Limits...
Weight loss herbal intervention therapy (W-LHIT) a non-appetite suppressing natural product controls weight and lowers cholesterol and glucose levels in a murine model.

Yang N, Chung D, Liu C, Liang B, Li XM - BMC Complement Altern Med (2014)

Bottom Line: In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues.Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. xiu-min.li@mssm.edu.

ABSTRACT

Background: The prevalence of obesity is increasing in industrialized countries. Obesity increases the risk of coronary artery disease, stroke, cancer, hypertension, and type-2 diabetes. Unfortunately, conventional obesity drug treatment is often associated with adverse effects. The objective of this study was to evaluate a novel natural formula, Weight loss herbal intervention therapy (W-LHIT), developed from traditional Chinese medicine, for weight control in a high-fat-diet (HFD) induced obesity murine model.

Methods: Two sets of experiments were performed. In experiment 1, 14-week-old C57BL/6 J male mice were fed with HFD for 21 days and then separated into 3 weight-matched groups. One group continued on the HFD as obese-controls. Two groups were switched from HFD to normal fat level diet (NFD) and sham or W-LHIT treated. In experiment 2, 25-week-old obese mice, following 2 weeks acclimatization, received either W-LHIT or sham treatment while maintained on HFD. In both sets of experiments, NFD fed, age matched normal weight mice served as normal controls. Body weight and food intake were recorded. Epididymal fat pad weight, serum glucose and cholesterol levels, as well as PPARγ and FABP4 gene expression in epididymal fat tissue were analyzed at the end of the experiment.

Results: In experiment 1, W-LHIT treated obese mice lost body weight 12.2 ± 3.8% whereas sham treated mice lost 5.5 ± 2.8% by day 10 after switching from the HFD to the NFD, without reduction of chow consumption. In experiment 2, W-LHIT treated obese mice maintained on the HFD had significantly lower body weight (8 fold less) than the sham treated mice. W-LHIT treatment also reduced epididymal fat pad weight, blood cholesterol and glucose levels versus sham treated mice without reduced chow consumption. In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues. Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.

Conclusions: W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

Show MeSH

Related in: MedlinePlus

Effect of W-LHIT on blood cholesterol and glucose levels of older obese mice in experiment 2. A. Cholesterol levels and B. Glucose levels in W-LHIT treated and sham-treated older obese mice and normal controls. Data are expressed as Mean ± S.D. *p < 0.05; **p < 0.01 (n ≥ 4).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4125697&req=5

Fig5: Effect of W-LHIT on blood cholesterol and glucose levels of older obese mice in experiment 2. A. Cholesterol levels and B. Glucose levels in W-LHIT treated and sham-treated older obese mice and normal controls. Data are expressed as Mean ± S.D. *p < 0.05; **p < 0.01 (n ≥ 4).

Mentions: In experiment 2, after 30-day treatment, mice were fasted overnight, and blood samples were collected. Serum cholesterol and glucose levels were measured. Mice in OB/HFD/Sham group showed significantly increased serum total cholesterol levels compared with NFD normal controls (195.0 ± 26.8 vs 93 ± 11 mg/dL, p < 0.01 Figure 5A). Cholesterol levels in OB/HFD/W-LHIT group (128.5 ± 37.6 mg/dL) were significantly lower than those of sham treated mice (p < 0.05) and were not statistically different from the NFD normal controls (Figure 5A).Figure 5


Weight loss herbal intervention therapy (W-LHIT) a non-appetite suppressing natural product controls weight and lowers cholesterol and glucose levels in a murine model.

Yang N, Chung D, Liu C, Liang B, Li XM - BMC Complement Altern Med (2014)

Effect of W-LHIT on blood cholesterol and glucose levels of older obese mice in experiment 2. A. Cholesterol levels and B. Glucose levels in W-LHIT treated and sham-treated older obese mice and normal controls. Data are expressed as Mean ± S.D. *p < 0.05; **p < 0.01 (n ≥ 4).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4125697&req=5

Fig5: Effect of W-LHIT on blood cholesterol and glucose levels of older obese mice in experiment 2. A. Cholesterol levels and B. Glucose levels in W-LHIT treated and sham-treated older obese mice and normal controls. Data are expressed as Mean ± S.D. *p < 0.05; **p < 0.01 (n ≥ 4).
Mentions: In experiment 2, after 30-day treatment, mice were fasted overnight, and blood samples were collected. Serum cholesterol and glucose levels were measured. Mice in OB/HFD/Sham group showed significantly increased serum total cholesterol levels compared with NFD normal controls (195.0 ± 26.8 vs 93 ± 11 mg/dL, p < 0.01 Figure 5A). Cholesterol levels in OB/HFD/W-LHIT group (128.5 ± 37.6 mg/dL) were significantly lower than those of sham treated mice (p < 0.05) and were not statistically different from the NFD normal controls (Figure 5A).Figure 5

Bottom Line: In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues.Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. xiu-min.li@mssm.edu.

ABSTRACT

Background: The prevalence of obesity is increasing in industrialized countries. Obesity increases the risk of coronary artery disease, stroke, cancer, hypertension, and type-2 diabetes. Unfortunately, conventional obesity drug treatment is often associated with adverse effects. The objective of this study was to evaluate a novel natural formula, Weight loss herbal intervention therapy (W-LHIT), developed from traditional Chinese medicine, for weight control in a high-fat-diet (HFD) induced obesity murine model.

Methods: Two sets of experiments were performed. In experiment 1, 14-week-old C57BL/6 J male mice were fed with HFD for 21 days and then separated into 3 weight-matched groups. One group continued on the HFD as obese-controls. Two groups were switched from HFD to normal fat level diet (NFD) and sham or W-LHIT treated. In experiment 2, 25-week-old obese mice, following 2 weeks acclimatization, received either W-LHIT or sham treatment while maintained on HFD. In both sets of experiments, NFD fed, age matched normal weight mice served as normal controls. Body weight and food intake were recorded. Epididymal fat pad weight, serum glucose and cholesterol levels, as well as PPARγ and FABP4 gene expression in epididymal fat tissue were analyzed at the end of the experiment.

Results: In experiment 1, W-LHIT treated obese mice lost body weight 12.2 ± 3.8% whereas sham treated mice lost 5.5 ± 2.8% by day 10 after switching from the HFD to the NFD, without reduction of chow consumption. In experiment 2, W-LHIT treated obese mice maintained on the HFD had significantly lower body weight (8 fold less) than the sham treated mice. W-LHIT treatment also reduced epididymal fat pad weight, blood cholesterol and glucose levels versus sham treated mice without reduced chow consumption. In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues. Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.

Conclusions: W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

Show MeSH
Related in: MedlinePlus