The role of Exo1p exonuclease in DNA end resection to generate gene conversion tracts in Saccharomyces cerevisiae.
Bottom Line: In accordance with this expectation, gene conversion tract lengths associated with spontaneous crossovers in exo1 strains were reduced about twofold relative to wild type.For UV-induced events, conversion tract lengths associated with crossovers were also shorter for the exo1 strain than for the wild-type strain (3.2 and 7.6 kb, respectively).Unexpectedly, however, the lengths of conversion tracts that were unassociated with crossovers were longer in the exo1 strain than in the wild-type strain (6.2 and 4.8 kb, respectively).
Affiliation: Department of Molecular Genetics and Microbiology and University Program in Genetics and Genomics, Duke University Medical Center, Durham, North Carolina 27710.Show MeSH
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Mentions: The frequency and map positions of recombination events were examined in a diploid heterozygous for ∼55,000 single-nucleotide polymorphisms (SNPs) distributed throughout the genome (Lee et al. 2009). Crossovers and gene conversion events occurring between homologs can be detected as loss of heterozygosity (LOH), using oligonucleotide-containing microarrays (described further below). Some of the patterns of LOH observed in previous studies (St. Charles et al. 2012; St. Charles and Petes 2013; Yin and Petes 2013) are shown in Figure 1.
Affiliation: Department of Molecular Genetics and Microbiology and University Program in Genetics and Genomics, Duke University Medical Center, Durham, North Carolina 27710.