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Effects of L-carnitine supplementation on oxidative stress and antioxidant enzymes activities in patients with coronary artery disease: a randomized, placebo-controlled trial.

Lee BJ, Lin JS, Lin YC, Lin PT - Nutr J (2014)

Bottom Line: Thirty-nine subjects completed the study (placebo, n = 19; LC, n = 20).LC supplementation at a dose of 1000 mg/d was associated with a significant reduction in oxidative stress and an increase in antioxidant enzymes activities in CAD patients.CAD patients might benefit from using LC supplements to increase their anti-oxidation capacity.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Nutrition, Chung Shan Medical University, Taichung 40201, Taiwan. apt810@csmu.edu.tw.

ABSTRACT

Background: Cardiovascular disease is the leading cause of death worldwide. Higher oxidative stress may contribute to the pathogenesis of coronary artery disease (CAD). The purpose of this study was to investigate the effect of L-carnitine (LC, 1000 mg/d) on the markers of oxidative stress and antioxidant enzymes activities in CAD patients.

Methods: We enrolled 47 CAD patients in the study. The CAD patients were identified by cardiac catheterization as having at least 50% stenosis of one major coronary artery. The subjects were randomly assigned to the placebo (n = 24) and LC (n = 23) groups. The intervention was administered for 12 weeks. The levels of serum LC, plasma malondialdehyde (MDA), and erythrocyte antioxidant enzymes activities [catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx)] were measured before and after intervention.

Results: Thirty-nine subjects completed the study (placebo, n = 19; LC, n = 20). After 12 weeks of LC supplementation, the level of MDA was significantly reduced (2.0 ± 0.3 to 1.8 ± 0.3 μmol/L, P = 0.02) and the level of LC (33.6 ± 13.6 to 40.0 ± 12.0 μmol/L, P = 0.04) and antioxidant enzymes activities [CAT (12.7 ± 5.5 to 13.1 ± 5.8 U/mg of protein, P = 0.02), SOD (14.8 ± 2.9 to 20.7 ± 5.8 U/mg of protein, P < 0.01), and GPx (20.3 ± 3.4 to 23.0 ± 3.1 U/mg of protein, P = 0.01)] were significantly increased. The level of LC was significantly positively correlated with the antioxidant enzymes activities (CAT, β = 0.87, P = 0.02; SOD, β = 0.72, P < 0.01).

Conclusion: LC supplementation at a dose of 1000 mg/d was associated with a significant reduction in oxidative stress and an increase in antioxidant enzymes activities in CAD patients. CAD patients might benefit from using LC supplements to increase their anti-oxidation capacity.

Trial registration: Clinical Trials.gov Identifier: NCT01819701.

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Related in: MedlinePlus

Levels of L-carnitine, oxidative stress, and antioxidant enzymes activities after supplementation. Data are means ± SD. □ week 0, ■ week 12. *Values were significantly different after intervention within the group. a, bValues with different superscripts were significantly different between the two groups. CAT, catalase; GPx, glutathione peroxidase; LC, L-carnitine; MDA, malondialdehyde; SOD, superoxide dismutase.
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Figure 2: Levels of L-carnitine, oxidative stress, and antioxidant enzymes activities after supplementation. Data are means ± SD. □ week 0, ■ week 12. *Values were significantly different after intervention within the group. a, bValues with different superscripts were significantly different between the two groups. CAT, catalase; GPx, glutathione peroxidase; LC, L-carnitine; MDA, malondialdehyde; SOD, superoxide dismutase.

Mentions: The levels of LC, oxidative stress, and antioxidant enzymes activities are shown in Figure 2. The subjects in the LC group had significantly lower level of MDA (1.8 ± 0.3 versus 2.0 ± 0.4 μmol/L, P = 0.01), higher levels of LC (40.0 ± 12.0 versus 35.2 ± 12.0 μmol/L, P = 0.02), and higher activities of CAT (13.1 ± 5.8 versus 10.6 ± 2.9 U/mg of protein, P < 0.01), SOD (20.7 ± 4.2 versus 13.1 ± 2.9 U/mg of protein, P < 0.01), and GPx (23.0 ± 3.1 versus 19.1 ± 2.3 U/mg of protein, P < 0.01) than those in the placebo group at week 12. After LC supplementation, the level of MDA was significantly reduced (2.0 ± 0.3 to 1.8 ± 0.3 μmol/L, P = 0.02) and levels of LC (33.6 ± 13.6 to 40.0 ± 12.0 μmol/L, P = 0.04) and antioxidant enzymes activities (CAT, 12.7 ± 5.5 to 13.1 ± 5.8 U/ mg of protein, P = 0.02; SOD, 14.8 ± 2.9 to 20.7 ± 4.2 U/ mg of protein, P < 0.01; and GPx, 20.3 ± 3.4 to 23.0 ± 3.1 U/mg of protein, P = 0.01) were significantly increased from the baseline.


