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CD16 expression on monocytes in healthy individuals but not schistosome-infected patients is positively associated with levels of parasite-specific IgG and IgG1.

Appleby LJ, Nausch N, Erskine L, Bourke CD, Rujeni N, Midzi N, Mduluza T, Mutapi F - PLoS Negl Trop Dis (2014)

Bottom Line: Human IgG1 antibody responses are associated with protection against Schistosoma haematobium infection and are now a target for schistosome vaccine development.Systemic levels of schistosome-specific anti-adult worm total IgG and IgG subclass titres were measured by ELISA in 100 individuals from an S. haematobium endemic area in Zimbabwe and, using parametric statistical methods and regression analysis, related to the levels of CD16 expression on individuals' circulating monocytes, determined via flow cytometry.Further understanding the elements of a protective immune response in schistosomiasis may aid in efforts to develop a protective vaccine against this disease.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology & Infection Research, Centre for Immunity, Infection & Evolution, School of Biological Sciences, University of Edinburgh, United Kingdom.

ABSTRACT
Human IgG1 antibody responses are associated with protection against Schistosoma haematobium infection and are now a target for schistosome vaccine development. This study aimed to investigate the relationship between total IgG and the IgG subclasses and the monocyte IgG receptor, known as FcγRIIIa or CD16, in schistosome exposed people. Systemic levels of schistosome-specific anti-adult worm total IgG and IgG subclass titres were measured by ELISA in 100 individuals from an S. haematobium endemic area in Zimbabwe and, using parametric statistical methods and regression analysis, related to the levels of CD16 expression on individuals' circulating monocytes, determined via flow cytometry. Monocyte CD16 expression rose with parasite-specific total IgG and IgG1 in healthy participants, but not in schistosome infected patients. Similar to parasite-specific IgG and IgG1, CD16 expression in healthy individuals is associated with protection against schistosome infection. This relationship indicates a mechanistic link between the innate and adaptive immune responses to helminth infection in protection against infection. Further understanding the elements of a protective immune response in schistosomiasis may aid in efforts to develop a protective vaccine against this disease.

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Total adult worm-specific IgG levels differ by age group in healthy participants but not schistosome patients.Adult worm specific (A) total IgG, (B) IgG1, (C) IgG2, (D) IgG3, (E) IgG4 responses by age group and infection status measured by ELISA. Open circles and dashed lines, healthy individuals; closed circles and solid lines, schistosome patients. Bars represent standard error of mean. Significant differences between schistosome infected and uninfected participants within age groups according to sub-group analysis are indicated by p: ** p≤0.005, *p≤0.05.
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pntd-0003049-g002: Total adult worm-specific IgG levels differ by age group in healthy participants but not schistosome patients.Adult worm specific (A) total IgG, (B) IgG1, (C) IgG2, (D) IgG3, (E) IgG4 responses by age group and infection status measured by ELISA. Open circles and dashed lines, healthy individuals; closed circles and solid lines, schistosome patients. Bars represent standard error of mean. Significant differences between schistosome infected and uninfected participants within age groups according to sub-group analysis are indicated by p: ** p≤0.005, *p≤0.05.

Mentions: The relationship between SWAP-specific total IgG, as well as the IgG subclasses and infection status dependent on age was investigated. Total IgG, IgG1, IgG2 and IgG4, but not IgG3, significantly varied with host age, with total schistosome-specific IgG levels lowest in the age of peak schistosome infection, but the adult worm-specific subclasses IgG1, IgG2 and IgG4 highest in the oldest age group (Table 3). Overall, levels of parasite-specific IgG and IgG1 varied between healthy participants compared to schistosome infected people, with infected individuals having greater antibody levels compared to uninfected individuals (Table 3). However, only for total IgG, the effects of infection status varied with host age as indicated by the significant age group-infection status interaction term (Table 3). Figure 2 demonstrates the gradual increase in levels of schistosome-specific total IgG in healthy participants, while in schistosome infected patients, total IgG levels did not change significantly with age. For total IgG, IgG1, IgG3 and IgG4, but not IgG2, the youngest age group showed significant differences in antibody levels dependent on infection, with the infected individuals showing greater antibody levels compared to the uninfected individuals (Figure 2).


