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The effects of bone morphogenetic protein-2 and enamel matrix derivative on the bioactivity of mineral trioxide aggregate in MC3T3-E1cells.

Jeong Y, Yang W, Ko H, Kim M - Restor Dent Endod (2014)

Bottom Line: ALP expression largely decreased in the EMD and MTA/EMD groups based on ALP staining.In the MTA/BMP-2 group, mRNA expression of OPN on day 3 and BSP and OCN on day 7 significantly increased.In the MTA/EMD group, OSN and OCN gene expression significantly increased on day 7, whereas ALP expression decreased on days 3 and 7 (p < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Conservative Dentistry, Ulsan University Asan Medical Center, Seoul, Korea.

ABSTRACT

Objectives: The effects of bone morphogenetic protein-2 (BMP-2) and enamel matrix derivative (EMD) respectively with mineral trioxide aggregate (MTA) on hard tissue regeneration have been investigated in previous studies. This study aimed to compare the osteogenic effects of MTA/BMP-2 and MTA/EMD treatment in MC3T3-E1 cells.

Materials and methods: MC3T3-E1 cells were treated with MTA (ProRoot, Dentsply), BMP-2 (R&D Systems), EMD (Emdogain, Straumann) separately and MTA/BMP-2 or MTA/EMD combination. Mineralization was evaluated by staining the calcium deposits with alkaline phosphatase (ALP, Sigma-Aldrich) and Alizarin red (Sigma-Aldrich). The effects on the osteoblast differentiation were evaluated by the expressions of osteogenic markers, including ALP, bone sialoprotein (BSP), osteocalcin (OCN), osteopontin (OPN) and osteonectin (OSN), as determined by reverse-transcription polymerase chain reaction analysis (RT-PCR, AccuPower PCR, Bioneer).

Results: Mineralization increased in the BMP-2 and MTA/BMP-2 groups and increased to a lesser extent in the MTA/EMD group but appeared to decrease in the MTA-only group based on Alizarin red staining. ALP expression largely decreased in the EMD and MTA/EMD groups based on ALP staining. In the MTA/BMP-2 group, mRNA expression of OPN on day 3 and BSP and OCN on day 7 significantly increased. In the MTA/EMD group, OSN and OCN gene expression significantly increased on day 7, whereas ALP expression decreased on days 3 and 7 (p < 0.05).

Conclusions: These results suggest the MTA/BMP-2 combination promoted more rapid differentiation in MC3T3-E1 cells than did MTA/EMD during the early mineralization period.

No MeSH data available.


Related in: MedlinePlus

Formation of calcification nodules in each group in MC3T3-E1 cells. MC3T3-E1 cells were cultured with or without treated materials for 7 days and stained with Alizarin red. A representative photograph of Alizarin red staining is shown, (a) control; (b) EMD; (c) BMP-2; (d) MTA; (e) MTA/EMD; (f) MTA/BMP-2. EMD, enamel matrix derivative; BMP, bone morphogenetic protein; MTA, mineral trioxide aggregate.
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Figure 2: Formation of calcification nodules in each group in MC3T3-E1 cells. MC3T3-E1 cells were cultured with or without treated materials for 7 days and stained with Alizarin red. A representative photograph of Alizarin red staining is shown, (a) control; (b) EMD; (c) BMP-2; (d) MTA; (e) MTA/EMD; (f) MTA/BMP-2. EMD, enamel matrix derivative; BMP, bone morphogenetic protein; MTA, mineral trioxide aggregate.

Mentions: Alizarin red staining for calcium revealed largely increased mineralization in the BMP-2- and MTA/BMP-2-treated groups and increased to a lesser extent in the EMD and MTA/EMD groups. However, the amount of mineralization showed a trend toward decreasing in the MTA-only cells compared to the negative control cells (Figure 2).


The effects of bone morphogenetic protein-2 and enamel matrix derivative on the bioactivity of mineral trioxide aggregate in MC3T3-E1cells.

Jeong Y, Yang W, Ko H, Kim M - Restor Dent Endod (2014)

Formation of calcification nodules in each group in MC3T3-E1 cells. MC3T3-E1 cells were cultured with or without treated materials for 7 days and stained with Alizarin red. A representative photograph of Alizarin red staining is shown, (a) control; (b) EMD; (c) BMP-2; (d) MTA; (e) MTA/EMD; (f) MTA/BMP-2. EMD, enamel matrix derivative; BMP, bone morphogenetic protein; MTA, mineral trioxide aggregate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125582&req=5

Figure 2: Formation of calcification nodules in each group in MC3T3-E1 cells. MC3T3-E1 cells were cultured with or without treated materials for 7 days and stained with Alizarin red. A representative photograph of Alizarin red staining is shown, (a) control; (b) EMD; (c) BMP-2; (d) MTA; (e) MTA/EMD; (f) MTA/BMP-2. EMD, enamel matrix derivative; BMP, bone morphogenetic protein; MTA, mineral trioxide aggregate.
Mentions: Alizarin red staining for calcium revealed largely increased mineralization in the BMP-2- and MTA/BMP-2-treated groups and increased to a lesser extent in the EMD and MTA/EMD groups. However, the amount of mineralization showed a trend toward decreasing in the MTA-only cells compared to the negative control cells (Figure 2).

Bottom Line: ALP expression largely decreased in the EMD and MTA/EMD groups based on ALP staining.In the MTA/BMP-2 group, mRNA expression of OPN on day 3 and BSP and OCN on day 7 significantly increased.In the MTA/EMD group, OSN and OCN gene expression significantly increased on day 7, whereas ALP expression decreased on days 3 and 7 (p < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Conservative Dentistry, Ulsan University Asan Medical Center, Seoul, Korea.

ABSTRACT

Objectives: The effects of bone morphogenetic protein-2 (BMP-2) and enamel matrix derivative (EMD) respectively with mineral trioxide aggregate (MTA) on hard tissue regeneration have been investigated in previous studies. This study aimed to compare the osteogenic effects of MTA/BMP-2 and MTA/EMD treatment in MC3T3-E1 cells.

Materials and methods: MC3T3-E1 cells were treated with MTA (ProRoot, Dentsply), BMP-2 (R&D Systems), EMD (Emdogain, Straumann) separately and MTA/BMP-2 or MTA/EMD combination. Mineralization was evaluated by staining the calcium deposits with alkaline phosphatase (ALP, Sigma-Aldrich) and Alizarin red (Sigma-Aldrich). The effects on the osteoblast differentiation were evaluated by the expressions of osteogenic markers, including ALP, bone sialoprotein (BSP), osteocalcin (OCN), osteopontin (OPN) and osteonectin (OSN), as determined by reverse-transcription polymerase chain reaction analysis (RT-PCR, AccuPower PCR, Bioneer).

Results: Mineralization increased in the BMP-2 and MTA/BMP-2 groups and increased to a lesser extent in the MTA/EMD group but appeared to decrease in the MTA-only group based on Alizarin red staining. ALP expression largely decreased in the EMD and MTA/EMD groups based on ALP staining. In the MTA/BMP-2 group, mRNA expression of OPN on day 3 and BSP and OCN on day 7 significantly increased. In the MTA/EMD group, OSN and OCN gene expression significantly increased on day 7, whereas ALP expression decreased on days 3 and 7 (p < 0.05).

Conclusions: These results suggest the MTA/BMP-2 combination promoted more rapid differentiation in MC3T3-E1 cells than did MTA/EMD during the early mineralization period.

No MeSH data available.


Related in: MedlinePlus