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Habitual exercise plus dietary supplementation with milk fat globule membrane improves muscle function deficits via neuromuscular development in senescence-accelerated mice.

Haramizu S, Mori T, Yano M, Ota N, Hashizume K, Otsuka A, Hase T, Shimotoyodome A - Springerplus (2014)

Bottom Line: Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively).Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression.Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.

ABSTRACT
We examined the effects of habitual exercise plus nutritional intervention through consumption of milk fat globule membrane (MFGM), a milk component, on aging-related deficits in muscle mass and function in senescence-accelerated P1 mice. Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively). Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression. Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression. These findings suggest that habitual exercise plus dietary MFGM improves muscle function deficits through neuromuscular development, and that phospholipid and sphingolipid in MFGM contribute to its physiological actions.

No MeSH data available.


Effects of milk-fat globule membrane (MFGM), the phospholipid fraction (PLF), the sphingolipid fraction (SLF), and sphingomyelin (SPM), plus mechanical stretching, on expression of the mRNAs of Dok-7, MuSK, and myogenin in differentiating C2C12 cells. Levels of expression of the mRNAs of Dok-7 (A), MuSK (B), and myogenin (C) were measured by using quantitative real-time PCR. Expression of each gene was normalized against that of the housekeeping gene encoding ribosomal protein, large, P0 (RPLP0/36B4). Values are means ± S.E. of 6 samples. *P < 0.05, significant difference vs. control group by Dunnett’s posthoc test. Values are expressed as ratios, using the value of the control group as 1.0.
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Fig5: Effects of milk-fat globule membrane (MFGM), the phospholipid fraction (PLF), the sphingolipid fraction (SLF), and sphingomyelin (SPM), plus mechanical stretching, on expression of the mRNAs of Dok-7, MuSK, and myogenin in differentiating C2C12 cells. Levels of expression of the mRNAs of Dok-7 (A), MuSK (B), and myogenin (C) were measured by using quantitative real-time PCR. Expression of each gene was normalized against that of the housekeeping gene encoding ribosomal protein, large, P0 (RPLP0/36B4). Values are means ± S.E. of 6 samples. *P < 0.05, significant difference vs. control group by Dunnett’s posthoc test. Values are expressed as ratios, using the value of the control group as 1.0.

Mentions: Skeletal muscle is highly adaptable and responds to exercise and training, thus increasing muscle mass and function. To further understand the mechanisms underlying the adaptive response of skeletal muscles in vivo, in vitro culture systems that use mechanical stretching of cultured myotubes have been developed to mimic in vivo muscle physiology (Passey et al. 2011), including neuromuscular adaptation in response to exercise (Folland and Williams 2007; Hubatsch and Jasmin 1997; Jasmin et al. 1991; Sveistrup et al. 1995). Accordingly, we examined the effects of MFGM-derived fractions and sphingomyelin combined with mechanical stretch (as a substitute for exercise in vivo) on the expression of several genes.Treatment of the stretched cells with MFGM, PLF, SLF, or sphingomyelin significantly increased Dok-7 gene expression compared with that in cells that received mechanical stretching alone; this was evident especially in the cells treated with SLF or sphingomyelin (Figure 5). Myogenin gene expression was increased significantly by treatment with 0.01% MFGM or with 0.001% or 0.005% PLF fraction plus mechanical stretching of the cells. These results are consistent with the effects of the combination of habitual exercise and dietary MFGM observed in vivo.Figure 5


Habitual exercise plus dietary supplementation with milk fat globule membrane improves muscle function deficits via neuromuscular development in senescence-accelerated mice.

Haramizu S, Mori T, Yano M, Ota N, Hashizume K, Otsuka A, Hase T, Shimotoyodome A - Springerplus (2014)

Effects of milk-fat globule membrane (MFGM), the phospholipid fraction (PLF), the sphingolipid fraction (SLF), and sphingomyelin (SPM), plus mechanical stretching, on expression of the mRNAs of Dok-7, MuSK, and myogenin in differentiating C2C12 cells. Levels of expression of the mRNAs of Dok-7 (A), MuSK (B), and myogenin (C) were measured by using quantitative real-time PCR. Expression of each gene was normalized against that of the housekeeping gene encoding ribosomal protein, large, P0 (RPLP0/36B4). Values are means ± S.E. of 6 samples. *P < 0.05, significant difference vs. control group by Dunnett’s posthoc test. Values are expressed as ratios, using the value of the control group as 1.0.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125610&req=5

Fig5: Effects of milk-fat globule membrane (MFGM), the phospholipid fraction (PLF), the sphingolipid fraction (SLF), and sphingomyelin (SPM), plus mechanical stretching, on expression of the mRNAs of Dok-7, MuSK, and myogenin in differentiating C2C12 cells. Levels of expression of the mRNAs of Dok-7 (A), MuSK (B), and myogenin (C) were measured by using quantitative real-time PCR. Expression of each gene was normalized against that of the housekeeping gene encoding ribosomal protein, large, P0 (RPLP0/36B4). Values are means ± S.E. of 6 samples. *P < 0.05, significant difference vs. control group by Dunnett’s posthoc test. Values are expressed as ratios, using the value of the control group as 1.0.
Mentions: Skeletal muscle is highly adaptable and responds to exercise and training, thus increasing muscle mass and function. To further understand the mechanisms underlying the adaptive response of skeletal muscles in vivo, in vitro culture systems that use mechanical stretching of cultured myotubes have been developed to mimic in vivo muscle physiology (Passey et al. 2011), including neuromuscular adaptation in response to exercise (Folland and Williams 2007; Hubatsch and Jasmin 1997; Jasmin et al. 1991; Sveistrup et al. 1995). Accordingly, we examined the effects of MFGM-derived fractions and sphingomyelin combined with mechanical stretch (as a substitute for exercise in vivo) on the expression of several genes.Treatment of the stretched cells with MFGM, PLF, SLF, or sphingomyelin significantly increased Dok-7 gene expression compared with that in cells that received mechanical stretching alone; this was evident especially in the cells treated with SLF or sphingomyelin (Figure 5). Myogenin gene expression was increased significantly by treatment with 0.01% MFGM or with 0.001% or 0.005% PLF fraction plus mechanical stretching of the cells. These results are consistent with the effects of the combination of habitual exercise and dietary MFGM observed in vivo.Figure 5

Bottom Line: Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively).Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression.Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.

ABSTRACT
We examined the effects of habitual exercise plus nutritional intervention through consumption of milk fat globule membrane (MFGM), a milk component, on aging-related deficits in muscle mass and function in senescence-accelerated P1 mice. Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively). Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression. Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression. These findings suggest that habitual exercise plus dietary MFGM improves muscle function deficits through neuromuscular development, and that phospholipid and sphingolipid in MFGM contribute to its physiological actions.

No MeSH data available.