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CD16 expression on monocytes in healthy individuals but not schistosome-infected patients is positively associated with levels of parasite-specific IgG and IgG1.

Appleby LJ, Nausch N, Erskine L, Bourke CD, Rujeni N, Midzi N, Mduluza T, Mutapi F - PLoS Negl Trop Dis (2014)

Bottom Line: Human IgG1 antibody responses are associated with protection against Schistosoma haematobium infection and are now a target for schistosome vaccine development.Systemic levels of schistosome-specific anti-adult worm total IgG and IgG subclass titres were measured by ELISA in 100 individuals from an S. haematobium endemic area in Zimbabwe and, using parametric statistical methods and regression analysis, related to the levels of CD16 expression on individuals' circulating monocytes, determined via flow cytometry.Further understanding the elements of a protective immune response in schistosomiasis may aid in efforts to develop a protective vaccine against this disease.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology & Infection Research, Centre for Immunity, Infection & Evolution, School of Biological Sciences, University of Edinburgh, United Kingdom.

ABSTRACT
Human IgG1 antibody responses are associated with protection against Schistosoma haematobium infection and are now a target for schistosome vaccine development. This study aimed to investigate the relationship between total IgG and the IgG subclasses and the monocyte IgG receptor, known as FcγRIIIa or CD16, in schistosome exposed people. Systemic levels of schistosome-specific anti-adult worm total IgG and IgG subclass titres were measured by ELISA in 100 individuals from an S. haematobium endemic area in Zimbabwe and, using parametric statistical methods and regression analysis, related to the levels of CD16 expression on individuals' circulating monocytes, determined via flow cytometry. Monocyte CD16 expression rose with parasite-specific total IgG and IgG1 in healthy participants, but not in schistosome infected patients. Similar to parasite-specific IgG and IgG1, CD16 expression in healthy individuals is associated with protection against schistosome infection. This relationship indicates a mechanistic link between the innate and adaptive immune responses to helminth infection in protection against infection. Further understanding the elements of a protective immune response in schistosomiasis may aid in efforts to develop a protective vaccine against this disease.

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Related in: MedlinePlus

CD16 but not CD14 expression intensity on circulating monocytes differs between uninfected and infected older patients.Proportion of (A) total circulating monocytes, (B) MFI of CD16, and (C) MFI of CD14 on monocytes according to age group and infection status and as determined by flow cytometry. Open bars and open circles with dashed lines, healthy individuals; filled bars and closed circles with solid lines, schistosome infected patients. Bars represent standard error of mean. Significant differences between infected and uninfected participants within age groups according to sub-group analysis are indicated by *(p≤0.05).
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pntd-0003049-g001: CD16 but not CD14 expression intensity on circulating monocytes differs between uninfected and infected older patients.Proportion of (A) total circulating monocytes, (B) MFI of CD16, and (C) MFI of CD14 on monocytes according to age group and infection status and as determined by flow cytometry. Open bars and open circles with dashed lines, healthy individuals; filled bars and closed circles with solid lines, schistosome infected patients. Bars represent standard error of mean. Significant differences between infected and uninfected participants within age groups according to sub-group analysis are indicated by *(p≤0.05).

Mentions: The proportion of monocytes in PBMCs did not vary significantly with host age or infection as shown in Figure 1A. Thus, any changes observed in subsequent analyses will be a result of changes in monocyte phenotype between individuals, rather than as a result of changes in monocyte proportions, for example due to migration of monocytes into or out of the vasculature.


CD16 expression on monocytes in healthy individuals but not schistosome-infected patients is positively associated with levels of parasite-specific IgG and IgG1.

Appleby LJ, Nausch N, Erskine L, Bourke CD, Rujeni N, Midzi N, Mduluza T, Mutapi F - PLoS Negl Trop Dis (2014)

CD16 but not CD14 expression intensity on circulating monocytes differs between uninfected and infected older patients.Proportion of (A) total circulating monocytes, (B) MFI of CD16, and (C) MFI of CD14 on monocytes according to age group and infection status and as determined by flow cytometry. Open bars and open circles with dashed lines, healthy individuals; filled bars and closed circles with solid lines, schistosome infected patients. Bars represent standard error of mean. Significant differences between infected and uninfected participants within age groups according to sub-group analysis are indicated by *(p≤0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125298&req=5

pntd-0003049-g001: CD16 but not CD14 expression intensity on circulating monocytes differs between uninfected and infected older patients.Proportion of (A) total circulating monocytes, (B) MFI of CD16, and (C) MFI of CD14 on monocytes according to age group and infection status and as determined by flow cytometry. Open bars and open circles with dashed lines, healthy individuals; filled bars and closed circles with solid lines, schistosome infected patients. Bars represent standard error of mean. Significant differences between infected and uninfected participants within age groups according to sub-group analysis are indicated by *(p≤0.05).
Mentions: The proportion of monocytes in PBMCs did not vary significantly with host age or infection as shown in Figure 1A. Thus, any changes observed in subsequent analyses will be a result of changes in monocyte phenotype between individuals, rather than as a result of changes in monocyte proportions, for example due to migration of monocytes into or out of the vasculature.

Bottom Line: Human IgG1 antibody responses are associated with protection against Schistosoma haematobium infection and are now a target for schistosome vaccine development.Systemic levels of schistosome-specific anti-adult worm total IgG and IgG subclass titres were measured by ELISA in 100 individuals from an S. haematobium endemic area in Zimbabwe and, using parametric statistical methods and regression analysis, related to the levels of CD16 expression on individuals' circulating monocytes, determined via flow cytometry.Further understanding the elements of a protective immune response in schistosomiasis may aid in efforts to develop a protective vaccine against this disease.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology & Infection Research, Centre for Immunity, Infection & Evolution, School of Biological Sciences, University of Edinburgh, United Kingdom.

ABSTRACT
Human IgG1 antibody responses are associated with protection against Schistosoma haematobium infection and are now a target for schistosome vaccine development. This study aimed to investigate the relationship between total IgG and the IgG subclasses and the monocyte IgG receptor, known as FcγRIIIa or CD16, in schistosome exposed people. Systemic levels of schistosome-specific anti-adult worm total IgG and IgG subclass titres were measured by ELISA in 100 individuals from an S. haematobium endemic area in Zimbabwe and, using parametric statistical methods and regression analysis, related to the levels of CD16 expression on individuals' circulating monocytes, determined via flow cytometry. Monocyte CD16 expression rose with parasite-specific total IgG and IgG1 in healthy participants, but not in schistosome infected patients. Similar to parasite-specific IgG and IgG1, CD16 expression in healthy individuals is associated with protection against schistosome infection. This relationship indicates a mechanistic link between the innate and adaptive immune responses to helminth infection in protection against infection. Further understanding the elements of a protective immune response in schistosomiasis may aid in efforts to develop a protective vaccine against this disease.

Show MeSH
Related in: MedlinePlus