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Weight loss herbal intervention therapy (W-LHIT) a non-appetite suppressing natural product controls weight and lowers cholesterol and glucose levels in a murine model.

Yang N, Chung D, Liu C, Liang B, Li XM - BMC Complement Altern Med (2014)

Bottom Line: In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues.Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. xiu-min.li@mssm.edu.

ABSTRACT

Background: The prevalence of obesity is increasing in industrialized countries. Obesity increases the risk of coronary artery disease, stroke, cancer, hypertension, and type-2 diabetes. Unfortunately, conventional obesity drug treatment is often associated with adverse effects. The objective of this study was to evaluate a novel natural formula, Weight loss herbal intervention therapy (W-LHIT), developed from traditional Chinese medicine, for weight control in a high-fat-diet (HFD) induced obesity murine model.

Methods: Two sets of experiments were performed. In experiment 1, 14-week-old C57BL/6 J male mice were fed with HFD for 21 days and then separated into 3 weight-matched groups. One group continued on the HFD as obese-controls. Two groups were switched from HFD to normal fat level diet (NFD) and sham or W-LHIT treated. In experiment 2, 25-week-old obese mice, following 2 weeks acclimatization, received either W-LHIT or sham treatment while maintained on HFD. In both sets of experiments, NFD fed, age matched normal weight mice served as normal controls. Body weight and food intake were recorded. Epididymal fat pad weight, serum glucose and cholesterol levels, as well as PPARγ and FABP4 gene expression in epididymal fat tissue were analyzed at the end of the experiment.

Results: In experiment 1, W-LHIT treated obese mice lost body weight 12.2 ± 3.8% whereas sham treated mice lost 5.5 ± 2.8% by day 10 after switching from the HFD to the NFD, without reduction of chow consumption. In experiment 2, W-LHIT treated obese mice maintained on the HFD had significantly lower body weight (8 fold less) than the sham treated mice. W-LHIT treatment also reduced epididymal fat pad weight, blood cholesterol and glucose levels versus sham treated mice without reduced chow consumption. In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues. Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.

Conclusions: W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

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Related in: MedlinePlus

Effect of W-LHIT on young obese mice bodyweights in experiment 1. A. Protocols of weight loss experiment 1; B. Average body-weight change curve of sham and W-LHIT treated obese mice over time; C. Daily body weight change; D. Daily food consumption per mouse; **p < 0.01; ***p < 0.001 (n = 5). Data represent two independent experiments.
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Fig2: Effect of W-LHIT on young obese mice bodyweights in experiment 1. A. Protocols of weight loss experiment 1; B. Average body-weight change curve of sham and W-LHIT treated obese mice over time; C. Daily body weight change; D. Daily food consumption per mouse; **p < 0.01; ***p < 0.001 (n = 5). Data represent two independent experiments.

Mentions: Each mouse received 84 mg W-LHIT daily, dissolved in 1.0 mL drinking water, and intragastrically (i.g.) administered by two separate feedings (0.5 mL per feeding 4 hours apart using a standard mouse feeding needle (VWR, Radnor, PA). The W-LHIT dose was determined by a conversion table of equivalent human to animal dose [25]. We employed two protocols in two sets of experiments to determine the effect W-LHIT on weight control as follows: The first set of experiments was designed to determine the effect of W-LHIT on weight loss as added-on therapy to dietary calorie reduction on young mice. In this set of experiments, three groups of age matched 14 week-old mice (equivalent to human age of 19 years) were first sham treated by i.g. administration of water while continuing on the HFD for 3 weeks. This protocol was used to acclimatize mice to i.g. administration to prevent potential gavage procedure effect on weight changes (run-in period). Sham treated normal weight mice (G4) fed a NFD served as normal controls. Three weeks later, all mice were weighed. Group 1 obese mice continued on HFD and sham treatment as the obesity control group (OB/HFD/Sham). Both group 2 and 3 obese mice were switched from HFD to NFD, but group 2 mice received W-LHIT (OB/NFD/W-LHIT) whereas group 3 mice received water sham treatment (OB/NFD/Sham). Group 4, the normal weight mice, continued on NFD and water sham treatment to serve as normal controls (Normal/NFD/Sham). Treatment duration was 10 days (Figure 2A).Figure 2


Weight loss herbal intervention therapy (W-LHIT) a non-appetite suppressing natural product controls weight and lowers cholesterol and glucose levels in a murine model.

