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Heterogeneous feeding patterns of the dengue vector, Aedes aegypti, on individual human hosts in rural Thailand.

Harrington LC, Fleisher A, Ruiz-Moreno D, Vermeylen F, Wa CV, Poulson RL, Edman JD, Clark JM, Jones JW, Kitthawee S, Scott TW - PLoS Negl Trop Dis (2014)

Bottom Line: Interaction networks for mosquitoes and humans revealed biologically significant blood feeding hotspots, including community marketplaces.High multiple-feeding rates and feeding on community visitors are likely important features in the efficient transmission and rapid spread of DENV.These results help explain why reducing vector populations alone is difficult for dengue prevention and support the argument for additional studies of mosquito feeding behavior, which when integrated with a greater understanding of human behavior will refine estimates of risk and strategies for dengue control.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, Cornell University, Ithaca, New York, United States of America.

ABSTRACT

Background: Mosquito biting frequency and how bites are distributed among different people can have significant epidemiologic effects. An improved understanding of mosquito vector-human interactions would refine knowledge of the entomological processes supporting pathogen transmission and could reveal targets for minimizing risk and breaking pathogen transmission cycles.

Methodology and principal findings: We used human DNA blood meal profiling of the dengue virus (DENV) vector, Aedes aegypti, to quantify its contact with human hosts and to infer epidemiologic implications of its blood feeding behavior. We determined the number of different people bitten, biting frequency by host age, size, mosquito age, and the number of times each person was bitten. Of 3,677 engorged mosquitoes collected and 1,186 complete DNA profiles, only 420 meals matched people from the study area, indicating that Ae. aegypti feed on people moving transiently through communities to conduct daily business. 10-13% of engorged mosquitoes fed on more than one person. No biting rate differences were detected between high- and low-dengue transmission seasons. We estimate that 43-46% of engorged mosquitoes bit more than one person within each gonotrophic cycle. Most multiple meals were from residents of the mosquito collection house or neighbors. People ≤ 25 years old were bitten less often than older people. Some hosts were fed on frequently, with three hosts bitten nine times. Interaction networks for mosquitoes and humans revealed biologically significant blood feeding hotspots, including community marketplaces.

Conclusion and significance: High multiple-feeding rates and feeding on community visitors are likely important features in the efficient transmission and rapid spread of DENV. These results help explain why reducing vector populations alone is difficult for dengue prevention and support the argument for additional studies of mosquito feeding behavior, which when integrated with a greater understanding of human behavior will refine estimates of risk and strategies for dengue control.

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Related in: MedlinePlus

Local clustering for house resident meals for Lao Bao (top) and Pai Lom (bottom) during the rainy season of 2002 with neighbors defined at 40 m and significance evaluated using 9,999 randomizations.Red and blue indicate the presence of hot and cold spots, respectively. Triangle indicates the local market. Black lines represent roads.
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pntd-0003048-g005: Local clustering for house resident meals for Lao Bao (top) and Pai Lom (bottom) during the rainy season of 2002 with neighbors defined at 40 m and significance evaluated using 9,999 randomizations.Red and blue indicate the presence of hot and cold spots, respectively. Triangle indicates the local market. Black lines represent roads.

Mentions: Clustering patterns for mosquito biting was associated with the presence of hot and cold spots in the villages (Fig. 5). These demonstrated more (hot) and less (cold) than expected resident and non-resident blood meals, respectively. Hot and cold spots were detected at several scales during most of the sampling events in both Lao Bao and Pai Lom (Table S1.A–D). Values in Table 8 indicated with parenthesis and a star symbol were those that included the village local market. Values report the interval of distances (m) where significant clustering of bites was detected. For example, in Table 8 B for Lao Bao during the 2001 warm rainy season, hot spots were detected with neighboring houses if the distance among them was 30–60 m, 80 m, and 100–130 m. For 60 m, the clustering included the local market. Although statistically significant, reported clusters at scales bigger than 60 for Lao Bao may not be accurate because of statistical constraints due to the size of the village which was smaller than the two larger cluster sizes. For Lao Bao, local clustering for “resident” bites was observed only during the rainy seasons (2001 and 2002), and in both cases hot spots included the local market. Local clustering for “non-resident” bites was detected for all the sampling events, and the local market was included when neighborhoods were calculated at 80 m or less.


