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Enamel pretreatment with Er:YAG laser: effects on the microleakage of fissure sealant in fluorosed teeth.

Memarpour M, Kianimanesh N, Shayeghi B - Restor Dent Endod (2014)

Bottom Line: Overall, there were significant differences among all groups in the PM (p = 0.00).Group 3 showed the greatest PM, and was significantly different from groups 2 to 6 (p < 0.05).There were no significant differences among groups in PUA.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Objectives: The purpose of this in vitro study was to evaluate the microleakage and penetration of fissure sealant in permanent molar teeth with fluorosis after pretreatment of the occlusal surface.

Materials and methods: A total of 120 third molars with mild dental fluorosis were randomly divided into 6 groups (n = 20). The tooth surfaces were sealed with an unfilled resin fissure sealant (FS) material. The experimental groups included: 1) phosphoric acid etching (AE) + FS (control); 2) AE + One-Step Plus (OS, Bisco) + FS; 3) bur + AE + FS; 4) bur + AE + OS + FS; 5) Er:YAG laser + AE + FS; and 6) Er:YAG laser + AE + OS + FS. After thermocycling, the teeth were immersed in 0.5% fuchsin and sectioned. Proportions of mircoleakage (PM) and unfilled area (PUA) were measured by digital microscope.

Results: Overall, there were significant differences among all groups in the PM (p = 0.00). Group 3 showed the greatest PM, and was significantly different from groups 2 to 6 (p < 0.05). Group 6 showed the lowest PM. Pretreatment with Er:YAG with or without adhesive led to less PM than bur pretreatment. There were no significant differences among groups in PUA.

Conclusions: Conventional acid etching provided a similar degree of occlusal seal in teeth with fluorosis compared to those pretreated with a bur or Er:YAG laser. Pretreatment of pits and fissures with Er:YAG in teeth with fluorosis may be an alternative method before fissure sealant application.

No MeSH data available.


Related in: MedlinePlus

Method used to calculate the proportion of the unfilled area: a / (a + b). b represents the fissure sealant area and a represents the unfilled area.21,28
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Figure 2: Method used to calculate the proportion of the unfilled area: a / (a + b). b represents the fissure sealant area and a represents the unfilled area.21,28

Mentions: All teeth underwent thermal cycling for 1,000 cycles between 5℃ and 55℃ in a water bath, with a dwell time of 30 seconds and a 20-seconds transit time between the baths. The apices were sealed with sticky wax, and all tooth surfaces were covered with 2 layers of nail polish except for a 1-mm zone around the margins of each FS. To test for microleakage the samples were immersed in 0.5% basic fuchsin (Merck, Darmstadt, Germany) dye solution for 24 hours. The specimens were rinsed to remove excess dye and each tooth was sectioned bucolingually across the center of the sealant with a diamond saw (Letiz 1600, Leica, Wetzlar, Germany) and continuous water irrigation. Under blind conditions, two observers measured linear dye penetration in millimeters from the margin of the FS along the tooth and the sealant under a digital microscope (Dino Lite, Taipei, Taiwan) at ×50 magnification. The microscope was calibrated before evaluations. Consistency between examiners was ensured by measuring microleakage in 10 sectioned teeth. Proportion of microleakage (PM) was calculated by dividing the total length of dye penetration (c + d) by the total length of the enamel sealant interface (e + f, Figure 1). Penetration of the sealant was recorded as the unfilled area (mm2) divided by the total FS area (mm2) to obtain the proportion of unfilled area (PUA, a / (a + b), Figure 2).21,28 After the data were recorded, statistical analyses were done with the Kruskal-Wallis test to compare all groups together and the Mann-Whitney test for pairwise comparisons (p < 0.05).


Enamel pretreatment with Er:YAG laser: effects on the microleakage of fissure sealant in fluorosed teeth.

Memarpour M, Kianimanesh N, Shayeghi B - Restor Dent Endod (2014)

Method used to calculate the proportion of the unfilled area: a / (a + b). b represents the fissure sealant area and a represents the unfilled area.21,28
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125581&req=5

Figure 2: Method used to calculate the proportion of the unfilled area: a / (a + b). b represents the fissure sealant area and a represents the unfilled area.21,28
Mentions: All teeth underwent thermal cycling for 1,000 cycles between 5℃ and 55℃ in a water bath, with a dwell time of 30 seconds and a 20-seconds transit time between the baths. The apices were sealed with sticky wax, and all tooth surfaces were covered with 2 layers of nail polish except for a 1-mm zone around the margins of each FS. To test for microleakage the samples were immersed in 0.5% basic fuchsin (Merck, Darmstadt, Germany) dye solution for 24 hours. The specimens were rinsed to remove excess dye and each tooth was sectioned bucolingually across the center of the sealant with a diamond saw (Letiz 1600, Leica, Wetzlar, Germany) and continuous water irrigation. Under blind conditions, two observers measured linear dye penetration in millimeters from the margin of the FS along the tooth and the sealant under a digital microscope (Dino Lite, Taipei, Taiwan) at ×50 magnification. The microscope was calibrated before evaluations. Consistency between examiners was ensured by measuring microleakage in 10 sectioned teeth. Proportion of microleakage (PM) was calculated by dividing the total length of dye penetration (c + d) by the total length of the enamel sealant interface (e + f, Figure 1). Penetration of the sealant was recorded as the unfilled area (mm2) divided by the total FS area (mm2) to obtain the proportion of unfilled area (PUA, a / (a + b), Figure 2).21,28 After the data were recorded, statistical analyses were done with the Kruskal-Wallis test to compare all groups together and the Mann-Whitney test for pairwise comparisons (p < 0.05).

Bottom Line: Overall, there were significant differences among all groups in the PM (p = 0.00).Group 3 showed the greatest PM, and was significantly different from groups 2 to 6 (p < 0.05).There were no significant differences among groups in PUA.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Objectives: The purpose of this in vitro study was to evaluate the microleakage and penetration of fissure sealant in permanent molar teeth with fluorosis after pretreatment of the occlusal surface.

Materials and methods: A total of 120 third molars with mild dental fluorosis were randomly divided into 6 groups (n = 20). The tooth surfaces were sealed with an unfilled resin fissure sealant (FS) material. The experimental groups included: 1) phosphoric acid etching (AE) + FS (control); 2) AE + One-Step Plus (OS, Bisco) + FS; 3) bur + AE + FS; 4) bur + AE + OS + FS; 5) Er:YAG laser + AE + FS; and 6) Er:YAG laser + AE + OS + FS. After thermocycling, the teeth were immersed in 0.5% fuchsin and sectioned. Proportions of mircoleakage (PM) and unfilled area (PUA) were measured by digital microscope.

Results: Overall, there were significant differences among all groups in the PM (p = 0.00). Group 3 showed the greatest PM, and was significantly different from groups 2 to 6 (p < 0.05). Group 6 showed the lowest PM. Pretreatment with Er:YAG with or without adhesive led to less PM than bur pretreatment. There were no significant differences among groups in PUA.

Conclusions: Conventional acid etching provided a similar degree of occlusal seal in teeth with fluorosis compared to those pretreated with a bur or Er:YAG laser. Pretreatment of pits and fissures with Er:YAG in teeth with fluorosis may be an alternative method before fissure sealant application.

No MeSH data available.


Related in: MedlinePlus