Limits...
Habitual exercise plus dietary supplementation with milk fat globule membrane improves muscle function deficits via neuromuscular development in senescence-accelerated mice.

Haramizu S, Mori T, Yano M, Ota N, Hashizume K, Otsuka A, Hase T, Shimotoyodome A - Springerplus (2014)

Bottom Line: Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively).Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression.Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.

ABSTRACT
We examined the effects of habitual exercise plus nutritional intervention through consumption of milk fat globule membrane (MFGM), a milk component, on aging-related deficits in muscle mass and function in senescence-accelerated P1 mice. Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively). Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression. Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression. These findings suggest that habitual exercise plus dietary MFGM improves muscle function deficits through neuromuscular development, and that phospholipid and sphingolipid in MFGM contribute to its physiological actions.

No MeSH data available.


Effects of milk-fat globule membrane (MFGM) on expression of genes associated with insulin-like growth factor (IGF)-1 signaling-related molecules. mRNA expression levels of IGF-1 (A), IGF-1 receptor (B), and IGF-binding protein (IGFBP)-5 (C) were measured using quantitative real-time PCR. Values are means ± S.E. of 7 or 8 mice. ††P < 0.01, significant difference between ICR group and control SAMP1 group by unpaired t-test. *P < 0.05, significant difference vs. control SAMP1 group by Fisher’s PLSD posthoc test. Values are expressed as ratios, using the value of the control SAMP1 group as 1.0. MFGMEx, MFGM plus habitual exercise.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4125610&req=5

Fig2: Effects of milk-fat globule membrane (MFGM) on expression of genes associated with insulin-like growth factor (IGF)-1 signaling-related molecules. mRNA expression levels of IGF-1 (A), IGF-1 receptor (B), and IGF-binding protein (IGFBP)-5 (C) were measured using quantitative real-time PCR. Values are means ± S.E. of 7 or 8 mice. ††P < 0.01, significant difference between ICR group and control SAMP1 group by unpaired t-test. *P < 0.05, significant difference vs. control SAMP1 group by Fisher’s PLSD posthoc test. Values are expressed as ratios, using the value of the control SAMP1 group as 1.0. MFGMEx, MFGM plus habitual exercise.

Mentions: To explore the differences in muscle weight between the SAMP1 controls and the ICR controls and (in the case of the quadriceps) between the MFGMEx group and the SAMP1 controls, the genes involved in muscle development were assessed by using RT-PCR. The control SAMP1 group had significantly lower igf1r and higher IGF-1 binding protein (igfbp)-5 gene expression (18.8% and 32.2%, respectively) than the ICR group. IGF-1 mRNA expression level did not differ among the groups (Figure 2). The MFGMEx group had significantly higher igf1r and lower igfbp5 gene expression (by 23.4% and 24.3%, respectively) than did the control SAMP1 group.Figure 2


Habitual exercise plus dietary supplementation with milk fat globule membrane improves muscle function deficits via neuromuscular development in senescence-accelerated mice.

Haramizu S, Mori T, Yano M, Ota N, Hashizume K, Otsuka A, Hase T, Shimotoyodome A - Springerplus (2014)

Effects of milk-fat globule membrane (MFGM) on expression of genes associated with insulin-like growth factor (IGF)-1 signaling-related molecules. mRNA expression levels of IGF-1 (A), IGF-1 receptor (B), and IGF-binding protein (IGFBP)-5 (C) were measured using quantitative real-time PCR. Values are means ± S.E. of 7 or 8 mice. ††P < 0.01, significant difference between ICR group and control SAMP1 group by unpaired t-test. *P < 0.05, significant difference vs. control SAMP1 group by Fisher’s PLSD posthoc test. Values are expressed as ratios, using the value of the control SAMP1 group as 1.0. MFGMEx, MFGM plus habitual exercise.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125610&req=5

Fig2: Effects of milk-fat globule membrane (MFGM) on expression of genes associated with insulin-like growth factor (IGF)-1 signaling-related molecules. mRNA expression levels of IGF-1 (A), IGF-1 receptor (B), and IGF-binding protein (IGFBP)-5 (C) were measured using quantitative real-time PCR. Values are means ± S.E. of 7 or 8 mice. ††P < 0.01, significant difference between ICR group and control SAMP1 group by unpaired t-test. *P < 0.05, significant difference vs. control SAMP1 group by Fisher’s PLSD posthoc test. Values are expressed as ratios, using the value of the control SAMP1 group as 1.0. MFGMEx, MFGM plus habitual exercise.
Mentions: To explore the differences in muscle weight between the SAMP1 controls and the ICR controls and (in the case of the quadriceps) between the MFGMEx group and the SAMP1 controls, the genes involved in muscle development were assessed by using RT-PCR. The control SAMP1 group had significantly lower igf1r and higher IGF-1 binding protein (igfbp)-5 gene expression (18.8% and 32.2%, respectively) than the ICR group. IGF-1 mRNA expression level did not differ among the groups (Figure 2). The MFGMEx group had significantly higher igf1r and lower igfbp5 gene expression (by 23.4% and 24.3%, respectively) than did the control SAMP1 group.Figure 2

Bottom Line: Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively).Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression.Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.

ABSTRACT
We examined the effects of habitual exercise plus nutritional intervention through consumption of milk fat globule membrane (MFGM), a milk component, on aging-related deficits in muscle mass and function in senescence-accelerated P1 mice. Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively). Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression. Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression. These findings suggest that habitual exercise plus dietary MFGM improves muscle function deficits through neuromuscular development, and that phospholipid and sphingolipid in MFGM contribute to its physiological actions.

No MeSH data available.