Heterogeneous feeding patterns of the dengue vector, Aedes aegypti, on individual human hosts in rural Thailand.

Harrington LC, Fleisher A, Ruiz-Moreno D, Vermeylen F, Wa CV, Poulson RL, Edman JD, Clark JM, Jones JW, Kitthawee S, Scott TW - PLoS Negl Trop Dis (2014)

Local clustering for house resident meals for Lao Bao (top) and Pai Lom (bottom) during the rainy season of 2002 with neighbors defined at 40 m and significance evaluated using 9,999 randomizations.Red and blue indicate the presence of hot and cold spots, respectively. Triangle indicates the local market. Black lines represent roads.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4125296&req=5

pntd-0003048-g005: Local clustering for house resident meals for Lao Bao (top) and Pai Lom (bottom) during the rainy season of 2002 with neighbors defined at 40 m and significance evaluated using 9,999 randomizations.Red and blue indicate the presence of hot and cold spots, respectively. Triangle indicates the local market. Black lines represent roads.
Mentions: Clustering patterns for mosquito biting was associated with the presence of hot and cold spots in the villages (Fig. 5). These demonstrated more (hot) and less (cold) than expected resident and non-resident blood meals, respectively. Hot and cold spots were detected at several scales during most of the sampling events in both Lao Bao and Pai Lom (Table S1.A–D). Values in Table 8 indicated with parenthesis and a star symbol were those that included the village local market. Values report the interval of distances (m) where significant clustering of bites was detected. For example, in Table 8 B for Lao Bao during the 2001 warm rainy season, hot spots were detected with neighboring houses if the distance among them was 30–60 m, 80 m, and 100–130 m. For 60 m, the clustering included the local market. Although statistically significant, reported clusters at scales bigger than 60 for Lao Bao may not be accurate because of statistical constraints due to the size of the village which was smaller than the two larger cluster sizes. For Lao Bao, local clustering for “resident” bites was observed only during the rainy seasons (2001 and 2002), and in both cases hot spots included the local market. Local clustering for “non-resident” bites was detected for all the sampling events, and the local market was included when neighborhoods were calculated at 80 m or less.

Bottom Line: Interaction networks for mosquitoes and humans revealed biologically significant blood feeding hotspots, including community marketplaces.High multiple-feeding rates and feeding on community visitors are likely important features in the efficient transmission and rapid spread of DENV.These results help explain why reducing vector populations alone is difficult for dengue prevention and support the argument for additional studies of mosquito feeding behavior, which when integrated with a greater understanding of human behavior will refine estimates of risk and strategies for dengue control.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, Cornell University, Ithaca, New York, United States of America.

ABSTRACT

Background: Mosquito biting frequency and how bites are distributed among different people can have significant epidemiologic effects. An improved understanding of mosquito vector-human interactions would refine knowledge of the entomological processes supporting pathogen transmission and could reveal targets for minimizing risk and breaking pathogen transmission cycles.

Methodology and principal findings: We used human DNA blood meal profiling of the dengue virus (DENV) vector, Aedes aegypti, to quantify its contact with human hosts and to infer epidemiologic implications of its blood feeding behavior. We determined the number of different people bitten, biting frequency by host age, size, mosquito age, and the number of times each person was bitten. Of 3,677 engorged mosquitoes collected and 1,186 complete DNA profiles, only 420 meals matched people from the study area, indicating that Ae. aegypti feed on people moving transiently through communities to conduct daily business. 10-13% of engorged mosquitoes fed on more than one person. No biting rate differences were detected between high- and low-dengue transmission seasons. We estimate that 43-46% of engorged mosquitoes bit more than one person within each gonotrophic cycle. Most multiple meals were from residents of the mosquito collection house or neighbors. People ≤ 25 years old were bitten less often than older people. Some hosts were fed on frequently, with three hosts bitten nine times. Interaction networks for mosquitoes and humans revealed biologically significant blood feeding hotspots, including community marketplaces.

Conclusion and significance: High multiple-feeding rates and feeding on community visitors are likely important features in the efficient transmission and rapid spread of DENV. These results help explain why reducing vector populations alone is difficult for dengue prevention and support the argument for additional studies of mosquito feeding behavior, which when integrated with a greater understanding of human behavior will refine estimates of risk and strategies for dengue control.

Show MeSH
Related in: MedlinePlus