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Habitual exercise plus dietary supplementation with milk fat globule membrane improves muscle function deficits via neuromuscular development in senescence-accelerated mice.

Haramizu S, Mori T, Yano M, Ota N, Hashizume K, Otsuka A, Hase T, Shimotoyodome A - Springerplus (2014)

Bottom Line: Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively).Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression.Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.

ABSTRACT
We examined the effects of habitual exercise plus nutritional intervention through consumption of milk fat globule membrane (MFGM), a milk component, on aging-related deficits in muscle mass and function in senescence-accelerated P1 mice. Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively). Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression. Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression. These findings suggest that habitual exercise plus dietary MFGM improves muscle function deficits through neuromuscular development, and that phospholipid and sphingolipid in MFGM contribute to its physiological actions.

No MeSH data available.


Effects of milk-fat globule membrane (MFGM) or green tea extract (GTE) on tetanic contractile force of soleus and extensor digitorum longus (EDL) muscles. The tetanic contractile force of the isolated soleus (A) and EDL (B) muscles was measured as described in the Methods. Values are means ± S.E. of 7 or 8 mice. ††P < 0.01 and ‡‡P < 0.0001, significant difference between ICR group and control SAMP1 group by unpaired t-test. *P < 0.05, significant difference vs. the control SAMP1 group by Dunnett’s posthoc test. MFGMEx, MFGM plus habitual exercise; GTEEx, GTE plus habitual exercise.
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Fig1: Effects of milk-fat globule membrane (MFGM) or green tea extract (GTE) on tetanic contractile force of soleus and extensor digitorum longus (EDL) muscles. The tetanic contractile force of the isolated soleus (A) and EDL (B) muscles was measured as described in the Methods. Values are means ± S.E. of 7 or 8 mice. ††P < 0.01 and ‡‡P < 0.0001, significant difference between ICR group and control SAMP1 group by unpaired t-test. *P < 0.05, significant difference vs. the control SAMP1 group by Dunnett’s posthoc test. MFGMEx, MFGM plus habitual exercise; GTEEx, GTE plus habitual exercise.

Mentions: The tetanic contractile force of the soleus (Figure 1A) and EDL (Figure 1B) muscles was significantly lower in the control SAMP1 group than in the ICR group at the age of 43 wk. The MFGMEx group had significantly higher tetanic contractile force than the control SAMP1 group in the soleus (Figure 1A) and EDL (Figure 1B) muscles. Contractile force in the GTEEx group did not differ significantly from that in the control SAMP1 group.Figure 1


Habitual exercise plus dietary supplementation with milk fat globule membrane improves muscle function deficits via neuromuscular development in senescence-accelerated mice.

Haramizu S, Mori T, Yano M, Ota N, Hashizume K, Otsuka A, Hase T, Shimotoyodome A - Springerplus (2014)

Effects of milk-fat globule membrane (MFGM) or green tea extract (GTE) on tetanic contractile force of soleus and extensor digitorum longus (EDL) muscles. The tetanic contractile force of the isolated soleus (A) and EDL (B) muscles was measured as described in the Methods. Values are means ± S.E. of 7 or 8 mice. ††P < 0.01 and ‡‡P < 0.0001, significant difference between ICR group and control SAMP1 group by unpaired t-test. *P < 0.05, significant difference vs. the control SAMP1 group by Dunnett’s posthoc test. MFGMEx, MFGM plus habitual exercise; GTEEx, GTE plus habitual exercise.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125610&req=5

Fig1: Effects of milk-fat globule membrane (MFGM) or green tea extract (GTE) on tetanic contractile force of soleus and extensor digitorum longus (EDL) muscles. The tetanic contractile force of the isolated soleus (A) and EDL (B) muscles was measured as described in the Methods. Values are means ± S.E. of 7 or 8 mice. ††P < 0.01 and ‡‡P < 0.0001, significant difference between ICR group and control SAMP1 group by unpaired t-test. *P < 0.05, significant difference vs. the control SAMP1 group by Dunnett’s posthoc test. MFGMEx, MFGM plus habitual exercise; GTEEx, GTE plus habitual exercise.
Mentions: The tetanic contractile force of the soleus (Figure 1A) and EDL (Figure 1B) muscles was significantly lower in the control SAMP1 group than in the ICR group at the age of 43 wk. The MFGMEx group had significantly higher tetanic contractile force than the control SAMP1 group in the soleus (Figure 1A) and EDL (Figure 1B) muscles. Contractile force in the GTEEx group did not differ significantly from that in the control SAMP1 group.Figure 1

Bottom Line: Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively).Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression.Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: Biological Science Laboratories, Kao Corporation, Tochigi, Japan.

ABSTRACT
We examined the effects of habitual exercise plus nutritional intervention through consumption of milk fat globule membrane (MFGM), a milk component, on aging-related deficits in muscle mass and function in senescence-accelerated P1 mice. Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively). Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression. Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression. These findings suggest that habitual exercise plus dietary MFGM improves muscle function deficits through neuromuscular development, and that phospholipid and sphingolipid in MFGM contribute to its physiological actions.

No MeSH data available.