Limits...
A novel promising strain of Trichoderma evansii (WF-3) for extracellular α-galactosidase production by utilizing different carbon sources under optimized culture conditions.

Chauhan A, Siddiqi NJ, Sharma B - Biomed Res Int (2014)

Bottom Line: The incubation time, temperature, and pH for the maximum enzyme synthesis were found to be 120 h (5 days), 28°C, and 4.5-5.5, respectively.Among the metal ions tested, Hg was found to be the strongest inhibitor of the enzyme.Among the chelators, EDTA acted as stronger inhibitor than succinic acid.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Science, University of Allahabad, Allahabad 211002, India.

ABSTRACT
A potential fungal strain of Trichoderma sp. (WF-3) was isolated and selected for the production of α-galactosidase. Optimum conditions for mycelial growth and enzyme induction were determined. Basal media selected for the growth of fungal isolate containing different carbon sources like guar gum (GG), soya bean meal (SM), and wheat straw (WS) and combinations of these carbon substrates with basic sugars like galactose and sucrose were used to monitor their effects on α-galactosidase production. The results of this study indicated that galactose and sucrose enhanced the enzyme activity in guar gum (GG) and wheat straw (WS). Maximum α-galactosidase production (213.63 U mL(-1)) was obtained when the basic medium containing GG is supplemented with galactose (5 mg/mL). However, the presence of galactose and sucrose alone in the growth media shows no effect. Soya meal alone was able to support T. evansii to produce maximum enzyme activity (170.36 U mL(-1)). The incubation time, temperature, and pH for the maximum enzyme synthesis were found to be 120 h (5 days), 28°C, and 4.5-5.5, respectively. All the carbon sources tested exhibited maximum enzyme production at 10 mg/mL concentration. Among the metal ions tested, Hg was found to be the strongest inhibitor of the enzyme. Among the chelators, EDTA acted as stronger inhibitor than succinic acid.

Show MeSH

Related in: MedlinePlus

Effect of incubation time on the production of α-Gal by T. evansii in presence of different combinations of carbon sources. GG + SUC = guar gum + sucrose, SM + SUC = soya bean meal + sucrose, WS + SUC = wheat straw + sucrose, and SUC = sucrose. The experiments have been conducted three times and the values are shown as mean ± SD. Blank bar = not detected (for first day enzyme activity).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4121999&req=5

fig4: Effect of incubation time on the production of α-Gal by T. evansii in presence of different combinations of carbon sources. GG + SUC = guar gum + sucrose, SM + SUC = soya bean meal + sucrose, WS + SUC = wheat straw + sucrose, and SUC = sucrose. The experiments have been conducted three times and the values are shown as mean ± SD. Blank bar = not detected (for first day enzyme activity).

Mentions: While knowing the fact that the monosaccharide requirement for α-Gal induction was not only specific for D-galactose, it can be applied for other sugars as well. In another similar experiment, when SM, GG, and WS were combined with sucrose (Suc) in basal culture media, T. evansii displayed maximum secretion of enzyme on the 5th day, the extent of secretion being the highest in presence of SM + Suc followed by GG + Suc and WS + Suc (Figure 4). Usually, only the substrates of an enzyme or its structural analogs are able to act as inducers. Dey and Pridham [49] showed that the rate of hydrolysis of a substrate or its binding with α-Gal depends upon the substrate having a pyranose ring structure and that the configuration of carbon atoms 1–4 must be similar to that in D-galactose [1]. Addition of sucrose in present study, however, showed significantly lower enzyme activity, the values being 42.26 U/mL, 110.88 U/mL, and 42.66 U/mL when added with GG, SM, and WS, respectively, as compared to the carbon sources alone in media (Figure 2). Surprisingly, in this investigation, sucrose and galactose, when added alone in culture media as carbon sources, sustained substantial growth, but galactose was almost as poor inducer as lactose [45]. This could be due to the presence of invertases, which hydrolyse the sucrose and produce simple sugars in combination with background α-Gal. These sugars could then be used for the production of mycelia mass but were unable for any further inducing α-Gal production.


