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Proteomic analysis of Apis cerana and Apis mellifera larvae fed with heterospecific royal jelly and by CSBV challenge.

Zhang Y, Zhang G, Huang X, Han R - PLoS ONE (2014)

Bottom Line: Heterospecific royal jelly (RJ) breeding in two honeybee species may result in morphological and genetic modification.In Ac larvae, 6 differential expression proteins were identified from heterospecific RJ breeding only, 21 differential expression proteins from CSBV challenge only and 7 differential expression proteins from heterospecific RJ breeding plus CSBV challenge.In Am larvae, 17 differential expression proteins were identified from heterospecific RJ breeding only, 26 differential expression proteins from CSBV challenge only and 24 differential expression proteins from heterospecific RJ breeding plus CSBV challenge.

View Article: PubMed Central - PubMed

Affiliation: Guangdong Entomological Institute, Guangzhou, China.

ABSTRACT
Chinese honeybee Apis cerana (Ac) is one of the major Asian honeybee species for local apiculture. However, Ac is frequently damaged by Chinese sacbrood virus (CSBV), whereas Apis mellifera (Am) is usually resistant to it. Heterospecific royal jelly (RJ) breeding in two honeybee species may result in morphological and genetic modification. Nevertheless, knowledge on the resistant mechanism of Am to this deadly disease is still unknown. In the present study, heterospecific RJ breeding was conducted to determine the effects of food change on the larval mortality after CSBV infection at early larval stage. 2-DE and MALDI-TOF/TOF MS proteomic technology was employed to unravel the molecular event of the bees under heterospecific RJ breeding and CSBV challenge. The change of Ac larval food from RJC to RJM could enhance the bee resistance to CSBV. The mortality rate of Ac larvae after CSBV infection was much higher when the larvae were fed with RJC compared with the larvae fed with RJM. There were 101 proteins with altered expressions after heterospecific RJ breeding and viral infection. In Ac larvae, 6 differential expression proteins were identified from heterospecific RJ breeding only, 21 differential expression proteins from CSBV challenge only and 7 differential expression proteins from heterospecific RJ breeding plus CSBV challenge. In Am larvae, 17 differential expression proteins were identified from heterospecific RJ breeding only, 26 differential expression proteins from CSBV challenge only and 24 differential expression proteins from heterospecific RJ breeding plus CSBV challenge. The RJM may protect Ac larvae from CSBV infection, probably by activating the genes in energy metabolism pathways, antioxidation and ubiquitin-proteasome system. The present results, for the first time, comprehensively descript the molecular events of the viral infection of Ac and Am after heterospecific RJ breeding and are potentially useful for establishing CSBV resistant populations of Ac for apiculture.

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Protein-protein interaction networks of identified differentially expressed proteins from nutritional crossbreeding and CSBV challenge were constructed by Search Tool for the Retrieval of Interacting Genes (STRING).Lines represent the existence of the different types of evidence used in predicting the associations. A red line indicates the presence of fusion evidence; a green line, neighborhood evidence; a blue line, co-occurrence evidence; a purple line, experimental evidence; a yellow line, text mining evidence; a light blue line, database evidence; a black line, coexpression evidence. PFPs: Predicted Functional Partners. A: 3-day Ac larvae fed with RJM. B: 3-day Ac larvae fed with RJC, and infected with CSBV. C: 3-day Ac larvae fed with RJM, and infected with CSBV. D: 3-day Am larvae fed with RJC. E: 3-day Am larvae fed with RJM, and infected with CSBV; F: 3-day Am larvae fed with RJC, and infected with CSBV.
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pone-0102663-g006: Protein-protein interaction networks of identified differentially expressed proteins from nutritional crossbreeding and CSBV challenge were constructed by Search Tool for the Retrieval of Interacting Genes (STRING).Lines represent the existence of the different types of evidence used in predicting the associations. A red line indicates the presence of fusion evidence; a green line, neighborhood evidence; a blue line, co-occurrence evidence; a purple line, experimental evidence; a yellow line, text mining evidence; a light blue line, database evidence; a black line, coexpression evidence. PFPs: Predicted Functional Partners. A: 3-day Ac larvae fed with RJM. B: 3-day Ac larvae fed with RJC, and infected with CSBV. C: 3-day Ac larvae fed with RJM, and infected with CSBV. D: 3-day Am larvae fed with RJC. E: 3-day Am larvae fed with RJM, and infected with CSBV; F: 3-day Am larvae fed with RJC, and infected with CSBV.

Mentions: Proteins function jointly in a living cell through networks by forming protein- protein interactions (PPI), modifications and regulation of expression relationships. Of all the identified proteins in this study, 107 proteins with the established functions based on Drosophila melanogaster were selected and their PPI mapping was constructed using STRING. The result showed that all the treats almost divided into two distinct PPI clusters with several others operating without connectivity. The first cluster primarily consisted of cytoskeletal proteins, while the second cluster mainly consisted of proteins involved in metabolism (Figure 6).


