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A novel promising strain of Trichoderma evansii (WF-3) for extracellular α-galactosidase production by utilizing different carbon sources under optimized culture conditions.

Chauhan A, Siddiqi NJ, Sharma B - Biomed Res Int (2014)

Bottom Line: The incubation time, temperature, and pH for the maximum enzyme synthesis were found to be 120 h (5 days), 28°C, and 4.5-5.5, respectively.Among the metal ions tested, Hg was found to be the strongest inhibitor of the enzyme.Among the chelators, EDTA acted as stronger inhibitor than succinic acid.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Science, University of Allahabad, Allahabad 211002, India.

ABSTRACT
A potential fungal strain of Trichoderma sp. (WF-3) was isolated and selected for the production of α-galactosidase. Optimum conditions for mycelial growth and enzyme induction were determined. Basal media selected for the growth of fungal isolate containing different carbon sources like guar gum (GG), soya bean meal (SM), and wheat straw (WS) and combinations of these carbon substrates with basic sugars like galactose and sucrose were used to monitor their effects on α-galactosidase production. The results of this study indicated that galactose and sucrose enhanced the enzyme activity in guar gum (GG) and wheat straw (WS). Maximum α-galactosidase production (213.63 U mL(-1)) was obtained when the basic medium containing GG is supplemented with galactose (5 mg/mL). However, the presence of galactose and sucrose alone in the growth media shows no effect. Soya meal alone was able to support T. evansii to produce maximum enzyme activity (170.36 U mL(-1)). The incubation time, temperature, and pH for the maximum enzyme synthesis were found to be 120 h (5 days), 28°C, and 4.5-5.5, respectively. All the carbon sources tested exhibited maximum enzyme production at 10 mg/mL concentration. Among the metal ions tested, Hg was found to be the strongest inhibitor of the enzyme. Among the chelators, EDTA acted as stronger inhibitor than succinic acid.

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Effect of varying concentrations of different substrate combinations on the excretion of α-Gal activity by T. evansii (WF-3) in the culture medium incubated for fifth and sixth days. GG + SUC = guar gum + sucrose, SM + SUC = soya bean meal + sucrose, WS + SUC = wheat straw + sucrose, and SM = soya meal. The culture conditions were the same as described in Section 2. The experiments have been conducted three times and the values are shown as mean ± SD.
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fig7: Effect of varying concentrations of different substrate combinations on the excretion of α-Gal activity by T. evansii (WF-3) in the culture medium incubated for fifth and sixth days. GG + SUC = guar gum + sucrose, SM + SUC = soya bean meal + sucrose, WS + SUC = wheat straw + sucrose, and SM = soya meal. The culture conditions were the same as described in Section 2. The experiments have been conducted three times and the values are shown as mean ± SD.

Mentions: In present study, three different substrate concentrations, that is, 6, 10, and 14 mg/mL, were used to evaluate the optimum substrate utilization in different standardized culture filtrates, that is, GG + GAL, SM, and WS + GAL, for maximum enzyme production in media. The results from present study indicated that 10 mg/mL of substrate concentration was maximally utilised for optimum enzyme production in media on the fifth day of culture incubation at 28°C, where SM has shown maximum enzyme recovery, that is, 213.02 U/mL, followed by (GG + GAL) (154.02 U/mL) and then with (WS) (148.86 U/mL), while (14 mg/mL) shows decreasing trend, that is, (161.77 U/mL) in (SM), (87.14 U/mL) followed by (79.36 U/mL) in (WS) media and the diluted culture media that is, (6 mg/mL) substrate concentration showed much reduced activity profile as presented in (Figure 7). Some workers demonstrated that considerable increase in reaction efficiency can be achieved by operating at higher GG concentrations than those used in most previous studies (<1%) [52, 53]. Furthermore, those reaction systems such as semisolid particulates can be utilized, with consequent improvements in process ability and decreases in reaction volume compared with dilute viscous solutions. One inference which can be drawn from the present study, however, is that the reduction in reaction efficiency at very high (>40% w/w) GG concentration is not due to irreversible enzyme inactivation [54].


