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Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy.

Matei C, Tampa M, Caruntu C, Ion RM, Georgescu SR, Dumitrascu GR, Constantin C, Neagu M - Biol. Res. (2014)

Bottom Line: The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential.The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis.Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Photodynamic therapy is an alternative treatment of muco-cutaneous tumors that uses a light source able to photoactivate a chemical compound that acts as a photosensitizer. The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential.

Results: The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis. Using protein microarray we evaluate the possible molecular pathways by which photodynamic therapy activates apoptosis in dysplastic oral keratinocytes cells, leading to the tumoral cells destruction. Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude.

Conclusions: Up to date, the intimate molecular apoptotic mechanisms activated by photodynamic therapy with this type of phthalocyanine in dysplastic human oral keratinocytes are not completely elucidated. With protein microarray as high-throughput proteomic approach a better understanding of the manner in which photodynamic therapy leads to tumoral cell destruction can be obtained, by depicting apoptotic molecules that can be potentially triggered in future anti-tumoral therapies.

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The structure of metallo-sulphonated phthalocyanines (M = metal; R = SO3-/H).
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Related In: Results  -  Collection

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Fig1: The structure of metallo-sulphonated phthalocyanines (M = metal; R = SO3-/H).

Mentions: PDT uses an adequate light source able to photoactivate a chemical compound that acts like a photosensitiser; various photosensitizers are recently the subject of intense research worldwide, mainly porphyrin precursors and derivatives such as the phthalocyanines[3]. The latest are chemical structures sharing a four-ring pyrrol-like aromatic structure similar to a certain extent to that of the porphyrins; central inclusion of a metal ion such as Al, Zn, In or Ag modifies the photodynamic properties of the phthalocyanines (Figure 1). The central metal ion modulates the photodynamic properties by acting through the alteration of the electron density in the core of the compound, increasing π-electron delocalization and consequently changing the absorbance spectrum of the substance[4].Figure 1


Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy.

Matei C, Tampa M, Caruntu C, Ion RM, Georgescu SR, Dumitrascu GR, Constantin C, Neagu M - Biol. Res. (2014)

The structure of metallo-sulphonated phthalocyanines (M = metal; R = SO3-/H).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4125699&req=5

Fig1: The structure of metallo-sulphonated phthalocyanines (M = metal; R = SO3-/H).
Mentions: PDT uses an adequate light source able to photoactivate a chemical compound that acts like a photosensitiser; various photosensitizers are recently the subject of intense research worldwide, mainly porphyrin precursors and derivatives such as the phthalocyanines[3]. The latest are chemical structures sharing a four-ring pyrrol-like aromatic structure similar to a certain extent to that of the porphyrins; central inclusion of a metal ion such as Al, Zn, In or Ag modifies the photodynamic properties of the phthalocyanines (Figure 1). The central metal ion modulates the photodynamic properties by acting through the alteration of the electron density in the core of the compound, increasing π-electron delocalization and consequently changing the absorbance spectrum of the substance[4].Figure 1

Bottom Line: The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential.The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis.Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Photodynamic therapy is an alternative treatment of muco-cutaneous tumors that uses a light source able to photoactivate a chemical compound that acts as a photosensitizer. The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential.

Results: The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis. Using protein microarray we evaluate the possible molecular pathways by which photodynamic therapy activates apoptosis in dysplastic oral keratinocytes cells, leading to the tumoral cells destruction. Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude.

Conclusions: Up to date, the intimate molecular apoptotic mechanisms activated by photodynamic therapy with this type of phthalocyanine in dysplastic human oral keratinocytes are not completely elucidated. With protein microarray as high-throughput proteomic approach a better understanding of the manner in which photodynamic therapy leads to tumoral cell destruction can be obtained, by depicting apoptotic molecules that can be potentially triggered in future anti-tumoral therapies.

Show MeSH
Related in: MedlinePlus