Proteomic analysis of Apis cerana and Apis mellifera larvae fed with heterospecific royal jelly and by CSBV challenge.

Zhang Y, Zhang G, Huang X, Han R - PLoS ONE (2014)

Protein-protein interaction networks of identified differentially expressed proteins from nutritional crossbreeding and CSBV challenge were constructed by Search Tool for the Retrieval of Interacting Genes (STRING).Lines represent the existence of the different types of evidence used in predicting the associations. A red line indicates the presence of fusion evidence; a green line, neighborhood evidence; a blue line, co-occurrence evidence; a purple line, experimental evidence; a yellow line, text mining evidence; a light blue line, database evidence; a black line, coexpression evidence. PFPs: Predicted Functional Partners. A: 3-day Ac larvae fed with RJM. B: 3-day Ac larvae fed with RJC, and infected with CSBV. C: 3-day Ac larvae fed with RJM, and infected with CSBV. D: 3-day Am larvae fed with RJC. E: 3-day Am larvae fed with RJM, and infected with CSBV; F: 3-day Am larvae fed with RJC, and infected with CSBV.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125304&req=5

pone-0102663-g006: Protein-protein interaction networks of identified differentially expressed proteins from nutritional crossbreeding and CSBV challenge were constructed by Search Tool for the Retrieval of Interacting Genes (STRING).Lines represent the existence of the different types of evidence used in predicting the associations. A red line indicates the presence of fusion evidence; a green line, neighborhood evidence; a blue line, co-occurrence evidence; a purple line, experimental evidence; a yellow line, text mining evidence; a light blue line, database evidence; a black line, coexpression evidence. PFPs: Predicted Functional Partners. A: 3-day Ac larvae fed with RJM. B: 3-day Ac larvae fed with RJC, and infected with CSBV. C: 3-day Ac larvae fed with RJM, and infected with CSBV. D: 3-day Am larvae fed with RJC. E: 3-day Am larvae fed with RJM, and infected with CSBV; F: 3-day Am larvae fed with RJC, and infected with CSBV.
Mentions: Proteins function jointly in a living cell through networks by forming protein- protein interactions (PPI), modifications and regulation of expression relationships. Of all the identified proteins in this study, 107 proteins with the established functions based on Drosophila melanogaster were selected and their PPI mapping was constructed using STRING. The result showed that all the treats almost divided into two distinct PPI clusters with several others operating without connectivity. The first cluster primarily consisted of cytoskeletal proteins, while the second cluster mainly consisted of proteins involved in metabolism (Figure 6).

Bottom Line: Heterospecific royal jelly (RJ) breeding in two honeybee species may result in morphological and genetic modification.In Ac larvae, 6 differential expression proteins were identified from heterospecific RJ breeding only, 21 differential expression proteins from CSBV challenge only and 7 differential expression proteins from heterospecific RJ breeding plus CSBV challenge.In Am larvae, 17 differential expression proteins were identified from heterospecific RJ breeding only, 26 differential expression proteins from CSBV challenge only and 24 differential expression proteins from heterospecific RJ breeding plus CSBV challenge.

View Article: PubMed Central - PubMed

Affiliation: Guangdong Entomological Institute, Guangzhou, China.

ABSTRACT
Chinese honeybee Apis cerana (Ac) is one of the major Asian honeybee species for local apiculture. However, Ac is frequently damaged by Chinese sacbrood virus (CSBV), whereas Apis mellifera (Am) is usually resistant to it. Heterospecific royal jelly (RJ) breeding in two honeybee species may result in morphological and genetic modification. Nevertheless, knowledge on the resistant mechanism of Am to this deadly disease is still unknown. In the present study, heterospecific RJ breeding was conducted to determine the effects of food change on the larval mortality after CSBV infection at early larval stage. 2-DE and MALDI-TOF/TOF MS proteomic technology was employed to unravel the molecular event of the bees under heterospecific RJ breeding and CSBV challenge. The change of Ac larval food from RJC to RJM could enhance the bee resistance to CSBV. The mortality rate of Ac larvae after CSBV infection was much higher when the larvae were fed with RJC compared with the larvae fed with RJM. There were 101 proteins with altered expressions after heterospecific RJ breeding and viral infection. In Ac larvae, 6 differential expression proteins were identified from heterospecific RJ breeding only, 21 differential expression proteins from CSBV challenge only and 7 differential expression proteins from heterospecific RJ breeding plus CSBV challenge. In Am larvae, 17 differential expression proteins were identified from heterospecific RJ breeding only, 26 differential expression proteins from CSBV challenge only and 24 differential expression proteins from heterospecific RJ breeding plus CSBV challenge. The RJM may protect Ac larvae from CSBV infection, probably by activating the genes in energy metabolism pathways, antioxidation and ubiquitin-proteasome system. The present results, for the first time, comprehensively descript the molecular events of the viral infection of Ac and Am after heterospecific RJ breeding and are potentially useful for establishing CSBV resistant populations of Ac for apiculture.

Show MeSH
Related in: MedlinePlus