A novel promising strain of Trichoderma evansii (WF-3) for extracellular α-galactosidase production by utilizing different carbon sources under optimized culture conditions.

Chauhan A, Siddiqi NJ, Sharma B - Biomed Res Int (2014)

Effect of incubation time on the production of α-Gal by T. evansii in presence of different combinations of carbon sources. GG + SUC = guar gum + sucrose, SM + SUC = soya bean meal + sucrose, WS + SUC = wheat straw + sucrose, and SUC = sucrose. The experiments have been conducted three times and the values are shown as mean ± SD. Blank bar = not detected (for first day enzyme activity).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4121999&req=5

fig4: Effect of incubation time on the production of α-Gal by T. evansii in presence of different combinations of carbon sources. GG + SUC = guar gum + sucrose, SM + SUC = soya bean meal + sucrose, WS + SUC = wheat straw + sucrose, and SUC = sucrose. The experiments have been conducted three times and the values are shown as mean ± SD. Blank bar = not detected (for first day enzyme activity).
Mentions: While knowing the fact that the monosaccharide requirement for α-Gal induction was not only specific for D-galactose, it can be applied for other sugars as well. In another similar experiment, when SM, GG, and WS were combined with sucrose (Suc) in basal culture media, T. evansii displayed maximum secretion of enzyme on the 5th day, the extent of secretion being the highest in presence of SM + Suc followed by GG + Suc and WS + Suc (Figure 4). Usually, only the substrates of an enzyme or its structural analogs are able to act as inducers. Dey and Pridham [49] showed that the rate of hydrolysis of a substrate or its binding with α-Gal depends upon the substrate having a pyranose ring structure and that the configuration of carbon atoms 1–4 must be similar to that in D-galactose [1]. Addition of sucrose in present study, however, showed significantly lower enzyme activity, the values being 42.26 U/mL, 110.88 U/mL, and 42.66 U/mL when added with GG, SM, and WS, respectively, as compared to the carbon sources alone in media (Figure 2). Surprisingly, in this investigation, sucrose and galactose, when added alone in culture media as carbon sources, sustained substantial growth, but galactose was almost as poor inducer as lactose [45]. This could be due to the presence of invertases, which hydrolyse the sucrose and produce simple sugars in combination with background α-Gal. These sugars could then be used for the production of mycelia mass but were unable for any further inducing α-Gal production.

Bottom Line: The incubation time, temperature, and pH for the maximum enzyme synthesis were found to be 120 h (5 days), 28°C, and 4.5-5.5, respectively.Among the metal ions tested, Hg was found to be the strongest inhibitor of the enzyme.Among the chelators, EDTA acted as stronger inhibitor than succinic acid.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Science, University of Allahabad, Allahabad 211002, India.

ABSTRACT
A potential fungal strain of Trichoderma sp. (WF-3) was isolated and selected for the production of α-galactosidase. Optimum conditions for mycelial growth and enzyme induction were determined. Basal media selected for the growth of fungal isolate containing different carbon sources like guar gum (GG), soya bean meal (SM), and wheat straw (WS) and combinations of these carbon substrates with basic sugars like galactose and sucrose were used to monitor their effects on α-galactosidase production. The results of this study indicated that galactose and sucrose enhanced the enzyme activity in guar gum (GG) and wheat straw (WS). Maximum α-galactosidase production (213.63 U mL(-1)) was obtained when the basic medium containing GG is supplemented with galactose (5 mg/mL). However, the presence of galactose and sucrose alone in the growth media shows no effect. Soya meal alone was able to support T. evansii to produce maximum enzyme activity (170.36 U mL(-1)). The incubation time, temperature, and pH for the maximum enzyme synthesis were found to be 120 h (5 days), 28°C, and 4.5-5.5, respectively. All the carbon sources tested exhibited maximum enzyme production at 10 mg/mL concentration. Among the metal ions tested, Hg was found to be the strongest inhibitor of the enzyme. Among the chelators, EDTA acted as stronger inhibitor than succinic acid.

Show MeSH
Related in: MedlinePlus