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Icariin ameliorates neuropathological changes, TGF-β1 accumulation and behavioral deficits in a mouse model of cerebral amyloidosis.

Zhang ZY, Li C, Zug C, Schluesener HJ - PLoS ONE (2014)

Bottom Line: Icariin, a major constituent of flavonoids from the Chinese medicinal herb Epimedium brevicornum, exhibits multiple biological properties, including anti-inflammatory, neuroregulatory and neuroprotective activities.Therapeutic effects were monitored by behavioral tests, namely nesting assay, before and during the experimental treatment.Our results suggest that Icariin might be considered a promising therapeutic option for human AD.

View Article: PubMed Central - PubMed

Affiliation: Division of Immunopathology of the Nervous System, Institute of Pathology and Neuropathology, University of Tuebingen, Tuebingen, Germany.

ABSTRACT
Icariin, a major constituent of flavonoids from the Chinese medicinal herb Epimedium brevicornum, exhibits multiple biological properties, including anti-inflammatory, neuroregulatory and neuroprotective activities. Therefore, Icariin might be applied in treatment of neurodegenerative disorders, including Alzheimer's disease (AD), which is neuropathologically characterized by β-amyloid aggregation, hyperphosphorylated tau and neuroinflammation. Potential therapeutic effects of Icariin were investigated in an animal model of cerebral amyloidosis for AD, transgenic APP/PS1 mouse. Icariin was suspended in carboxymethylcellulose and given orally to APP/PS1 mice. Therapeutic effects were monitored by behavioral tests, namely nesting assay, before and during the experimental treatment. Following an oral treatment of 10 days, Icariin significantly attenuated Aβ deposition, microglial activation and TGF-β1 immunoreactivity at amyloid plaques in cortex and hippocampus of transgenic mice 5 months of age, and restored impaired nesting ability. Our results suggest that Icariin might be considered a promising therapeutic option for human AD.

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Related in: MedlinePlus

Therapeutic effect of Icariin on Aβ deposition and microglial activation.Representative microimages show the changes in Aβ deposition and microglial activation in cortex and hippocampus following Icariin treatment. A–B and E–F: In both cortex (A–B) and hippocampus (E–F) of APP/PS1 mice from the Icariin group (B and F), reduced numbers of Aβ plaques with relatively smaller size were observed, compared to the control group (A and E). C–D (cortex) and G–H (hippocampus): According to serial sections of Aβ staining, most Iba-1+ microglia accumulated at or surrounding Aβ plaques. In the treatment group (D and H) fewer numbers of Iba-1+ cells and smaller IR area of Iba-1 could be seen, compared to the control group (C and G).
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pone-0104616-g003: Therapeutic effect of Icariin on Aβ deposition and microglial activation.Representative microimages show the changes in Aβ deposition and microglial activation in cortex and hippocampus following Icariin treatment. A–B and E–F: In both cortex (A–B) and hippocampus (E–F) of APP/PS1 mice from the Icariin group (B and F), reduced numbers of Aβ plaques with relatively smaller size were observed, compared to the control group (A and E). C–D (cortex) and G–H (hippocampus): According to serial sections of Aβ staining, most Iba-1+ microglia accumulated at or surrounding Aβ plaques. In the treatment group (D and H) fewer numbers of Iba-1+ cells and smaller IR area of Iba-1 could be seen, compared to the control group (C and G).

Mentions: In brain of APP/PS1 transgenic mice from the control group, Aβ plaques were distributed throughout the whole cortex. Plaques were of different sizes, most small plaques had dense cores and larger plaques mainly consisted of a dense core surrounded by a large halo of diffuse amyloid (Figure 3A). In the hippocampus, plaque density was lower and most plaques were of smaller size (Figure 3E).


Icariin ameliorates neuropathological changes, TGF-β1 accumulation and behavioral deficits in a mouse model of cerebral amyloidosis.

Zhang ZY, Li C, Zug C, Schluesener HJ - PLoS ONE (2014)

Therapeutic effect of Icariin on Aβ deposition and microglial activation.Representative microimages show the changes in Aβ deposition and microglial activation in cortex and hippocampus following Icariin treatment. A–B and E–F: In both cortex (A–B) and hippocampus (E–F) of APP/PS1 mice from the Icariin group (B and F), reduced numbers of Aβ plaques with relatively smaller size were observed, compared to the control group (A and E). C–D (cortex) and G–H (hippocampus): According to serial sections of Aβ staining, most Iba-1+ microglia accumulated at or surrounding Aβ plaques. In the treatment group (D and H) fewer numbers of Iba-1+ cells and smaller IR area of Iba-1 could be seen, compared to the control group (C and G).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4125230&req=5

pone-0104616-g003: Therapeutic effect of Icariin on Aβ deposition and microglial activation.Representative microimages show the changes in Aβ deposition and microglial activation in cortex and hippocampus following Icariin treatment. A–B and E–F: In both cortex (A–B) and hippocampus (E–F) of APP/PS1 mice from the Icariin group (B and F), reduced numbers of Aβ plaques with relatively smaller size were observed, compared to the control group (A and E). C–D (cortex) and G–H (hippocampus): According to serial sections of Aβ staining, most Iba-1+ microglia accumulated at or surrounding Aβ plaques. In the treatment group (D and H) fewer numbers of Iba-1+ cells and smaller IR area of Iba-1 could be seen, compared to the control group (C and G).
Mentions: In brain of APP/PS1 transgenic mice from the control group, Aβ plaques were distributed throughout the whole cortex. Plaques were of different sizes, most small plaques had dense cores and larger plaques mainly consisted of a dense core surrounded by a large halo of diffuse amyloid (Figure 3A). In the hippocampus, plaque density was lower and most plaques were of smaller size (Figure 3E).

Bottom Line: Icariin, a major constituent of flavonoids from the Chinese medicinal herb Epimedium brevicornum, exhibits multiple biological properties, including anti-inflammatory, neuroregulatory and neuroprotective activities.Therapeutic effects were monitored by behavioral tests, namely nesting assay, before and during the experimental treatment.Our results suggest that Icariin might be considered a promising therapeutic option for human AD.

View Article: PubMed Central - PubMed

Affiliation: Division of Immunopathology of the Nervous System, Institute of Pathology and Neuropathology, University of Tuebingen, Tuebingen, Germany.

ABSTRACT
Icariin, a major constituent of flavonoids from the Chinese medicinal herb Epimedium brevicornum, exhibits multiple biological properties, including anti-inflammatory, neuroregulatory and neuroprotective activities. Therefore, Icariin might be applied in treatment of neurodegenerative disorders, including Alzheimer's disease (AD), which is neuropathologically characterized by β-amyloid aggregation, hyperphosphorylated tau and neuroinflammation. Potential therapeutic effects of Icariin were investigated in an animal model of cerebral amyloidosis for AD, transgenic APP/PS1 mouse. Icariin was suspended in carboxymethylcellulose and given orally to APP/PS1 mice. Therapeutic effects were monitored by behavioral tests, namely nesting assay, before and during the experimental treatment. Following an oral treatment of 10 days, Icariin significantly attenuated Aβ deposition, microglial activation and TGF-β1 immunoreactivity at amyloid plaques in cortex and hippocampus of transgenic mice 5 months of age, and restored impaired nesting ability. Our results suggest that Icariin might be considered a promising therapeutic option for human AD.

Show MeSH
Related in: MedlinePlus