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Molecular detection of adenoviruses, rhabdoviruses, and paramyxoviruses in bats from Kenya.

Conrardy C, Tao Y, Kuzmin IV, Niezgoda M, Agwanda B, Breiman RF, Anderson LJ, Rupprecht CE, Tong S - Am. J. Trop. Med. Hyg. (2014)

Bottom Line: We screened 217 bats of at least 20 species from 17 locations in Kenya during July and August of 2006 for the presence of adenovirus, rhabdovirus, and paramyxovirus nucleic acids using generic reverse transcription polymerase chain reaction (RT-PCR) and PCR assays.By sequence comparison and phylogenetic analysis, the Kenya bat paramyxoviruses and rhabdoviruses from this study may represent novel viral lineages within their respective families; the Kenya bat adenoviruses could not be confirmed as novel, because the same region sequences from other known bat adenovirus genomes for comparison were lacking.Our study adds to previous evidence that bats carry diverse, potentially zoonotic viruses and may be coinfected with more than one virus.

View Article: PubMed Central - PubMed

Affiliation: Division of Viral Disease, Centers for Disease Control and Prevention, Atlanta, Georgia; National Center for Emerging and Zoonotic and Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; The Global Alliance for Rabies Control, Manhattan, Kansas; Zoology Department, National Museum, Nairobi, Kenya; Centers for Disease Control and Prevention-Kenya, Nairobi, Kenya; Emory University, Atlanta, Georgia.

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Phylogenetic analysis of adenoviruses. The adenovirus phylogenetic tree was generated using the partial hexon gene sequences (630 nucleotides) from a representative sample comprising 47 known adenoviruses. Viral sequences identified in bats from Kenya are noted by black circles. The trees are drawn to scale, with branch lengths measured in the number of substitutions per site. The Bayesian posterior probabilities (> 0.5) are shown at nodes.
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Figure 1: Phylogenetic analysis of adenoviruses. The adenovirus phylogenetic tree was generated using the partial hexon gene sequences (630 nucleotides) from a representative sample comprising 47 known adenoviruses. Viral sequences identified in bats from Kenya are noted by black circles. The trees are drawn to scale, with branch lengths measured in the number of substitutions per site. The Bayesian posterior probabilities (> 0.5) are shown at nodes.

Mentions: Phylogenetic analysis of four Kenya bat adenoviruses based on partial hexon gene sequences (630 base pairs) and representative sequences comprising 47 known adenoviruses (Figure 1) showed that these viruses grouped together within the Mastadenovirus genus and were most closely related to canine adenovirus types 1 and 2 and bat adenoviruses from China (bat adenovirus 3 from Myotis ricketti) and Germany (bat adenovirus 2 from Pipistrellus pipistrellus).11,12 Interestingly, many other bat mastadenoviruses recently identified from China, Hungary, India, Japan, North America, Brazil, and Spain were also shown to be distantly related to canine adenovirus types 1 and 2 based on a different region of DNA polymerase sequences.12–17 These data may suggest that canine adenoviruses 1 and 2 and the bat adenoviruses share a common ancestor. The KY249 and KY339 sequences from Chaerephon sp. bats were nearly identical (99% nucleotide identity) but distinct from KY165 and KY166 sequences obtained from O. martiensseni bats (61–63% nucleotide identity). The KY165 and KY166 sequences shared only 59% nucleotide identity with each other. These four Kenya bat adenovirus sequences share 58–66% nucleotide identity with their most closely related adenoviruses: bat adenoviruses 2 and 3.


Molecular detection of adenoviruses, rhabdoviruses, and paramyxoviruses in bats from Kenya.

Conrardy C, Tao Y, Kuzmin IV, Niezgoda M, Agwanda B, Breiman RF, Anderson LJ, Rupprecht CE, Tong S - Am. J. Trop. Med. Hyg. (2014)

Phylogenetic analysis of adenoviruses. The adenovirus phylogenetic tree was generated using the partial hexon gene sequences (630 nucleotides) from a representative sample comprising 47 known adenoviruses. Viral sequences identified in bats from Kenya are noted by black circles. The trees are drawn to scale, with branch lengths measured in the number of substitutions per site. The Bayesian posterior probabilities (> 0.5) are shown at nodes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125246&req=5

Figure 1: Phylogenetic analysis of adenoviruses. The adenovirus phylogenetic tree was generated using the partial hexon gene sequences (630 nucleotides) from a representative sample comprising 47 known adenoviruses. Viral sequences identified in bats from Kenya are noted by black circles. The trees are drawn to scale, with branch lengths measured in the number of substitutions per site. The Bayesian posterior probabilities (> 0.5) are shown at nodes.
Mentions: Phylogenetic analysis of four Kenya bat adenoviruses based on partial hexon gene sequences (630 base pairs) and representative sequences comprising 47 known adenoviruses (Figure 1) showed that these viruses grouped together within the Mastadenovirus genus and were most closely related to canine adenovirus types 1 and 2 and bat adenoviruses from China (bat adenovirus 3 from Myotis ricketti) and Germany (bat adenovirus 2 from Pipistrellus pipistrellus).11,12 Interestingly, many other bat mastadenoviruses recently identified from China, Hungary, India, Japan, North America, Brazil, and Spain were also shown to be distantly related to canine adenovirus types 1 and 2 based on a different region of DNA polymerase sequences.12–17 These data may suggest that canine adenoviruses 1 and 2 and the bat adenoviruses share a common ancestor. The KY249 and KY339 sequences from Chaerephon sp. bats were nearly identical (99% nucleotide identity) but distinct from KY165 and KY166 sequences obtained from O. martiensseni bats (61–63% nucleotide identity). The KY165 and KY166 sequences shared only 59% nucleotide identity with each other. These four Kenya bat adenovirus sequences share 58–66% nucleotide identity with their most closely related adenoviruses: bat adenoviruses 2 and 3.

Bottom Line: We screened 217 bats of at least 20 species from 17 locations in Kenya during July and August of 2006 for the presence of adenovirus, rhabdovirus, and paramyxovirus nucleic acids using generic reverse transcription polymerase chain reaction (RT-PCR) and PCR assays.By sequence comparison and phylogenetic analysis, the Kenya bat paramyxoviruses and rhabdoviruses from this study may represent novel viral lineages within their respective families; the Kenya bat adenoviruses could not be confirmed as novel, because the same region sequences from other known bat adenovirus genomes for comparison were lacking.Our study adds to previous evidence that bats carry diverse, potentially zoonotic viruses and may be coinfected with more than one virus.

View Article: PubMed Central - PubMed

Affiliation: Division of Viral Disease, Centers for Disease Control and Prevention, Atlanta, Georgia; National Center for Emerging and Zoonotic and Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; The Global Alliance for Rabies Control, Manhattan, Kansas; Zoology Department, National Museum, Nairobi, Kenya; Centers for Disease Control and Prevention-Kenya, Nairobi, Kenya; Emory University, Atlanta, Georgia.

Show MeSH
Related in: MedlinePlus