Limits...
Proteomic analysis of Apis cerana and Apis mellifera larvae fed with heterospecific royal jelly and by CSBV challenge.

Zhang Y, Zhang G, Huang X, Han R - PLoS ONE (2014)

Bottom Line: Heterospecific royal jelly (RJ) breeding in two honeybee species may result in morphological and genetic modification.In Ac larvae, 6 differential expression proteins were identified from heterospecific RJ breeding only, 21 differential expression proteins from CSBV challenge only and 7 differential expression proteins from heterospecific RJ breeding plus CSBV challenge.In Am larvae, 17 differential expression proteins were identified from heterospecific RJ breeding only, 26 differential expression proteins from CSBV challenge only and 24 differential expression proteins from heterospecific RJ breeding plus CSBV challenge.

View Article: PubMed Central - PubMed

Affiliation: Guangdong Entomological Institute, Guangzhou, China.

ABSTRACT
Chinese honeybee Apis cerana (Ac) is one of the major Asian honeybee species for local apiculture. However, Ac is frequently damaged by Chinese sacbrood virus (CSBV), whereas Apis mellifera (Am) is usually resistant to it. Heterospecific royal jelly (RJ) breeding in two honeybee species may result in morphological and genetic modification. Nevertheless, knowledge on the resistant mechanism of Am to this deadly disease is still unknown. In the present study, heterospecific RJ breeding was conducted to determine the effects of food change on the larval mortality after CSBV infection at early larval stage. 2-DE and MALDI-TOF/TOF MS proteomic technology was employed to unravel the molecular event of the bees under heterospecific RJ breeding and CSBV challenge. The change of Ac larval food from RJC to RJM could enhance the bee resistance to CSBV. The mortality rate of Ac larvae after CSBV infection was much higher when the larvae were fed with RJC compared with the larvae fed with RJM. There were 101 proteins with altered expressions after heterospecific RJ breeding and viral infection. In Ac larvae, 6 differential expression proteins were identified from heterospecific RJ breeding only, 21 differential expression proteins from CSBV challenge only and 7 differential expression proteins from heterospecific RJ breeding plus CSBV challenge. In Am larvae, 17 differential expression proteins were identified from heterospecific RJ breeding only, 26 differential expression proteins from CSBV challenge only and 24 differential expression proteins from heterospecific RJ breeding plus CSBV challenge. The RJM may protect Ac larvae from CSBV infection, probably by activating the genes in energy metabolism pathways, antioxidation and ubiquitin-proteasome system. The present results, for the first time, comprehensively descript the molecular events of the viral infection of Ac and Am after heterospecific RJ breeding and are potentially useful for establishing CSBV resistant populations of Ac for apiculture.

Show MeSH

Related in: MedlinePlus

The larval mortalities of A. cerana and A. mellifera under different treatments after 56 hours.RJC: royal jelly from A. cerana; RJM: royal jelly from A. mellifera; AC-RJC: 3-day Ac larvae fed with RJC. AC-RJM: 3-day Ac larvae fed with RJM. AC-RJC+CSBV: 3-day Ac larvae fed with RJC, and infected with CSBV. AC-RJM+CSBV: 3-day Ac larvae fed with RJM, and infected with CSBV. AM-RJC: 3-day Am larvae fed with RJC. AM-RJM: 3-day Am larvae fed with RJM. AM-RJC+CSBV: 3-day Am larvae fed with RJC, and infected with CSBV. AM-RJM+CSBV: 3-day Am larvae fed with RJM, and infected with CSBV. The data are averages from three replicates. The error bars indicate standard deviations. Bars with different letters mean significantly different (P<0.05, independent samples t test).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4125304&req=5

pone-0102663-g001: The larval mortalities of A. cerana and A. mellifera under different treatments after 56 hours.RJC: royal jelly from A. cerana; RJM: royal jelly from A. mellifera; AC-RJC: 3-day Ac larvae fed with RJC. AC-RJM: 3-day Ac larvae fed with RJM. AC-RJC+CSBV: 3-day Ac larvae fed with RJC, and infected with CSBV. AC-RJM+CSBV: 3-day Ac larvae fed with RJM, and infected with CSBV. AM-RJC: 3-day Am larvae fed with RJC. AM-RJM: 3-day Am larvae fed with RJM. AM-RJC+CSBV: 3-day Am larvae fed with RJC, and infected with CSBV. AM-RJM+CSBV: 3-day Am larvae fed with RJM, and infected with CSBV. The data are averages from three replicates. The error bars indicate standard deviations. Bars with different letters mean significantly different (P<0.05, independent samples t test).

Mentions: Experiments were carried out to examine whether RJM would enhance the resistant ability of Ac larvae to CSBV infection. The larval mortalities of the treatments after 56 h were indicated in Figure 1. The dead larvae showed the symptom of CSBV infection. Without CSBV infection, the mortalities of Ac larvae fed with RJC or RJM were not significantly different (t = 2.040, df = 4, p = 0.111; Figure 1 A); meanwhile, the mortalities of Am larvae fed with RJM or RJC were also not significantly different (t = 1.572, df = 4, p = 0.191; Figure 1 E). This indicated that the RJs did not significantly influence the mortalities of the larvae of two bee species, at least at key early larval stage.


