Operons are a conserved feature of nematode genomes.
Bottom Line: The organization of genes into operons, clusters of genes that are co-transcribed to produce polycistronic pre-mRNAs, is a trait found in a wide range of eukaryotic groups, including multiple animal phyla.We have nevertheless identified putative operons conserved between Enoplea and Chromadorea.Our data suggest that operons and "spliced leader" (SL) trans-splicing predate the radiation of the nematode phylum, an inference which is supported by the phylogenetic profile of proteins known to be involved in nematode SL trans-splicing.
Affiliation: School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom email@example.com.Show MeSH
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Mentions: The analysis of one of the two SL trans-spliced T. muris mRNAs (GenBank accession no. FF145866) led to the identification of a putative operon conserved between multiple nematodes (Figure 3A). The two genes contained in these putative operons have C. elegans homologs, cpt-2 and nuaf-3, respectively, which are in the same operon (CEOP4424) (Figure 3A), although there is an additional gene, prx-14, located between these genes in CEOP4424 that is not present in the putative homologous T. spiralis operon. Examination of the genomic organization of the homologous genes in a selection of nematode species confirmed the evolutionary conservation of the synteny of the cpt-2 and nuaf-3 homologs (Figure 3A). This analysis also showed that insertion of prx-14 into the cpt-2∼nuaf-3 operon was a relatively recent event, since it is present only in C. elegans and other closely related Caenorhabditis species. We also find, based on the head-to-tail organization and spacing between coding regions, that there is variation in the composition of both operons in different species, and these genes in R. culicivorax are not in operons.
Affiliation: School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom firstname.lastname@example.org.