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Microvesicle-associated microRNA expression is altered upon particulate matter exposure in healthy workers and in A549 cells.

Bollati V, Angelici L, Rizzo G, Pergoli L, Rota F, Hoxha M, Nordio F, Bonzini M, Tarantini L, Cantone L, Pesatori AC, Apostoli P, Baccarelli AA, Bertazzi PA - J Appl Toxicol (2014)

Bottom Line: We measured the expression of 88 MV-associated miRNAs by real-time polymerase chain reaction.MiR-302c was expressed neither from A549 cells nor in reference lung RNA.These results suggest novel PM-activated molecular mechanisms that may mediate the effects of air pollution and could lead to the identification of new diagnostic and therapeutic interventions.

View Article: PubMed Central - PubMed

Affiliation: Center of Molecular and Genetic Epidemiology, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan, Italy; Epidemiology Unit, Fondazione Cà Granda IRCCS Ospedale Maggiore Policlinico, Milan, Italy.

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Growth profile of A549 cells treated with increasing doses of PM10.
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fig02: Growth profile of A549 cells treated with increasing doses of PM10.

Mentions: We examined the effect of increasing PM doses on the proliferation of A549 cells with the xCELLigence detection system (Roche Diagnostic, Basel, Switzerland) and found a significant dose-dependent decrease in cell proliferation over time. Each PM concentration was tested 12 times. The average (means ± SD) doubling times were 22.5 ± 1.7, 17.4 ± 1.3, 17.4 ± 0.9, 16.6 ± 0.8, 15.8 ± 1.1 and 16.3 ± 0.8 h for cells treated with 250, 125, 62, 31, 15 and 0 µg ml–1 PM, respectively (Fig.2).


Microvesicle-associated microRNA expression is altered upon particulate matter exposure in healthy workers and in A549 cells.

Bollati V, Angelici L, Rizzo G, Pergoli L, Rota F, Hoxha M, Nordio F, Bonzini M, Tarantini L, Cantone L, Pesatori AC, Apostoli P, Baccarelli AA, Bertazzi PA - J Appl Toxicol (2014)

Growth profile of A549 cells treated with increasing doses of PM10.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125569&req=5

fig02: Growth profile of A549 cells treated with increasing doses of PM10.
Mentions: We examined the effect of increasing PM doses on the proliferation of A549 cells with the xCELLigence detection system (Roche Diagnostic, Basel, Switzerland) and found a significant dose-dependent decrease in cell proliferation over time. Each PM concentration was tested 12 times. The average (means ± SD) doubling times were 22.5 ± 1.7, 17.4 ± 1.3, 17.4 ± 0.9, 16.6 ± 0.8, 15.8 ± 1.1 and 16.3 ± 0.8 h for cells treated with 250, 125, 62, 31, 15 and 0 µg ml–1 PM, respectively (Fig.2).

Bottom Line: We measured the expression of 88 MV-associated miRNAs by real-time polymerase chain reaction.MiR-302c was expressed neither from A549 cells nor in reference lung RNA.These results suggest novel PM-activated molecular mechanisms that may mediate the effects of air pollution and could lead to the identification of new diagnostic and therapeutic interventions.

View Article: PubMed Central - PubMed

Affiliation: Center of Molecular and Genetic Epidemiology, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan, Italy; Epidemiology Unit, Fondazione Cà Granda IRCCS Ospedale Maggiore Policlinico, Milan, Italy.

Show MeSH
Related in: MedlinePlus