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Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka.

Gajapathy K, Jude PJ, Goodacre SL, Peiris LB, Ramasamy R, Surendran SN - Parasit Vectors (2014)

Bottom Line: DNA was extracted from morphologically identified An. barbirostris specimens from Sri Lanka, the COI and ITS2 regions amplified and their sequences analysed by comparison with other GenBank entries.Maximum likelihood trees suggested that Sri Lankan An. barbirostris constitute a different molecular type most closely related to clade I.Considering the uncorrected p distances between the clade I and Sri Lankan specimens it is fair to assume that the specimens collected from widely separated locations in Sri Lanka with morphology characteristic of An. barbirostris s.l. form a new molecular type with close resemblance to An. barbirostris s.s from Indonesia and Thailand.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, University of Jaffna, Jaffna 40000, Sri Lanka. gayan156@gmail.com.

ABSTRACT

Background: Anopheles barbirostris is a vector of malaria in Sri Lanka. The taxon exists as a species complex in the Southeast Asian region. Previous studies using molecular markers suggest that there are more than 4 distinct clades within the An. barbirostris complex in Southeast Asia. The present study characterizes Sri Lankan An. barbirostris using mtDNA cytochrome oxidase subunit I (COI) and ribosomal RNA internal transcribed spacer 2 (ITS2) gene sequences.

Findings: DNA was extracted from morphologically identified An. barbirostris specimens from Sri Lanka, the COI and ITS2 regions amplified and their sequences analysed by comparison with other GenBank entries. Maximum likelihood trees suggested that Sri Lankan An. barbirostris constitute a different molecular type most closely related to clade I.

Conclusions: Considering the uncorrected p distances between the clade I and Sri Lankan specimens it is fair to assume that the specimens collected from widely separated locations in Sri Lanka with morphology characteristic of An. barbirostris s.l. form a new molecular type with close resemblance to An. barbirostris s.s from Indonesia and Thailand.

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Related in: MedlinePlus

Maximum Likelihood tree created from CO1 sequence data (400 bp) using Phyml[15]with the HKY + G + I substitution model. The nodes are shown with their bootstrap values; An. coustani was used as the out-group. The Genbank accession numbers for Sri Lankan samples are KJ580501 - KJ580505.
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Fig2: Maximum Likelihood tree created from CO1 sequence data (400 bp) using Phyml[15]with the HKY + G + I substitution model. The nodes are shown with their bootstrap values; An. coustani was used as the out-group. The Genbank accession numbers for Sri Lankan samples are KJ580501 - KJ580505.

Mentions: All the chromatograms were clean and the ambiguous ends were removed before sequence analysis. The phylogenetic tree (Figure 2) constructed with 400 bp of COI sequence data, including those from previously published sequences, confirmed that all five sequenced Sri Lankan specimens were members of the Barbirostris Complex. Sri Lankan specimens formed a sister clade to clade I of Paredes-Esquivel et al. [6], which is An. barbirostris s.s. Compared to sequences from the most closely related clade I, the Sri Lankan specimens showed 5.1% variation (with 20 variable sites) in the COI sequences. All the mutations were silent except two where tyrosine was replaced by serine and alanine replaced by proline. Intra-specific variations (0.4%), not associated with specific location, were observed among the Sri Lankan COI sequences.


Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka.

Gajapathy K, Jude PJ, Goodacre SL, Peiris LB, Ramasamy R, Surendran SN - Parasit Vectors (2014)

Maximum Likelihood tree created from CO1 sequence data (400 bp) using Phyml[15]with the HKY + G + I substitution model. The nodes are shown with their bootstrap values; An. coustani was used as the out-group. The Genbank accession numbers for Sri Lankan samples are KJ580501 - KJ580505.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4125690&req=5

Fig2: Maximum Likelihood tree created from CO1 sequence data (400 bp) using Phyml[15]with the HKY + G + I substitution model. The nodes are shown with their bootstrap values; An. coustani was used as the out-group. The Genbank accession numbers for Sri Lankan samples are KJ580501 - KJ580505.
Mentions: All the chromatograms were clean and the ambiguous ends were removed before sequence analysis. The phylogenetic tree (Figure 2) constructed with 400 bp of COI sequence data, including those from previously published sequences, confirmed that all five sequenced Sri Lankan specimens were members of the Barbirostris Complex. Sri Lankan specimens formed a sister clade to clade I of Paredes-Esquivel et al. [6], which is An. barbirostris s.s. Compared to sequences from the most closely related clade I, the Sri Lankan specimens showed 5.1% variation (with 20 variable sites) in the COI sequences. All the mutations were silent except two where tyrosine was replaced by serine and alanine replaced by proline. Intra-specific variations (0.4%), not associated with specific location, were observed among the Sri Lankan COI sequences.

Bottom Line: DNA was extracted from morphologically identified An. barbirostris specimens from Sri Lanka, the COI and ITS2 regions amplified and their sequences analysed by comparison with other GenBank entries.Maximum likelihood trees suggested that Sri Lankan An. barbirostris constitute a different molecular type most closely related to clade I.Considering the uncorrected p distances between the clade I and Sri Lankan specimens it is fair to assume that the specimens collected from widely separated locations in Sri Lanka with morphology characteristic of An. barbirostris s.l. form a new molecular type with close resemblance to An. barbirostris s.s from Indonesia and Thailand.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, University of Jaffna, Jaffna 40000, Sri Lanka. gayan156@gmail.com.

ABSTRACT

Background: Anopheles barbirostris is a vector of malaria in Sri Lanka. The taxon exists as a species complex in the Southeast Asian region. Previous studies using molecular markers suggest that there are more than 4 distinct clades within the An. barbirostris complex in Southeast Asia. The present study characterizes Sri Lankan An. barbirostris using mtDNA cytochrome oxidase subunit I (COI) and ribosomal RNA internal transcribed spacer 2 (ITS2) gene sequences.

Findings: DNA was extracted from morphologically identified An. barbirostris specimens from Sri Lanka, the COI and ITS2 regions amplified and their sequences analysed by comparison with other GenBank entries. Maximum likelihood trees suggested that Sri Lankan An. barbirostris constitute a different molecular type most closely related to clade I.

Conclusions: Considering the uncorrected p distances between the clade I and Sri Lankan specimens it is fair to assume that the specimens collected from widely separated locations in Sri Lanka with morphology characteristic of An. barbirostris s.l. form a new molecular type with close resemblance to An. barbirostris s.s from Indonesia and Thailand.

Show MeSH
Related in: MedlinePlus