Limits...
Hydroxyproline, a serum biomarker candidate for gastric ulcer in rats: a comparison study of metabolic analysis of gastric ulcer models induced by ethanol, stress, and aspirin.

Takeuchi K, Ohishi M, Endo K, Suzumura K, Naraoka H, Ohata T, Seki J, Miyamae Y, Honma M, Soga T - Biomark Insights (2014)

Bottom Line: Gastrointestinal symptoms are a common manifestation of adverse drug effects.Biomarkers for these side effects have not been identified and ulcers are now only detectable by endoscopy.While extrapolation to humans requires further study, hydroxyproline can be a new serum biomarker of gastric injury regardless of cause.

View Article: PubMed Central - PubMed

Affiliation: Drug Safety Research Laboratories, Astellas Pharma Inc., Yodogawa-ku, Osaka, Japan.

ABSTRACT
Gastrointestinal symptoms are a common manifestation of adverse drug effects. Non-steroid anti-inflammatory drugs (NSAIDs) are widely prescribed drugs that induce the serious side effect of gastric mucosal ulceration. Biomarkers for these side effects have not been identified and ulcers are now only detectable by endoscopy. We previously identified five metabolites as biomarker candidates for NSAID-induced gastric ulcer using capillary electrophoresis-mass spectrometry (CE-MS)-based metabolomic analysis of serum and stomach from rats. Here, to clarify mechanism of changes and limitations of indications of biomarker candidates, we performed CE-MS-based metabolomic profiling in stomach and serum from rats with gastric ulcers induced by ethanol, stress, and aspirin. The results suggest that a decrease in hydroxyproline reflects the induction of gastric injury and may be useful in identifying gastric ulcer induced by multiple causes. While extrapolation to humans requires further study, hydroxyproline can be a new serum biomarker of gastric injury regardless of cause.

No MeSH data available.


Related in: MedlinePlus

Levels of hydroxyproline, putrescine, and N8-acetylspermidine in serum.Notes: Data are reported as means ± standard deviation of four or eight animals per group. Asterisks indicate statistically significant differences: **P < 0.01, *P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4125369&req=5

f4-bmi-9-2014-061: Levels of hydroxyproline, putrescine, and N8-acetylspermidine in serum.Notes: Data are reported as means ± standard deviation of four or eight animals per group. Asterisks indicate statistically significant differences: **P < 0.01, *P < 0.05.

Mentions: Serum concentrations of hydroxyproline and putrescine are shown in Figure 4. Statistical analysis of quantitative differences between groups showed decreases in the levels of hydroxyproline in all models. In contrast, changes in the levels of putrescine were not observed in all models. Further, the serum concentration of N8-acetylspermidine was not determined because it was below detection limits.


Hydroxyproline, a serum biomarker candidate for gastric ulcer in rats: a comparison study of metabolic analysis of gastric ulcer models induced by ethanol, stress, and aspirin.

Takeuchi K, Ohishi M, Endo K, Suzumura K, Naraoka H, Ohata T, Seki J, Miyamae Y, Honma M, Soga T - Biomark Insights (2014)

Levels of hydroxyproline, putrescine, and N8-acetylspermidine in serum.Notes: Data are reported as means ± standard deviation of four or eight animals per group. Asterisks indicate statistically significant differences: **P < 0.01, *P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4125369&req=5

f4-bmi-9-2014-061: Levels of hydroxyproline, putrescine, and N8-acetylspermidine in serum.Notes: Data are reported as means ± standard deviation of four or eight animals per group. Asterisks indicate statistically significant differences: **P < 0.01, *P < 0.05.
Mentions: Serum concentrations of hydroxyproline and putrescine are shown in Figure 4. Statistical analysis of quantitative differences between groups showed decreases in the levels of hydroxyproline in all models. In contrast, changes in the levels of putrescine were not observed in all models. Further, the serum concentration of N8-acetylspermidine was not determined because it was below detection limits.

Bottom Line: Gastrointestinal symptoms are a common manifestation of adverse drug effects.Biomarkers for these side effects have not been identified and ulcers are now only detectable by endoscopy.While extrapolation to humans requires further study, hydroxyproline can be a new serum biomarker of gastric injury regardless of cause.

View Article: PubMed Central - PubMed

Affiliation: Drug Safety Research Laboratories, Astellas Pharma Inc., Yodogawa-ku, Osaka, Japan.

ABSTRACT
Gastrointestinal symptoms are a common manifestation of adverse drug effects. Non-steroid anti-inflammatory drugs (NSAIDs) are widely prescribed drugs that induce the serious side effect of gastric mucosal ulceration. Biomarkers for these side effects have not been identified and ulcers are now only detectable by endoscopy. We previously identified five metabolites as biomarker candidates for NSAID-induced gastric ulcer using capillary electrophoresis-mass spectrometry (CE-MS)-based metabolomic analysis of serum and stomach from rats. Here, to clarify mechanism of changes and limitations of indications of biomarker candidates, we performed CE-MS-based metabolomic profiling in stomach and serum from rats with gastric ulcers induced by ethanol, stress, and aspirin. The results suggest that a decrease in hydroxyproline reflects the induction of gastric injury and may be useful in identifying gastric ulcer induced by multiple causes. While extrapolation to humans requires further study, hydroxyproline can be a new serum biomarker of gastric injury regardless of cause.

No MeSH data available.


Related in: MedlinePlus