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Habitual exercise plus dietary supplementation with milk fat globule membrane improves muscle function deficits via neuromuscular development in senescence-accelerated mice.

Haramizu S, Mori T, Yano M, Ota N, Hashizume K, Otsuka A, Hase T, Shimotoyodome A - Springerplus (2014)

Bottom Line: Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively).Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression.Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.

ABSTRACT
We examined the effects of habitual exercise plus nutritional intervention through consumption of milk fat globule membrane (MFGM), a milk component, on aging-related deficits in muscle mass and function in senescence-accelerated P1 mice. Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively). Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression. Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression. These findings suggest that habitual exercise plus dietary MFGM improves muscle function deficits through neuromuscular development, and that phospholipid and sphingolipid in MFGM contribute to its physiological actions.

No MeSH data available.


Related in: MedlinePlus

Effects of milk-fat globule membrane (MFGM) on production of MuSK and Dok-7 proteins. Levels of production of the proteins MuSK (A), and Dok-7 (B) were measured by western blot analysis. The quadriceps muscle was removed from each mouse in the non-fasting, resting condition. Tissue lysates were then prepared and subjected to western blot analysis. Values are means ± S.E. of 7 or 8 mice. †P < 0.05, significant difference between ICR group and control SAMP1 group by unpaired t-test. **P < 0.01, significant difference vs. Control SAMP1 group by Fisher’s PLSD posthoc test. Values are expressed as ratios, using the value of the control SAMP1 group as 1.0. MFGMEx, MFGM plus habitual exercise.
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Fig4: Effects of milk-fat globule membrane (MFGM) on production of MuSK and Dok-7 proteins. Levels of production of the proteins MuSK (A), and Dok-7 (B) were measured by western blot analysis. The quadriceps muscle was removed from each mouse in the non-fasting, resting condition. Tissue lysates were then prepared and subjected to western blot analysis. Values are means ± S.E. of 7 or 8 mice. †P < 0.05, significant difference between ICR group and control SAMP1 group by unpaired t-test. **P < 0.01, significant difference vs. Control SAMP1 group by Fisher’s PLSD posthoc test. Values are expressed as ratios, using the value of the control SAMP1 group as 1.0. MFGMEx, MFGM plus habitual exercise.

Mentions: The Dok-7 protein level was significantly higher (+30.8%, P < 0.01), and the MuSK protein level tended to be higher (+18.2%, P = 0.1), in the MFGMEx group than in the control SAMP1 group (Figure 4). ICR mice had significantly more MuSK protein than the control SAMP1 mice. The Dok-7 protein level did not differ between these two groups.Figure 4


Habitual exercise plus dietary supplementation with milk fat globule membrane improves muscle function deficits via neuromuscular development in senescence-accelerated mice.

Haramizu S, Mori T, Yano M, Ota N, Hashizume K, Otsuka A, Hase T, Shimotoyodome A - Springerplus (2014)

Effects of milk-fat globule membrane (MFGM) on production of MuSK and Dok-7 proteins. Levels of production of the proteins MuSK (A), and Dok-7 (B) were measured by western blot analysis. The quadriceps muscle was removed from each mouse in the non-fasting, resting condition. Tissue lysates were then prepared and subjected to western blot analysis. Values are means ± S.E. of 7 or 8 mice. †P < 0.05, significant difference between ICR group and control SAMP1 group by unpaired t-test. **P < 0.01, significant difference vs. Control SAMP1 group by Fisher’s PLSD posthoc test. Values are expressed as ratios, using the value of the control SAMP1 group as 1.0. MFGMEx, MFGM plus habitual exercise.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125610&req=5

Fig4: Effects of milk-fat globule membrane (MFGM) on production of MuSK and Dok-7 proteins. Levels of production of the proteins MuSK (A), and Dok-7 (B) were measured by western blot analysis. The quadriceps muscle was removed from each mouse in the non-fasting, resting condition. Tissue lysates were then prepared and subjected to western blot analysis. Values are means ± S.E. of 7 or 8 mice. †P < 0.05, significant difference between ICR group and control SAMP1 group by unpaired t-test. **P < 0.01, significant difference vs. Control SAMP1 group by Fisher’s PLSD posthoc test. Values are expressed as ratios, using the value of the control SAMP1 group as 1.0. MFGMEx, MFGM plus habitual exercise.
Mentions: The Dok-7 protein level was significantly higher (+30.8%, P < 0.01), and the MuSK protein level tended to be higher (+18.2%, P = 0.1), in the MFGMEx group than in the control SAMP1 group (Figure 4). ICR mice had significantly more MuSK protein than the control SAMP1 mice. The Dok-7 protein level did not differ between these two groups.Figure 4

Bottom Line: Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively).Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression.Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.

ABSTRACT
We examined the effects of habitual exercise plus nutritional intervention through consumption of milk fat globule membrane (MFGM), a milk component, on aging-related deficits in muscle mass and function in senescence-accelerated P1 mice. Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively). Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression. Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression. These findings suggest that habitual exercise plus dietary MFGM improves muscle function deficits through neuromuscular development, and that phospholipid and sphingolipid in MFGM contribute to its physiological actions.

No MeSH data available.


Related in: MedlinePlus