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In vitro cytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay.

Khedmat S, Dehghan S, Hadjati J, Masoumi F, Nekoofar MH, Dummer PM - Restor Dent Endod (2014)

Bottom Line: In all groups, the viability of monocytes significantly improved with increasing storage time regardless of the incubation time (p < 0.001).After 24 hr of incubation, there was no significant difference between the materials regarding monocyte viability.Mixing ProRoot MTA with PBS in place of distilled water had no effect on its biocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran. ; Department of Endodontics, Dental School, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Objectives: This study was performed to evaluate the cytotoxicity of four calcium silicate-based endodontic cements at different storage times after mixing.

Materials and methods: Capillary tubes were filled with Biodentine (Septodont), Calcium Enriched Mixture (CEM cement, BioniqueDent), Tech Biosealer Endo (Tech Biosealer) and ProRoot MTA (Dentsply Tulsa Dental). Empty tubes and tubes containing Dycal were used as negative and positive control groups respectively. Filled capillary tubes were kept in 0.2 mL microtubes and incubated at 37℃. Each material was divided into 3 groups for testing at intervals of 24 hr, 7 day and 28 day after mixing. Human monocytes were isolated from peripheral blood mononuclear cells and cocultered with 24 hr, 7 day and 28 day samples of different materials for 24 and 48 hr. Cell viability was evaluated using an MTT assay.

Results: In all groups, the viability of monocytes significantly improved with increasing storage time regardless of the incubation time (p < 0.001). After 24 hr of incubation, there was no significant difference between the materials regarding monocyte viability. However, at 48 hr of incubation, ProRoot MTA and Biodentine were less cytotoxic than CEM cement and Biosealer (p < 0.01).

Conclusions: Biodentine and ProRoot MTA had similar biocompatibility. Mixing ProRoot MTA with PBS in place of distilled water had no effect on its biocompatibility. Biosealer and CEM cement after 48 hr of incubation were significantly more cytotoxic to on monocyte cells compared to ProRoot MTA and Biodentine.

No MeSH data available.


Related in: MedlinePlus

The metabolic activity of human monocytes in different experimental groups and storage times. (a) After 24 hours incubation time; (b) After 48 hours incubation time. MTA, Mineral trioxide aggregate; CEM, Calcium enriched mixture; DW, Distilled water; PBS, Phosphate buffered saline.
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Figure 1: The metabolic activity of human monocytes in different experimental groups and storage times. (a) After 24 hours incubation time; (b) After 48 hours incubation time. MTA, Mineral trioxide aggregate; CEM, Calcium enriched mixture; DW, Distilled water; PBS, Phosphate buffered saline.

Mentions: Cell viability of human monocytes after 24 and 48 hours incubation with control and test groups at three storage times (24 hours, 7 days and 28 days after mixing of the materials) are shown in figure 1. There was no significant difference in monocytes viability amongst the test groups after 24 hours of incubation at three storage time (24 hours, 7 days and 28 days after mixing, Figure 1a). However, at 48 hours of incubation, monocytes cocultered in the presence of ProRoot MTA (with DW and/or PBS) and Biodentine had a significantly greater percentage of viability than Biosealer and CEM cement groups (p < 0.001) (Figure 1b). No significant difference was seen between Biodentine and ProRoot MTA when mixed with DW or PBS. In addition, there was no significant difference between Biosealer and CEM cement specimens in this incubation period.


In vitro cytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay.

Khedmat S, Dehghan S, Hadjati J, Masoumi F, Nekoofar MH, Dummer PM - Restor Dent Endod (2014)

The metabolic activity of human monocytes in different experimental groups and storage times. (a) After 24 hours incubation time; (b) After 48 hours incubation time. MTA, Mineral trioxide aggregate; CEM, Calcium enriched mixture; DW, Distilled water; PBS, Phosphate buffered saline.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125577&req=5

Figure 1: The metabolic activity of human monocytes in different experimental groups and storage times. (a) After 24 hours incubation time; (b) After 48 hours incubation time. MTA, Mineral trioxide aggregate; CEM, Calcium enriched mixture; DW, Distilled water; PBS, Phosphate buffered saline.
Mentions: Cell viability of human monocytes after 24 and 48 hours incubation with control and test groups at three storage times (24 hours, 7 days and 28 days after mixing of the materials) are shown in figure 1. There was no significant difference in monocytes viability amongst the test groups after 24 hours of incubation at three storage time (24 hours, 7 days and 28 days after mixing, Figure 1a). However, at 48 hours of incubation, monocytes cocultered in the presence of ProRoot MTA (with DW and/or PBS) and Biodentine had a significantly greater percentage of viability than Biosealer and CEM cement groups (p < 0.001) (Figure 1b). No significant difference was seen between Biodentine and ProRoot MTA when mixed with DW or PBS. In addition, there was no significant difference between Biosealer and CEM cement specimens in this incubation period.

Bottom Line: In all groups, the viability of monocytes significantly improved with increasing storage time regardless of the incubation time (p < 0.001).After 24 hr of incubation, there was no significant difference between the materials regarding monocyte viability.Mixing ProRoot MTA with PBS in place of distilled water had no effect on its biocompatibility.

View Article: PubMed Central - PubMed

Affiliation: Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran. ; Department of Endodontics, Dental School, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Objectives: This study was performed to evaluate the cytotoxicity of four calcium silicate-based endodontic cements at different storage times after mixing.

Materials and methods: Capillary tubes were filled with Biodentine (Septodont), Calcium Enriched Mixture (CEM cement, BioniqueDent), Tech Biosealer Endo (Tech Biosealer) and ProRoot MTA (Dentsply Tulsa Dental). Empty tubes and tubes containing Dycal were used as negative and positive control groups respectively. Filled capillary tubes were kept in 0.2 mL microtubes and incubated at 37℃. Each material was divided into 3 groups for testing at intervals of 24 hr, 7 day and 28 day after mixing. Human monocytes were isolated from peripheral blood mononuclear cells and cocultered with 24 hr, 7 day and 28 day samples of different materials for 24 and 48 hr. Cell viability was evaluated using an MTT assay.

Results: In all groups, the viability of monocytes significantly improved with increasing storage time regardless of the incubation time (p < 0.001). After 24 hr of incubation, there was no significant difference between the materials regarding monocyte viability. However, at 48 hr of incubation, ProRoot MTA and Biodentine were less cytotoxic than CEM cement and Biosealer (p < 0.01).

Conclusions: Biodentine and ProRoot MTA had similar biocompatibility. Mixing ProRoot MTA with PBS in place of distilled water had no effect on its biocompatibility. Biosealer and CEM cement after 48 hr of incubation were significantly more cytotoxic to on monocyte cells compared to ProRoot MTA and Biodentine.

No MeSH data available.


Related in: MedlinePlus