Proteomic analysis of Apis cerana and Apis mellifera larvae fed with heterospecific royal jelly and by CSBV challenge.

Zhang Y, Zhang G, Huang X, Han R - PLoS ONE (2014)

The larval mortalities of A. cerana and A. mellifera under different treatments after 56 hours.RJC: royal jelly from A. cerana; RJM: royal jelly from A. mellifera; AC-RJC: 3-day Ac larvae fed with RJC. AC-RJM: 3-day Ac larvae fed with RJM. AC-RJC+CSBV: 3-day Ac larvae fed with RJC, and infected with CSBV. AC-RJM+CSBV: 3-day Ac larvae fed with RJM, and infected with CSBV. AM-RJC: 3-day Am larvae fed with RJC. AM-RJM: 3-day Am larvae fed with RJM. AM-RJC+CSBV: 3-day Am larvae fed with RJC, and infected with CSBV. AM-RJM+CSBV: 3-day Am larvae fed with RJM, and infected with CSBV. The data are averages from three replicates. The error bars indicate standard deviations. Bars with different letters mean significantly different (P<0.05, independent samples t test).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125304&req=5

pone-0102663-g001: The larval mortalities of A. cerana and A. mellifera under different treatments after 56 hours.RJC: royal jelly from A. cerana; RJM: royal jelly from A. mellifera; AC-RJC: 3-day Ac larvae fed with RJC. AC-RJM: 3-day Ac larvae fed with RJM. AC-RJC+CSBV: 3-day Ac larvae fed with RJC, and infected with CSBV. AC-RJM+CSBV: 3-day Ac larvae fed with RJM, and infected with CSBV. AM-RJC: 3-day Am larvae fed with RJC. AM-RJM: 3-day Am larvae fed with RJM. AM-RJC+CSBV: 3-day Am larvae fed with RJC, and infected with CSBV. AM-RJM+CSBV: 3-day Am larvae fed with RJM, and infected with CSBV. The data are averages from three replicates. The error bars indicate standard deviations. Bars with different letters mean significantly different (P<0.05, independent samples t test).
Mentions: Experiments were carried out to examine whether RJM would enhance the resistant ability of Ac larvae to CSBV infection. The larval mortalities of the treatments after 56 h were indicated in Figure 1. The dead larvae showed the symptom of CSBV infection. Without CSBV infection, the mortalities of Ac larvae fed with RJC or RJM were not significantly different (t = 2.040, df = 4, p = 0.111; Figure 1 A); meanwhile, the mortalities of Am larvae fed with RJM or RJC were also not significantly different (t = 1.572, df = 4, p = 0.191; Figure 1 E). This indicated that the RJs did not significantly influence the mortalities of the larvae of two bee species, at least at key early larval stage.

Bottom Line: Heterospecific royal jelly (RJ) breeding in two honeybee species may result in morphological and genetic modification.In Ac larvae, 6 differential expression proteins were identified from heterospecific RJ breeding only, 21 differential expression proteins from CSBV challenge only and 7 differential expression proteins from heterospecific RJ breeding plus CSBV challenge.In Am larvae, 17 differential expression proteins were identified from heterospecific RJ breeding only, 26 differential expression proteins from CSBV challenge only and 24 differential expression proteins from heterospecific RJ breeding plus CSBV challenge.

View Article: PubMed Central - PubMed

Affiliation: Guangdong Entomological Institute, Guangzhou, China.

ABSTRACT
Chinese honeybee Apis cerana (Ac) is one of the major Asian honeybee species for local apiculture. However, Ac is frequently damaged by Chinese sacbrood virus (CSBV), whereas Apis mellifera (Am) is usually resistant to it. Heterospecific royal jelly (RJ) breeding in two honeybee species may result in morphological and genetic modification. Nevertheless, knowledge on the resistant mechanism of Am to this deadly disease is still unknown. In the present study, heterospecific RJ breeding was conducted to determine the effects of food change on the larval mortality after CSBV infection at early larval stage. 2-DE and MALDI-TOF/TOF MS proteomic technology was employed to unravel the molecular event of the bees under heterospecific RJ breeding and CSBV challenge. The change of Ac larval food from RJC to RJM could enhance the bee resistance to CSBV. The mortality rate of Ac larvae after CSBV infection was much higher when the larvae were fed with RJC compared with the larvae fed with RJM. There were 101 proteins with altered expressions after heterospecific RJ breeding and viral infection. In Ac larvae, 6 differential expression proteins were identified from heterospecific RJ breeding only, 21 differential expression proteins from CSBV challenge only and 7 differential expression proteins from heterospecific RJ breeding plus CSBV challenge. In Am larvae, 17 differential expression proteins were identified from heterospecific RJ breeding only, 26 differential expression proteins from CSBV challenge only and 24 differential expression proteins from heterospecific RJ breeding plus CSBV challenge. The RJM may protect Ac larvae from CSBV infection, probably by activating the genes in energy metabolism pathways, antioxidation and ubiquitin-proteasome system. The present results, for the first time, comprehensively descript the molecular events of the viral infection of Ac and Am after heterospecific RJ breeding and are potentially useful for establishing CSBV resistant populations of Ac for apiculture.

Show MeSH
Related in: MedlinePlus