CD16 expression on monocytes in healthy individuals but not schistosome-infected patients is positively associated with levels of parasite-specific IgG and IgG1.

Appleby LJ, Nausch N, Erskine L, Bourke CD, Rujeni N, Midzi N, Mduluza T, Mutapi F - PLoS Negl Trop Dis (2014)

Total adult worm-specific IgG levels differ by age group in healthy participants but not schistosome patients.Adult worm specific (A) total IgG, (B) IgG1, (C) IgG2, (D) IgG3, (E) IgG4 responses by age group and infection status measured by ELISA. Open circles and dashed lines, healthy individuals; closed circles and solid lines, schistosome patients. Bars represent standard error of mean. Significant differences between schistosome infected and uninfected participants within age groups according to sub-group analysis are indicated by p: ** p≤0.005, *p≤0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125298&req=5

pntd-0003049-g002: Total adult worm-specific IgG levels differ by age group in healthy participants but not schistosome patients.Adult worm specific (A) total IgG, (B) IgG1, (C) IgG2, (D) IgG3, (E) IgG4 responses by age group and infection status measured by ELISA. Open circles and dashed lines, healthy individuals; closed circles and solid lines, schistosome patients. Bars represent standard error of mean. Significant differences between schistosome infected and uninfected participants within age groups according to sub-group analysis are indicated by p: ** p≤0.005, *p≤0.05.
Mentions: The relationship between SWAP-specific total IgG, as well as the IgG subclasses and infection status dependent on age was investigated. Total IgG, IgG1, IgG2 and IgG4, but not IgG3, significantly varied with host age, with total schistosome-specific IgG levels lowest in the age of peak schistosome infection, but the adult worm-specific subclasses IgG1, IgG2 and IgG4 highest in the oldest age group (Table 3). Overall, levels of parasite-specific IgG and IgG1 varied between healthy participants compared to schistosome infected people, with infected individuals having greater antibody levels compared to uninfected individuals (Table 3). However, only for total IgG, the effects of infection status varied with host age as indicated by the significant age group-infection status interaction term (Table 3). Figure 2 demonstrates the gradual increase in levels of schistosome-specific total IgG in healthy participants, while in schistosome infected patients, total IgG levels did not change significantly with age. For total IgG, IgG1, IgG3 and IgG4, but not IgG2, the youngest age group showed significant differences in antibody levels dependent on infection, with the infected individuals showing greater antibody levels compared to the uninfected individuals (Figure 2).

Bottom Line: Human IgG1 antibody responses are associated with protection against Schistosoma haematobium infection and are now a target for schistosome vaccine development.Systemic levels of schistosome-specific anti-adult worm total IgG and IgG subclass titres were measured by ELISA in 100 individuals from an S. haematobium endemic area in Zimbabwe and, using parametric statistical methods and regression analysis, related to the levels of CD16 expression on individuals' circulating monocytes, determined via flow cytometry.Further understanding the elements of a protective immune response in schistosomiasis may aid in efforts to develop a protective vaccine against this disease.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology & Infection Research, Centre for Immunity, Infection & Evolution, School of Biological Sciences, University of Edinburgh, United Kingdom.

ABSTRACT
Human IgG1 antibody responses are associated with protection against Schistosoma haematobium infection and are now a target for schistosome vaccine development. This study aimed to investigate the relationship between total IgG and the IgG subclasses and the monocyte IgG receptor, known as FcγRIIIa or CD16, in schistosome exposed people. Systemic levels of schistosome-specific anti-adult worm total IgG and IgG subclass titres were measured by ELISA in 100 individuals from an S. haematobium endemic area in Zimbabwe and, using parametric statistical methods and regression analysis, related to the levels of CD16 expression on individuals' circulating monocytes, determined via flow cytometry. Monocyte CD16 expression rose with parasite-specific total IgG and IgG1 in healthy participants, but not in schistosome infected patients. Similar to parasite-specific IgG and IgG1, CD16 expression in healthy individuals is associated with protection against schistosome infection. This relationship indicates a mechanistic link between the innate and adaptive immune responses to helminth infection in protection against infection. Further understanding the elements of a protective immune response in schistosomiasis may aid in efforts to develop a protective vaccine against this disease.

Show MeSH
Related in: MedlinePlus