig4: Results of protein microarray analysis of the main proteins involved in apoptosis in DOK cells following PDT using AlS2Pc; mean values and standard deviations are reported, measured in arbitrary fluorescent units. Control cells: un-loaded irradiated DOK cells; AlS2Pc cells: loaded irradiated DOK cells.

Mentions: We have analyzed the apoptosis related biomolecules that showed the variations with the greatest amplitude (FigureĀ 4). Anti-apoptotic proteins Bcl-2 ("B-cell lymphoma 2") and Bcl-XL ("B-cell lymphoma 2 extra large") block apoptosis in usual conditions; their increase inhibits apoptosis, while their variation in the opposite direction facilitates programmed cell death[17]. This modulator role of Bcl-2 proteins in cell apoptosis triggered by PDT is still controversial. The overexpression of Bcl-2 could either be efficient or abstaining in tumor cells killing upon experimental PDT, depending on cell type and/or photosensitizer nature[18].Figure 4


Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy.

Matei C, Tampa M, Caruntu C, Ion RM, Georgescu SR, Dumitrascu GR, Constantin C, Neagu M - Biol. Res. (2014)

Results of protein microarray analysis of the main proteins involved in apoptosis in DOK cells following PDT using AlS2Pc; mean values and standard deviations are reported, measured in arbitrary fluorescent units. Control cells: un-loaded irradiated DOK cells; AlS2Pc cells: loaded irradiated DOK cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4125699&req=5

Fig4: Results of protein microarray analysis of the main proteins involved in apoptosis in DOK cells following PDT using AlS2Pc; mean values and standard deviations are reported, measured in arbitrary fluorescent units. Control cells: un-loaded irradiated DOK cells; AlS2Pc cells: loaded irradiated DOK cells.
Mentions: We have analyzed the apoptosis related biomolecules that showed the variations with the greatest amplitude (FigureĀ 4). Anti-apoptotic proteins Bcl-2 ("B-cell lymphoma 2") and Bcl-XL ("B-cell lymphoma 2 extra large") block apoptosis in usual conditions; their increase inhibits apoptosis, while their variation in the opposite direction facilitates programmed cell death[17]. This modulator role of Bcl-2 proteins in cell apoptosis triggered by PDT is still controversial. The overexpression of Bcl-2 could either be efficient or abstaining in tumor cells killing upon experimental PDT, depending on cell type and/or photosensitizer nature[18].Figure 4

Bottom Line: The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential.The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis.Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Photodynamic therapy is an alternative treatment of muco-cutaneous tumors that uses a light source able to photoactivate a chemical compound that acts as a photosensitizer. The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential.

Results: The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis. Using protein microarray we evaluate the possible molecular pathways by which photodynamic therapy activates apoptosis in dysplastic oral keratinocytes cells, leading to the tumoral cells destruction. Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude.

Conclusions: Up to date, the intimate molecular apoptotic mechanisms activated by photodynamic therapy with this type of phthalocyanine in dysplastic human oral keratinocytes are not completely elucidated. With protein microarray as high-throughput proteomic approach a better understanding of the manner in which photodynamic therapy leads to tumoral cell destruction can be obtained, by depicting apoptotic molecules that can be potentially triggered in future anti-tumoral therapies.

Show MeSH
Related in: MedlinePlus