Effects of L-carnitine supplementation on oxidative stress and antioxidant enzymes activities in patients with coronary artery disease: a randomized, placebo-controlled trial.

Lee BJ, Lin JS, Lin YC, Lin PT - Nutr J (2014)

Levels of L-carnitine, oxidative stress, and antioxidant enzymes activities after supplementation. Data are means ± SD. □ week 0, ■ week 12. *Values were significantly different after intervention within the group. a, bValues with different superscripts were significantly different between the two groups. CAT, catalase; GPx, glutathione peroxidase; LC, L-carnitine; MDA, malondialdehyde; SOD, superoxide dismutase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4125592&req=5

Figure 2: Levels of L-carnitine, oxidative stress, and antioxidant enzymes activities after supplementation. Data are means ± SD. □ week 0, ■ week 12. *Values were significantly different after intervention within the group. a, bValues with different superscripts were significantly different between the two groups. CAT, catalase; GPx, glutathione peroxidase; LC, L-carnitine; MDA, malondialdehyde; SOD, superoxide dismutase.
Mentions: The levels of LC, oxidative stress, and antioxidant enzymes activities are shown in Figure 2. The subjects in the LC group had significantly lower level of MDA (1.8 ± 0.3 versus 2.0 ± 0.4 μmol/L, P = 0.01), higher levels of LC (40.0 ± 12.0 versus 35.2 ± 12.0 μmol/L, P = 0.02), and higher activities of CAT (13.1 ± 5.8 versus 10.6 ± 2.9 U/mg of protein, P < 0.01), SOD (20.7 ± 4.2 versus 13.1 ± 2.9 U/mg of protein, P < 0.01), and GPx (23.0 ± 3.1 versus 19.1 ± 2.3 U/mg of protein, P < 0.01) than those in the placebo group at week 12. After LC supplementation, the level of MDA was significantly reduced (2.0 ± 0.3 to 1.8 ± 0.3 μmol/L, P = 0.02) and levels of LC (33.6 ± 13.6 to 40.0 ± 12.0 μmol/L, P = 0.04) and antioxidant enzymes activities (CAT, 12.7 ± 5.5 to 13.1 ± 5.8 U/ mg of protein, P = 0.02; SOD, 14.8 ± 2.9 to 20.7 ± 4.2 U/ mg of protein, P < 0.01; and GPx, 20.3 ± 3.4 to 23.0 ± 3.1 U/mg of protein, P = 0.01) were significantly increased from the baseline.

Bottom Line: Thirty-nine subjects completed the study (placebo, n = 19; LC, n = 20).LC supplementation at a dose of 1000 mg/d was associated with a significant reduction in oxidative stress and an increase in antioxidant enzymes activities in CAD patients.CAD patients might benefit from using LC supplements to increase their anti-oxidation capacity.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Nutrition, Chung Shan Medical University, Taichung 40201, Taiwan. apt810@csmu.edu.tw.

ABSTRACT

Background: Cardiovascular disease is the leading cause of death worldwide. Higher oxidative stress may contribute to the pathogenesis of coronary artery disease (CAD). The purpose of this study was to investigate the effect of L-carnitine (LC, 1000 mg/d) on the markers of oxidative stress and antioxidant enzymes activities in CAD patients.

Methods: We enrolled 47 CAD patients in the study. The CAD patients were identified by cardiac catheterization as having at least 50% stenosis of one major coronary artery. The subjects were randomly assigned to the placebo (n = 24) and LC (n = 23) groups. The intervention was administered for 12 weeks. The levels of serum LC, plasma malondialdehyde (MDA), and erythrocyte antioxidant enzymes activities [catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx)] were measured before and after intervention.

Results: Thirty-nine subjects completed the study (placebo, n = 19; LC, n = 20). After 12 weeks of LC supplementation, the level of MDA was significantly reduced (2.0 ± 0.3 to 1.8 ± 0.3 μmol/L, P = 0.02) and the level of LC (33.6 ± 13.6 to 40.0 ± 12.0 μmol/L, P = 0.04) and antioxidant enzymes activities [CAT (12.7 ± 5.5 to 13.1 ± 5.8 U/mg of protein, P = 0.02), SOD (14.8 ± 2.9 to 20.7 ± 5.8 U/mg of protein, P < 0.01), and GPx (20.3 ± 3.4 to 23.0 ± 3.1 U/mg of protein, P = 0.01)] were significantly increased. The level of LC was significantly positively correlated with the antioxidant enzymes activities (CAT, β = 0.87, P = 0.02; SOD, β = 0.72, P < 0.01).

Conclusion: LC supplementation at a dose of 1000 mg/d was associated with a significant reduction in oxidative stress and an increase in antioxidant enzymes activities in CAD patients. CAD patients might benefit from using LC supplements to increase their anti-oxidation capacity.

Trial registration: Clinical Trials.gov Identifier: NCT01819701.

Show MeSH
Related in: MedlinePlus