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Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka.

Gajapathy K, Jude PJ, Goodacre SL, Peiris LB, Ramasamy R, Surendran SN - Parasit Vectors (2014)

Bottom Line: DNA was extracted from morphologically identified An. barbirostris specimens from Sri Lanka, the COI and ITS2 regions amplified and their sequences analysed by comparison with other GenBank entries.Maximum likelihood trees suggested that Sri Lankan An. barbirostris constitute a different molecular type most closely related to clade I.Considering the uncorrected p distances between the clade I and Sri Lankan specimens it is fair to assume that the specimens collected from widely separated locations in Sri Lanka with morphology characteristic of An. barbirostris s.l. form a new molecular type with close resemblance to An. barbirostris s.s from Indonesia and Thailand.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, University of Jaffna, Jaffna 40000, Sri Lanka. gayan156@gmail.com.

ABSTRACT

Background: Anopheles barbirostris is a vector of malaria in Sri Lanka. The taxon exists as a species complex in the Southeast Asian region. Previous studies using molecular markers suggest that there are more than 4 distinct clades within the An. barbirostris complex in Southeast Asia. The present study characterizes Sri Lankan An. barbirostris using mtDNA cytochrome oxidase subunit I (COI) and ribosomal RNA internal transcribed spacer 2 (ITS2) gene sequences.

Findings: DNA was extracted from morphologically identified An. barbirostris specimens from Sri Lanka, the COI and ITS2 regions amplified and their sequences analysed by comparison with other GenBank entries. Maximum likelihood trees suggested that Sri Lankan An. barbirostris constitute a different molecular type most closely related to clade I.

Conclusions: Considering the uncorrected p distances between the clade I and Sri Lankan specimens it is fair to assume that the specimens collected from widely separated locations in Sri Lanka with morphology characteristic of An. barbirostris s.l. form a new molecular type with close resemblance to An. barbirostris s.s from Indonesia and Thailand.

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Map of Sri Lanka showingAnopheles barbirostriscollection sites.
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Fig1: Map of Sri Lanka showingAnopheles barbirostriscollection sites.

Mentions: Anopheline mosquitoes were collected from the widely separated locations of Hambantota, Nainadeevu and Puttalam in Sri Lanka (FigureĀ 1) from January to July, 2012 using cattle baited traps. Mosquitoes were identified based on their morphology using taxonomic keys created for Sri Lankan anopheline mosquitoes [12].Figure 1


Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka.

Gajapathy K, Jude PJ, Goodacre SL, Peiris LB, Ramasamy R, Surendran SN - Parasit Vectors (2014)

Map of Sri Lanka showingAnopheles barbirostriscollection sites.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4125690&req=5

Fig1: Map of Sri Lanka showingAnopheles barbirostriscollection sites.
Mentions: Anopheline mosquitoes were collected from the widely separated locations of Hambantota, Nainadeevu and Puttalam in Sri Lanka (FigureĀ 1) from January to July, 2012 using cattle baited traps. Mosquitoes were identified based on their morphology using taxonomic keys created for Sri Lankan anopheline mosquitoes [12].Figure 1

Bottom Line: DNA was extracted from morphologically identified An. barbirostris specimens from Sri Lanka, the COI and ITS2 regions amplified and their sequences analysed by comparison with other GenBank entries.Maximum likelihood trees suggested that Sri Lankan An. barbirostris constitute a different molecular type most closely related to clade I.Considering the uncorrected p distances between the clade I and Sri Lankan specimens it is fair to assume that the specimens collected from widely separated locations in Sri Lanka with morphology characteristic of An. barbirostris s.l. form a new molecular type with close resemblance to An. barbirostris s.s from Indonesia and Thailand.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, University of Jaffna, Jaffna 40000, Sri Lanka. gayan156@gmail.com.

ABSTRACT

Background: Anopheles barbirostris is a vector of malaria in Sri Lanka. The taxon exists as a species complex in the Southeast Asian region. Previous studies using molecular markers suggest that there are more than 4 distinct clades within the An. barbirostris complex in Southeast Asia. The present study characterizes Sri Lankan An. barbirostris using mtDNA cytochrome oxidase subunit I (COI) and ribosomal RNA internal transcribed spacer 2 (ITS2) gene sequences.

Findings: DNA was extracted from morphologically identified An. barbirostris specimens from Sri Lanka, the COI and ITS2 regions amplified and their sequences analysed by comparison with other GenBank entries. Maximum likelihood trees suggested that Sri Lankan An. barbirostris constitute a different molecular type most closely related to clade I.

Conclusions: Considering the uncorrected p distances between the clade I and Sri Lankan specimens it is fair to assume that the specimens collected from widely separated locations in Sri Lanka with morphology characteristic of An. barbirostris s.l. form a new molecular type with close resemblance to An. barbirostris s.s from Indonesia and Thailand.

Show MeSH
Related in: MedlinePlus