Yang N, Chung D, Liu C, Liang B, Li XM - BMC Complement Altern Med (2014)

Effect of W-LHIT on young obese mice bodyweights in experiment 1. A. Protocols of weight loss experiment 1; B. Average body-weight change curve of sham and W-LHIT treated obese mice over time; C. Daily body weight change; D. Daily food consumption per mouse; **p < 0.01; ***p < 0.001 (n = 5). Data represent two independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4125697&req=5

Fig2: Effect of W-LHIT on young obese mice bodyweights in experiment 1. A. Protocols of weight loss experiment 1; B. Average body-weight change curve of sham and W-LHIT treated obese mice over time; C. Daily body weight change; D. Daily food consumption per mouse; **p < 0.01; ***p < 0.001 (n = 5). Data represent two independent experiments.
Mentions: Each mouse received 84 mg W-LHIT daily, dissolved in 1.0 mL drinking water, and intragastrically (i.g.) administered by two separate feedings (0.5 mL per feeding 4 hours apart using a standard mouse feeding needle (VWR, Radnor, PA). The W-LHIT dose was determined by a conversion table of equivalent human to animal dose [25]. We employed two protocols in two sets of experiments to determine the effect W-LHIT on weight control as follows: The first set of experiments was designed to determine the effect of W-LHIT on weight loss as added-on therapy to dietary calorie reduction on young mice. In this set of experiments, three groups of age matched 14 week-old mice (equivalent to human age of 19 years) were first sham treated by i.g. administration of water while continuing on the HFD for 3 weeks. This protocol was used to acclimatize mice to i.g. administration to prevent potential gavage procedure effect on weight changes (run-in period). Sham treated normal weight mice (G4) fed a NFD served as normal controls. Three weeks later, all mice were weighed. Group 1 obese mice continued on HFD and sham treatment as the obesity control group (OB/HFD/Sham). Both group 2 and 3 obese mice were switched from HFD to NFD, but group 2 mice received W-LHIT (OB/NFD/W-LHIT) whereas group 3 mice received water sham treatment (OB/NFD/Sham). Group 4, the normal weight mice, continued on NFD and water sham treatment to serve as normal controls (Normal/NFD/Sham). Treatment duration was 10 days (Figure 2A).Figure 2

Bottom Line: In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues.Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. xiu-min.li@mssm.edu.

ABSTRACT

Background: The prevalence of obesity is increasing in industrialized countries. Obesity increases the risk of coronary artery disease, stroke, cancer, hypertension, and type-2 diabetes. Unfortunately, conventional obesity drug treatment is often associated with adverse effects. The objective of this study was to evaluate a novel natural formula, Weight loss herbal intervention therapy (W-LHIT), developed from traditional Chinese medicine, for weight control in a high-fat-diet (HFD) induced obesity murine model.

Methods: Two sets of experiments were performed. In experiment 1, 14-week-old C57BL/6 J male mice were fed with HFD for 21 days and then separated into 3 weight-matched groups. One group continued on the HFD as obese-controls. Two groups were switched from HFD to normal fat level diet (NFD) and sham or W-LHIT treated. In experiment 2, 25-week-old obese mice, following 2 weeks acclimatization, received either W-LHIT or sham treatment while maintained on HFD. In both sets of experiments, NFD fed, age matched normal weight mice served as normal controls. Body weight and food intake were recorded. Epididymal fat pad weight, serum glucose and cholesterol levels, as well as PPARγ and FABP4 gene expression in epididymal fat tissue were analyzed at the end of the experiment.

Results: In experiment 1, W-LHIT treated obese mice lost body weight 12.2 ± 3.8% whereas sham treated mice lost 5.5 ± 2.8% by day 10 after switching from the HFD to the NFD, without reduction of chow consumption. In experiment 2, W-LHIT treated obese mice maintained on the HFD had significantly lower body weight (8 fold less) than the sham treated mice. W-LHIT treatment also reduced epididymal fat pad weight, blood cholesterol and glucose levels versus sham treated mice without reduced chow consumption. In addition, significantly increased PPARγ (peroxisome proliferator activated receptor γ) and FABP4 (fatty acid binding protein 4) gene expression were found in epdidymal fat tissues. Liver and kidney function and hematology testing results of W-LHIT treated mice were within the normal range.

Conclusions: W-LHIT significantly and safely reduced body weight, normalized glucose and cholesterol levels in obese mice, without suppression of appetite, and increased adipocyte PPARγ and FABP4 gene expression.

Show MeSH
Related in: MedlinePlus