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Microvesicle-associated microRNA expression is altered upon particulate matter exposure in healthy workers and in A549 cells.

Bollati V, Angelici L, Rizzo G, Pergoli L, Rota F, Hoxha M, Nordio F, Bonzini M, Tarantini L, Cantone L, Pesatori AC, Apostoli P, Baccarelli AA, Bertazzi PA - J Appl Toxicol (2014)

Bottom Line: We measured the expression of 88 MV-associated miRNAs by real-time polymerase chain reaction.MiR-302c was expressed neither from A549 cells nor in reference lung RNA.These results suggest novel PM-activated molecular mechanisms that may mediate the effects of air pollution and could lead to the identification of new diagnostic and therapeutic interventions.

View Article: PubMed Central - PubMed

Affiliation: Center of Molecular and Genetic Epidemiology, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan, Italy; Epidemiology Unit, Fondazione Cà Granda IRCCS Ospedale Maggiore Policlinico, Milan, Italy.

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miR-128 expression in microvesicles purified from culture media of A549 cells treated with increasing doses of PM10 at different times.
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fig03: miR-128 expression in microvesicles purified from culture media of A549 cells treated with increasing doses of PM10 at different times.

Mentions: We determined by quantitative RT-PCR the expression levels of miR-302c and miR-128 in MVs purified from culture media of PM-treated A549 cells. MiR-128 was significantly upregulated in MVs from treated cells (Fig.3). The upregulation was dose-dependent after 6 h (P = 0.030), 24 h (P = 0.025) and 48 h (P = 0.010) of treatment. No dose dependence was observed at the earliest time point (2 h of treatment; P = 0.338). MiR-302c was undetectable in MVs from the culture media of either PM-treated or untreated cells. MiR-302c was not detectable in A549 cells or in lung reference RNA (First Choice Human Lung Total RNA; Ambion, Life Technologies, CA, USA) (data not shown).


Microvesicle-associated microRNA expression is altered upon particulate matter exposure in healthy workers and in A549 cells.

Bollati V, Angelici L, Rizzo G, Pergoli L, Rota F, Hoxha M, Nordio F, Bonzini M, Tarantini L, Cantone L, Pesatori AC, Apostoli P, Baccarelli AA, Bertazzi PA - J Appl Toxicol (2014)

miR-128 expression in microvesicles purified from culture media of A549 cells treated with increasing doses of PM10 at different times.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125569&req=5

fig03: miR-128 expression in microvesicles purified from culture media of A549 cells treated with increasing doses of PM10 at different times.
Mentions: We determined by quantitative RT-PCR the expression levels of miR-302c and miR-128 in MVs purified from culture media of PM-treated A549 cells. MiR-128 was significantly upregulated in MVs from treated cells (Fig.3). The upregulation was dose-dependent after 6 h (P = 0.030), 24 h (P = 0.025) and 48 h (P = 0.010) of treatment. No dose dependence was observed at the earliest time point (2 h of treatment; P = 0.338). MiR-302c was undetectable in MVs from the culture media of either PM-treated or untreated cells. MiR-302c was not detectable in A549 cells or in lung reference RNA (First Choice Human Lung Total RNA; Ambion, Life Technologies, CA, USA) (data not shown).

Bottom Line: We measured the expression of 88 MV-associated miRNAs by real-time polymerase chain reaction.MiR-302c was expressed neither from A549 cells nor in reference lung RNA.These results suggest novel PM-activated molecular mechanisms that may mediate the effects of air pollution and could lead to the identification of new diagnostic and therapeutic interventions.

View Article: PubMed Central - PubMed

Affiliation: Center of Molecular and Genetic Epidemiology, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan, Italy; Epidemiology Unit, Fondazione Cà Granda IRCCS Ospedale Maggiore Policlinico, Milan, Italy.

Show MeSH
Related in: MedlinePlus