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Weight loss herbal intervention therapy (W-LHIT) a non-appetite suppressing natural product controls weight and lowers cholesterol and glucose levels in a murine model.

Yang N, Chung D, Liu C, Liang B, Li XM - BMC Complement Altern Med (2014)

Bottom Line: In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues.Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. xiu-min.li@mssm.edu.

ABSTRACT

Background: The prevalence of obesity is increasing in industrialized countries. Obesity increases the risk of coronary artery disease, stroke, cancer, hypertension, and type-2 diabetes. Unfortunately, conventional obesity drug treatment is often associated with adverse effects. The objective of this study was to evaluate a novel natural formula, Weight loss herbal intervention therapy (W-LHIT), developed from traditional Chinese medicine, for weight control in a high-fat-diet (HFD) induced obesity murine model.

Methods: Two sets of experiments were performed. In experiment 1, 14-week-old C57BL/6 J male mice were fed with HFD for 21 days and then separated into 3 weight-matched groups. One group continued on the HFD as obese-controls. Two groups were switched from HFD to normal fat level diet (NFD) and sham or W-LHIT treated. In experiment 2, 25-week-old obese mice, following 2 weeks acclimatization, received either W-LHIT or sham treatment while maintained on HFD. In both sets of experiments, NFD fed, age matched normal weight mice served as normal controls. Body weight and food intake were recorded. Epididymal fat pad weight, serum glucose and cholesterol levels, as well as PPARγ and FABP4 gene expression in epididymal fat tissue were analyzed at the end of the experiment.

Results: In experiment 1, W-LHIT treated obese mice lost body weight 12.2 ± 3.8% whereas sham treated mice lost 5.5 ± 2.8% by day 10 after switching from the HFD to the NFD, without reduction of chow consumption. In experiment 2, W-LHIT treated obese mice maintained on the HFD had significantly lower body weight (8 fold less) than the sham treated mice. W-LHIT treatment also reduced epididymal fat pad weight, blood cholesterol and glucose levels versus sham treated mice without reduced chow consumption. In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues. Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.

Conclusions: W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

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Related in: MedlinePlus

Effect of W-LHIT on body weights of older obese mice in experiment 2. A. Protocols of weight loss experiment 2; B. Average body-weight change curve of sham and W-LHIT treated obese mice; C. Daily body weight change before and after treatment; D. Daily food consumption per mouse; ***p < 0.001(n = 5). Data represent two independent experiments.
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Fig3: Effect of W-LHIT on body weights of older obese mice in experiment 2. A. Protocols of weight loss experiment 2; B. Average body-weight change curve of sham and W-LHIT treated obese mice; C. Daily body weight change before and after treatment; D. Daily food consumption per mouse; ***p < 0.001(n = 5). Data represent two independent experiments.

Mentions: In experiment 2, to determine the effect of W-LHIT on HFD-induced weight gain in older mice, 14 week-old mice were maintained in an animal facility at Icahn School of Medicine at Mount Sinai for 9 weeks on HFD until 23 weeks old (roughly equivalent to 40 human years). They were then subjected to 2 week acclimatization by i.g. water administration. These 25 week-old obese mice were divided into 2 weight matched groups (Figure 3A). Group 1 mice continued on HFD and sham treatment as obese controls (OB/HFD/Sham) while group 2 mice continued on HFD and received W-LHIT treatment (OB/HFD/W-LHIT). Treatment duration was 30 days. Normal weight mice fed with NFD and water sham treatment were used as normal controls (Normal/NFD/Sham).Figure 3


Weight loss herbal intervention therapy (W-LHIT) a non-appetite suppressing natural product controls weight and lowers cholesterol and glucose levels in a murine model.

Yang N, Chung D, Liu C, Liang B, Li XM - BMC Complement Altern Med (2014)

Effect of W-LHIT on body weights of older obese mice in experiment 2. A. Protocols of weight loss experiment 2; B. Average body-weight change curve of sham and W-LHIT treated obese mice; C. Daily body weight change before and after treatment; D. Daily food consumption per mouse; ***p < 0.001(n = 5). Data represent two independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4125697&req=5

Fig3: Effect of W-LHIT on body weights of older obese mice in experiment 2. A. Protocols of weight loss experiment 2; B. Average body-weight change curve of sham and W-LHIT treated obese mice; C. Daily body weight change before and after treatment; D. Daily food consumption per mouse; ***p < 0.001(n = 5). Data represent two independent experiments.
Mentions: In experiment 2, to determine the effect of W-LHIT on HFD-induced weight gain in older mice, 14 week-old mice were maintained in an animal facility at Icahn School of Medicine at Mount Sinai for 9 weeks on HFD until 23 weeks old (roughly equivalent to 40 human years). They were then subjected to 2 week acclimatization by i.g. water administration. These 25 week-old obese mice were divided into 2 weight matched groups (Figure 3A). Group 1 mice continued on HFD and sham treatment as obese controls (OB/HFD/Sham) while group 2 mice continued on HFD and received W-LHIT treatment (OB/HFD/W-LHIT). Treatment duration was 30 days. Normal weight mice fed with NFD and water sham treatment were used as normal controls (Normal/NFD/Sham).Figure 3

Bottom Line: In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues.Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. xiu-min.li@mssm.edu.

ABSTRACT

Background: The prevalence of obesity is increasing in industrialized countries. Obesity increases the risk of coronary artery disease, stroke, cancer, hypertension, and type-2 diabetes. Unfortunately, conventional obesity drug treatment is often associated with adverse effects. The objective of this study was to evaluate a novel natural formula, Weight loss herbal intervention therapy (W-LHIT), developed from traditional Chinese medicine, for weight control in a high-fat-diet (HFD) induced obesity murine model.

Methods: Two sets of experiments were performed. In experiment 1, 14-week-old C57BL/6 J male mice were fed with HFD for 21 days and then separated into 3 weight-matched groups. One group continued on the HFD as obese-controls. Two groups were switched from HFD to normal fat level diet (NFD) and sham or W-LHIT treated. In experiment 2, 25-week-old obese mice, following 2 weeks acclimatization, received either W-LHIT or sham treatment while maintained on HFD. In both sets of experiments, NFD fed, age matched normal weight mice served as normal controls. Body weight and food intake were recorded. Epididymal fat pad weight, serum glucose and cholesterol levels, as well as PPARγ and FABP4 gene expression in epididymal fat tissue were analyzed at the end of the experiment.

Results: In experiment 1, W-LHIT treated obese mice lost body weight 12.2 ± 3.8% whereas sham treated mice lost 5.5 ± 2.8% by day 10 after switching from the HFD to the NFD, without reduction of chow consumption. In experiment 2, W-LHIT treated obese mice maintained on the HFD had significantly lower body weight (8 fold less) than the sham treated mice. W-LHIT treatment also reduced epididymal fat pad weight, blood cholesterol and glucose levels versus sham treated mice without reduced chow consumption. In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues. Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.

Conclusions: W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

Show MeSH
Related in: MedlinePlus