A novel promising strain of Trichoderma evansii (WF-3) for extracellular α-galactosidase production by utilizing different carbon sources under optimized culture conditions.

Chauhan A, Siddiqi NJ, Sharma B - Biomed Res Int (2014)

Effect of varying concentrations of different substrate combinations on the excretion of α-Gal activity by T. evansii (WF-3) in the culture medium incubated for fifth and sixth days. GG + SUC = guar gum + sucrose, SM + SUC = soya bean meal + sucrose, WS + SUC = wheat straw + sucrose, and SM = soya meal. The culture conditions were the same as described in Section 2. The experiments have been conducted three times and the values are shown as mean ± SD.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4121999&req=5

fig7: Effect of varying concentrations of different substrate combinations on the excretion of α-Gal activity by T. evansii (WF-3) in the culture medium incubated for fifth and sixth days. GG + SUC = guar gum + sucrose, SM + SUC = soya bean meal + sucrose, WS + SUC = wheat straw + sucrose, and SM = soya meal. The culture conditions were the same as described in Section 2. The experiments have been conducted three times and the values are shown as mean ± SD.
Mentions: In present study, three different substrate concentrations, that is, 6, 10, and 14 mg/mL, were used to evaluate the optimum substrate utilization in different standardized culture filtrates, that is, GG + GAL, SM, and WS + GAL, for maximum enzyme production in media. The results from present study indicated that 10 mg/mL of substrate concentration was maximally utilised for optimum enzyme production in media on the fifth day of culture incubation at 28°C, where SM has shown maximum enzyme recovery, that is, 213.02 U/mL, followed by (GG + GAL) (154.02 U/mL) and then with (WS) (148.86 U/mL), while (14 mg/mL) shows decreasing trend, that is, (161.77 U/mL) in (SM), (87.14 U/mL) followed by (79.36 U/mL) in (WS) media and the diluted culture media that is, (6 mg/mL) substrate concentration showed much reduced activity profile as presented in (Figure 7). Some workers demonstrated that considerable increase in reaction efficiency can be achieved by operating at higher GG concentrations than those used in most previous studies (<1%) [52, 53]. Furthermore, those reaction systems such as semisolid particulates can be utilized, with consequent improvements in process ability and decreases in reaction volume compared with dilute viscous solutions. One inference which can be drawn from the present study, however, is that the reduction in reaction efficiency at very high (>40% w/w) GG concentration is not due to irreversible enzyme inactivation [54].

Bottom Line: The incubation time, temperature, and pH for the maximum enzyme synthesis were found to be 120 h (5 days), 28°C, and 4.5-5.5, respectively.Among the metal ions tested, Hg was found to be the strongest inhibitor of the enzyme.Among the chelators, EDTA acted as stronger inhibitor than succinic acid.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Science, University of Allahabad, Allahabad 211002, India.

ABSTRACT
A potential fungal strain of Trichoderma sp. (WF-3) was isolated and selected for the production of α-galactosidase. Optimum conditions for mycelial growth and enzyme induction were determined. Basal media selected for the growth of fungal isolate containing different carbon sources like guar gum (GG), soya bean meal (SM), and wheat straw (WS) and combinations of these carbon substrates with basic sugars like galactose and sucrose were used to monitor their effects on α-galactosidase production. The results of this study indicated that galactose and sucrose enhanced the enzyme activity in guar gum (GG) and wheat straw (WS). Maximum α-galactosidase production (213.63 U mL(-1)) was obtained when the basic medium containing GG is supplemented with galactose (5 mg/mL). However, the presence of galactose and sucrose alone in the growth media shows no effect. Soya meal alone was able to support T. evansii to produce maximum enzyme activity (170.36 U mL(-1)). The incubation time, temperature, and pH for the maximum enzyme synthesis were found to be 120 h (5 days), 28°C, and 4.5-5.5, respectively. All the carbon sources tested exhibited maximum enzyme production at 10 mg/mL concentration. Among the metal ions tested, Hg was found to be the strongest inhibitor of the enzyme. Among the chelators, EDTA acted as stronger inhibitor than succinic acid.

Show MeSH
Related in: MedlinePlus