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Weight loss herbal intervention therapy (W-LHIT) a non-appetite suppressing natural product controls weight and lowers cholesterol and glucose levels in a murine model.

Yang N, Chung D, Liu C, Liang B, Li XM - BMC Complement Altern Med (2014)

Bottom Line: In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues.Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. xiu-min.li@mssm.edu.

ABSTRACT

Background: The prevalence of obesity is increasing in industrialized countries. Obesity increases the risk of coronary artery disease, stroke, cancer, hypertension, and type-2 diabetes. Unfortunately, conventional obesity drug treatment is often associated with adverse effects. The objective of this study was to evaluate a novel natural formula, Weight loss herbal intervention therapy (W-LHIT), developed from traditional Chinese medicine, for weight control in a high-fat-diet (HFD) induced obesity murine model.

Methods: Two sets of experiments were performed. In experiment 1, 14-week-old C57BL/6 J male mice were fed with HFD for 21 days and then separated into 3 weight-matched groups. One group continued on the HFD as obese-controls. Two groups were switched from HFD to normal fat level diet (NFD) and sham or W-LHIT treated. In experiment 2, 25-week-old obese mice, following 2 weeks acclimatization, received either W-LHIT or sham treatment while maintained on HFD. In both sets of experiments, NFD fed, age matched normal weight mice served as normal controls. Body weight and food intake were recorded. Epididymal fat pad weight, serum glucose and cholesterol levels, as well as PPARγ and FABP4 gene expression in epididymal fat tissue were analyzed at the end of the experiment.

Results: In experiment 1, W-LHIT treated obese mice lost body weight 12.2 ± 3.8% whereas sham treated mice lost 5.5 ± 2.8% by day 10 after switching from the HFD to the NFD, without reduction of chow consumption. In experiment 2, W-LHIT treated obese mice maintained on the HFD had significantly lower body weight (8 fold less) than the sham treated mice. W-LHIT treatment also reduced epididymal fat pad weight, blood cholesterol and glucose levels versus sham treated mice without reduced chow consumption. In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues. Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.

Conclusions: W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

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Related in: MedlinePlus

Real time PCR of epididymal adipose tissues PPARγ(A) and FABP4 gene expressions (B) in experiment 2. Data expressed as Mean ± S.D. *p < 0.05 (n ≥ 4).
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Fig6: Real time PCR of epididymal adipose tissues PPARγ(A) and FABP4 gene expressions (B) in experiment 2. Data expressed as Mean ± S.D. *p < 0.05 (n ≥ 4).

Mentions: Total mRNA was extracted from epididymal fat tissues of sham treated, W-LHIT treated, and normal control mice in experiment 2, and PPARγ and FABP4 mRNA expression were analyzed using real time PCR. W-LHIT treatment significantly increased the expression of PPARγ (p < 0.05, Figure 6A) and FABP4 (p < 0.05, Figure 6B). We also analyzed CPT1, UCP2, and AMPK gene expression, which are related to fat oxidation and metabolism. The results showed that the relative gene expressions of CTP1, UCP2, and AMPK in W-LHIT treated mice also trended upward compared to that in sham treated mice (CTP1, 14.7 ± 12.6 vs. 1.14 ± 0.7; UCP2, 83.8 ± 82.6 vs. 2.2 ± 3.1; and AMPK, 19.1 ± 15.5 vs. 0.2 ± 0.3). However, these increases did not reach statistical significance.Figure 6


Weight loss herbal intervention therapy (W-LHIT) a non-appetite suppressing natural product controls weight and lowers cholesterol and glucose levels in a murine model.

Yang N, Chung D, Liu C, Liang B, Li XM - BMC Complement Altern Med (2014)

Real time PCR of epididymal adipose tissues PPARγ(A) and FABP4 gene expressions (B) in experiment 2. Data expressed as Mean ± S.D. *p < 0.05 (n ≥ 4).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4125697&req=5

Fig6: Real time PCR of epididymal adipose tissues PPARγ(A) and FABP4 gene expressions (B) in experiment 2. Data expressed as Mean ± S.D. *p < 0.05 (n ≥ 4).
Mentions: Total mRNA was extracted from epididymal fat tissues of sham treated, W-LHIT treated, and normal control mice in experiment 2, and PPARγ and FABP4 mRNA expression were analyzed using real time PCR. W-LHIT treatment significantly increased the expression of PPARγ (p < 0.05, Figure 6A) and FABP4 (p < 0.05, Figure 6B). We also analyzed CPT1, UCP2, and AMPK gene expression, which are related to fat oxidation and metabolism. The results showed that the relative gene expressions of CTP1, UCP2, and AMPK in W-LHIT treated mice also trended upward compared to that in sham treated mice (CTP1, 14.7 ± 12.6 vs. 1.14 ± 0.7; UCP2, 83.8 ± 82.6 vs. 2.2 ± 3.1; and AMPK, 19.1 ± 15.5 vs. 0.2 ± 0.3). However, these increases did not reach statistical significance.Figure 6

Bottom Line: In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues.Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. xiu-min.li@mssm.edu.

ABSTRACT

Background: The prevalence of obesity is increasing in industrialized countries. Obesity increases the risk of coronary artery disease, stroke, cancer, hypertension, and type-2 diabetes. Unfortunately, conventional obesity drug treatment is often associated with adverse effects. The objective of this study was to evaluate a novel natural formula, Weight loss herbal intervention therapy (W-LHIT), developed from traditional Chinese medicine, for weight control in a high-fat-diet (HFD) induced obesity murine model.

Methods: Two sets of experiments were performed. In experiment 1, 14-week-old C57BL/6 J male mice were fed with HFD for 21 days and then separated into 3 weight-matched groups. One group continued on the HFD as obese-controls. Two groups were switched from HFD to normal fat level diet (NFD) and sham or W-LHIT treated. In experiment 2, 25-week-old obese mice, following 2 weeks acclimatization, received either W-LHIT or sham treatment while maintained on HFD. In both sets of experiments, NFD fed, age matched normal weight mice served as normal controls. Body weight and food intake were recorded. Epididymal fat pad weight, serum glucose and cholesterol levels, as well as PPARγ and FABP4 gene expression in epididymal fat tissue were analyzed at the end of the experiment.

Results: In experiment 1, W-LHIT treated obese mice lost body weight 12.2 ± 3.8% whereas sham treated mice lost 5.5 ± 2.8% by day 10 after switching from the HFD to the NFD, without reduction of chow consumption. In experiment 2, W-LHIT treated obese mice maintained on the HFD had significantly lower body weight (8 fold less) than the sham treated mice. W-LHIT treatment also reduced epididymal fat pad weight, blood cholesterol and glucose levels versus sham treated mice without reduced chow consumption. In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues. Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.

Conclusions: W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

Show MeSH
Related in: MedlinePlus