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Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy.

Matei C, Tampa M, Caruntu C, Ion RM, Georgescu SR, Dumitrascu GR, Constantin C, Neagu M - Biol. Res. (2014)

Bottom Line: The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential.The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis.Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Photodynamic therapy is an alternative treatment of muco-cutaneous tumors that uses a light source able to photoactivate a chemical compound that acts as a photosensitizer. The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential.

Results: The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis. Using protein microarray we evaluate the possible molecular pathways by which photodynamic therapy activates apoptosis in dysplastic oral keratinocytes cells, leading to the tumoral cells destruction. Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude.

Conclusions: Up to date, the intimate molecular apoptotic mechanisms activated by photodynamic therapy with this type of phthalocyanine in dysplastic human oral keratinocytes are not completely elucidated. With protein microarray as high-throughput proteomic approach a better understanding of the manner in which photodynamic therapy leads to tumoral cell destruction can be obtained, by depicting apoptotic molecules that can be potentially triggered in future anti-tumoral therapies.

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Protein microarray technology principle.
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Fig2: Protein microarray technology principle.

Mentions: Protein microarray analysis has started to be increasingly used in various biomedical applications, but few papers are published using this technology for evaluating the complex apoptosis network triggered by PDT therapy. The basic principle and a schematic workflow for protein microarray technique are shown in FigureĀ 2. Recently published 5-Aminolevulinic acid (ALA) used to photosensitize mouse cerebral cortex took advantage of proteomic antibody microarrays. Using this method 112 proteins were detected with supposed epigenetic regulation potential. Among these proteins, the PDT using ALA precursor of porphyrin induced histone H3 dimethylation as well as up-regulation of protein Kaiso, a DNA methyl-binding protein, the overall effect being the suppression of the transcriptional activity in photosensitized cells. This recent study showed that protein microarray can furnish high-throughput results further to be used in therapeutical approaches. Moreover, this study showed that new therapeutical targets can be revealed upon assessment of epigenetic markers[7].Figure 2


Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy.

Matei C, Tampa M, Caruntu C, Ion RM, Georgescu SR, Dumitrascu GR, Constantin C, Neagu M - Biol. Res. (2014)

Protein microarray technology principle.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4125699&req=5

Fig2: Protein microarray technology principle.
Mentions: Protein microarray analysis has started to be increasingly used in various biomedical applications, but few papers are published using this technology for evaluating the complex apoptosis network triggered by PDT therapy. The basic principle and a schematic workflow for protein microarray technique are shown in FigureĀ 2. Recently published 5-Aminolevulinic acid (ALA) used to photosensitize mouse cerebral cortex took advantage of proteomic antibody microarrays. Using this method 112 proteins were detected with supposed epigenetic regulation potential. Among these proteins, the PDT using ALA precursor of porphyrin induced histone H3 dimethylation as well as up-regulation of protein Kaiso, a DNA methyl-binding protein, the overall effect being the suppression of the transcriptional activity in photosensitized cells. This recent study showed that protein microarray can furnish high-throughput results further to be used in therapeutical approaches. Moreover, this study showed that new therapeutical targets can be revealed upon assessment of epigenetic markers[7].Figure 2

Bottom Line: The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential.The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis.Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Photodynamic therapy is an alternative treatment of muco-cutaneous tumors that uses a light source able to photoactivate a chemical compound that acts as a photosensitizer. The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential.

Results: The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis. Using protein microarray we evaluate the possible molecular pathways by which photodynamic therapy activates apoptosis in dysplastic oral keratinocytes cells, leading to the tumoral cells destruction. Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude.

Conclusions: Up to date, the intimate molecular apoptotic mechanisms activated by photodynamic therapy with this type of phthalocyanine in dysplastic human oral keratinocytes are not completely elucidated. With protein microarray as high-throughput proteomic approach a better understanding of the manner in which photodynamic therapy leads to tumoral cell destruction can be obtained, by depicting apoptotic molecules that can be potentially triggered in future anti-tumoral therapies.

Show MeSH
Related in: MedlinePlus