Limits...
Habitual exercise plus dietary supplementation with milk fat globule membrane improves muscle function deficits via neuromuscular development in senescence-accelerated mice.

Haramizu S, Mori T, Yano M, Ota N, Hashizume K, Otsuka A, Hase T, Shimotoyodome A - Springerplus (2014)

Bottom Line: Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively).Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression.Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.

ABSTRACT
We examined the effects of habitual exercise plus nutritional intervention through consumption of milk fat globule membrane (MFGM), a milk component, on aging-related deficits in muscle mass and function in senescence-accelerated P1 mice. Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively). Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression. Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression. These findings suggest that habitual exercise plus dietary MFGM improves muscle function deficits through neuromuscular development, and that phospholipid and sphingolipid in MFGM contribute to its physiological actions.

No MeSH data available.


Related in: MedlinePlus

Effects of milk-fat globule membrane (MFGM) on expression of genes associated with nervous system development and function-related molecules. mRNA expression levels of MuSK (A), Dok-7 (B), NCAM (C), MyoD (D), and myogenin (E) were measured using quantitative real-time PCR. Expression of each gene was normalized against that of the housekeeping gene encoding ribosomal protein, large, P0 (RPLP0/36B4). Values are means ± S.E. of 7 or 8 mice. ††P < 0.01 and †P < 0.05, significant difference between ICR group and control SAMP1 group by unpaired t-test. *P < 0.05, significant difference vs. control SAMP1 group by Fisher’s PLSD posthoc test. Values are expressed as ratios, using the value of the control SAMP1 group as 1.0. MuSK, muscle skeletal receptor-tyrosine kinase; Dok-7, docking protein-7; NCAM, neural cell adhesion molecule; MyoD, myogenic differentiation; MFGMEx, MFGM plus habitual exercise.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4125610&req=5

Fig3: Effects of milk-fat globule membrane (MFGM) on expression of genes associated with nervous system development and function-related molecules. mRNA expression levels of MuSK (A), Dok-7 (B), NCAM (C), MyoD (D), and myogenin (E) were measured using quantitative real-time PCR. Expression of each gene was normalized against that of the housekeeping gene encoding ribosomal protein, large, P0 (RPLP0/36B4). Values are means ± S.E. of 7 or 8 mice. ††P < 0.01 and †P < 0.05, significant difference between ICR group and control SAMP1 group by unpaired t-test. *P < 0.05, significant difference vs. control SAMP1 group by Fisher’s PLSD posthoc test. Values are expressed as ratios, using the value of the control SAMP1 group as 1.0. MuSK, muscle skeletal receptor-tyrosine kinase; Dok-7, docking protein-7; NCAM, neural cell adhesion molecule; MyoD, myogenic differentiation; MFGMEx, MFGM plus habitual exercise.

Mentions: Expression of mRNAs for MyoD (+37.7%) and myogenin (+63.6%) was significantly higher in the MFGMEx group than in the control SAMP1 group (Figure 3), whereas it was significantly lower in the control SAMP1 group than in the ICR group. The MFGMEx group had significantly higher MuSK mRNA expression (+26.9%) than the control SAMP1 group, whereas MuSK mRNA expression did not differ between the ICR group and the control SAMP1 group. The MFGMEx group had significantly higher NCAM mRNA expression (+29.5%) than did the control SAMP1 group; these results were consistent with the microarray results. Whereas Dok-7 was not found in the microarray results (Additional file 1: Table S1), our RT-PCR analysis showed that the MFGMEx group had significantly greater Dok-7 (+31.0%) mRNA expression than the control SAMP1 group (Figure 3).Figure 3


Habitual exercise plus dietary supplementation with milk fat globule membrane improves muscle function deficits via neuromuscular development in senescence-accelerated mice.

Haramizu S, Mori T, Yano M, Ota N, Hashizume K, Otsuka A, Hase T, Shimotoyodome A - Springerplus (2014)

Effects of milk-fat globule membrane (MFGM) on expression of genes associated with nervous system development and function-related molecules. mRNA expression levels of MuSK (A), Dok-7 (B), NCAM (C), MyoD (D), and myogenin (E) were measured using quantitative real-time PCR. Expression of each gene was normalized against that of the housekeeping gene encoding ribosomal protein, large, P0 (RPLP0/36B4). Values are means ± S.E. of 7 or 8 mice. ††P < 0.01 and †P < 0.05, significant difference between ICR group and control SAMP1 group by unpaired t-test. *P < 0.05, significant difference vs. control SAMP1 group by Fisher’s PLSD posthoc test. Values are expressed as ratios, using the value of the control SAMP1 group as 1.0. MuSK, muscle skeletal receptor-tyrosine kinase; Dok-7, docking protein-7; NCAM, neural cell adhesion molecule; MyoD, myogenic differentiation; MFGMEx, MFGM plus habitual exercise.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125610&req=5

Fig3: Effects of milk-fat globule membrane (MFGM) on expression of genes associated with nervous system development and function-related molecules. mRNA expression levels of MuSK (A), Dok-7 (B), NCAM (C), MyoD (D), and myogenin (E) were measured using quantitative real-time PCR. Expression of each gene was normalized against that of the housekeeping gene encoding ribosomal protein, large, P0 (RPLP0/36B4). Values are means ± S.E. of 7 or 8 mice. ††P < 0.01 and †P < 0.05, significant difference between ICR group and control SAMP1 group by unpaired t-test. *P < 0.05, significant difference vs. control SAMP1 group by Fisher’s PLSD posthoc test. Values are expressed as ratios, using the value of the control SAMP1 group as 1.0. MuSK, muscle skeletal receptor-tyrosine kinase; Dok-7, docking protein-7; NCAM, neural cell adhesion molecule; MyoD, myogenic differentiation; MFGMEx, MFGM plus habitual exercise.
Mentions: Expression of mRNAs for MyoD (+37.7%) and myogenin (+63.6%) was significantly higher in the MFGMEx group than in the control SAMP1 group (Figure 3), whereas it was significantly lower in the control SAMP1 group than in the ICR group. The MFGMEx group had significantly higher MuSK mRNA expression (+26.9%) than the control SAMP1 group, whereas MuSK mRNA expression did not differ between the ICR group and the control SAMP1 group. The MFGMEx group had significantly higher NCAM mRNA expression (+29.5%) than did the control SAMP1 group; these results were consistent with the microarray results. Whereas Dok-7 was not found in the microarray results (Additional file 1: Table S1), our RT-PCR analysis showed that the MFGMEx group had significantly greater Dok-7 (+31.0%) mRNA expression than the control SAMP1 group (Figure 3).Figure 3

Bottom Line: Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively).Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression.Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.

ABSTRACT
We examined the effects of habitual exercise plus nutritional intervention through consumption of milk fat globule membrane (MFGM), a milk component, on aging-related deficits in muscle mass and function in senescence-accelerated P1 mice. Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively). Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression. Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression. These findings suggest that habitual exercise plus dietary MFGM improves muscle function deficits through neuromuscular development, and that phospholipid and sphingolipid in MFGM contribute to its physiological actions.

No MeSH data available.


Related in: MedlinePlus