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Microvesicle-associated microRNA expression is altered upon particulate matter exposure in healthy workers and in A549 cells.

Bollati V, Angelici L, Rizzo G, Pergoli L, Rota F, Hoxha M, Nordio F, Bonzini M, Tarantini L, Cantone L, Pesatori AC, Apostoli P, Baccarelli AA, Bertazzi PA - J Appl Toxicol (2014)

Bottom Line: We measured the expression of 88 MV-associated miRNAs by real-time polymerase chain reaction.MiR-302c was expressed neither from A549 cells nor in reference lung RNA.These results suggest novel PM-activated molecular mechanisms that may mediate the effects of air pollution and could lead to the identification of new diagnostic and therapeutic interventions.

View Article: PubMed Central - PubMed

Affiliation: Center of Molecular and Genetic Epidemiology, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan, Italy; Epidemiology Unit, Fondazione Cà Granda IRCCS Ospedale Maggiore Policlinico, Milan, Italy.

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Volcano plot representing differential microRNA expression in plasma microvesicles of steel plant workers pre- and postexposure.
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fig01: Volcano plot representing differential microRNA expression in plasma microvesicles of steel plant workers pre- and postexposure.

Mentions: We compared the miRNA expression levels of the 55 baseline blood samples to those of the 55 paired postexposure blood samples. We identified six miRNAs that showed significant differential expressions (Table 1). The most notable were miR-302c and miR-128 that were upregulated 13.95- and 5.62-fold, respectively (P < 0.001). MiR-28-3p, let-7 g, miR-125a-5p and miR-181a were upregulated 3.64-fold (P = 0.01), 2.27-fold (P = 0.02), 2.98-fold (P = 0.04) and 1.72-fold (P = 0.02), respectively (Fig.1). When the FDR method was applied to account for multiple testing, both miR-302c and miR-128 passed the FDR threshold of < 0.10, with FDRs of 0.06 and 0.05, respectively (Table 1).


Microvesicle-associated microRNA expression is altered upon particulate matter exposure in healthy workers and in A549 cells.

Bollati V, Angelici L, Rizzo G, Pergoli L, Rota F, Hoxha M, Nordio F, Bonzini M, Tarantini L, Cantone L, Pesatori AC, Apostoli P, Baccarelli AA, Bertazzi PA - J Appl Toxicol (2014)

Volcano plot representing differential microRNA expression in plasma microvesicles of steel plant workers pre- and postexposure.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125569&req=5

fig01: Volcano plot representing differential microRNA expression in plasma microvesicles of steel plant workers pre- and postexposure.
Mentions: We compared the miRNA expression levels of the 55 baseline blood samples to those of the 55 paired postexposure blood samples. We identified six miRNAs that showed significant differential expressions (Table 1). The most notable were miR-302c and miR-128 that were upregulated 13.95- and 5.62-fold, respectively (P < 0.001). MiR-28-3p, let-7 g, miR-125a-5p and miR-181a were upregulated 3.64-fold (P = 0.01), 2.27-fold (P = 0.02), 2.98-fold (P = 0.04) and 1.72-fold (P = 0.02), respectively (Fig.1). When the FDR method was applied to account for multiple testing, both miR-302c and miR-128 passed the FDR threshold of < 0.10, with FDRs of 0.06 and 0.05, respectively (Table 1).

Bottom Line: We measured the expression of 88 MV-associated miRNAs by real-time polymerase chain reaction.MiR-302c was expressed neither from A549 cells nor in reference lung RNA.These results suggest novel PM-activated molecular mechanisms that may mediate the effects of air pollution and could lead to the identification of new diagnostic and therapeutic interventions.

View Article: PubMed Central - PubMed

Affiliation: Center of Molecular and Genetic Epidemiology, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan, Italy; Epidemiology Unit, Fondazione Cà Granda IRCCS Ospedale Maggiore Policlinico, Milan, Italy.

Show MeSH
Related in: MedlinePlus