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A novel promising strain of Trichoderma evansii (WF-3) for extracellular α-galactosidase production by utilizing different carbon sources under optimized culture conditions.

Chauhan A, Siddiqi NJ, Sharma B - Biomed Res Int (2014)

Bottom Line: The incubation time, temperature, and pH for the maximum enzyme synthesis were found to be 120 h (5 days), 28°C, and 4.5-5.5, respectively.Among the metal ions tested, Hg was found to be the strongest inhibitor of the enzyme.Among the chelators, EDTA acted as stronger inhibitor than succinic acid.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Science, University of Allahabad, Allahabad 211002, India.

ABSTRACT
A potential fungal strain of Trichoderma sp. (WF-3) was isolated and selected for the production of α-galactosidase. Optimum conditions for mycelial growth and enzyme induction were determined. Basal media selected for the growth of fungal isolate containing different carbon sources like guar gum (GG), soya bean meal (SM), and wheat straw (WS) and combinations of these carbon substrates with basic sugars like galactose and sucrose were used to monitor their effects on α-galactosidase production. The results of this study indicated that galactose and sucrose enhanced the enzyme activity in guar gum (GG) and wheat straw (WS). Maximum α-galactosidase production (213.63 U mL(-1)) was obtained when the basic medium containing GG is supplemented with galactose (5 mg/mL). However, the presence of galactose and sucrose alone in the growth media shows no effect. Soya meal alone was able to support T. evansii to produce maximum enzyme activity (170.36 U mL(-1)). The incubation time, temperature, and pH for the maximum enzyme synthesis were found to be 120 h (5 days), 28°C, and 4.5-5.5, respectively. All the carbon sources tested exhibited maximum enzyme production at 10 mg/mL concentration. Among the metal ions tested, Hg was found to be the strongest inhibitor of the enzyme. Among the chelators, EDTA acted as stronger inhibitor than succinic acid.

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Related in: MedlinePlus

Growth pattern of T. evansii in the presence of different carbon sources in the culture medium containing potato-dextrose-agar (PDA, solid medium) (a). After 7 days of growth of the fungal culture in broth containing different carbon sources such as soya meal (SM) (b), guar gum (GG) (c), and wheat straw (WS) (d). The corresponding fungal floccules were stained with cotton blue and viewed under compound microscope for their comparison with that grown in PDA.
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fig1: Growth pattern of T. evansii in the presence of different carbon sources in the culture medium containing potato-dextrose-agar (PDA, solid medium) (a). After 7 days of growth of the fungal culture in broth containing different carbon sources such as soya meal (SM) (b), guar gum (GG) (c), and wheat straw (WS) (d). The corresponding fungal floccules were stained with cotton blue and viewed under compound microscope for their comparison with that grown in PDA.

Mentions: Selected fungi, that is, Trichoderma evansii (WF-3), were isolated from rhizospheric soil of Phyllanthus emblica(aamla) collected from the local garden of Sagar, India [33]. They were isolated by direct soil plate method [34] and identified accordingly [35–37]. Pure cultures were maintained on PDA slants. Growth pattern of T. evansii in the presence of different carbon sources in the culture medium containing potato-dextrose-agar (PDA solid medium) and other carbon sources has been shown in Figure 1(a). After 7 days of growth of the fungal culture in broth containing different carbon sources such as soya meal (SM) Figure 1(b), guar gum (GG) Figure 1(c), and wheat straw (WS) Figure 1(d); the concerned enzyme activities are demonstrated. The corresponding fungal floccules were stained with cotton blue and viewed under compound microscope for their comparison with that grown in PDA (Figure 1).


A novel promising strain of Trichoderma evansii (WF-3) for extracellular α-galactosidase production by utilizing different carbon sources under optimized culture conditions.

Chauhan A, Siddiqi NJ, Sharma B - Biomed Res Int (2014)

Growth pattern of T. evansii in the presence of different carbon sources in the culture medium containing potato-dextrose-agar (PDA, solid medium) (a). After 7 days of growth of the fungal culture in broth containing different carbon sources such as soya meal (SM) (b), guar gum (GG) (c), and wheat straw (WS) (d). The corresponding fungal floccules were stained with cotton blue and viewed under compound microscope for their comparison with that grown in PDA.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4121999&req=5

fig1: Growth pattern of T. evansii in the presence of different carbon sources in the culture medium containing potato-dextrose-agar (PDA, solid medium) (a). After 7 days of growth of the fungal culture in broth containing different carbon sources such as soya meal (SM) (b), guar gum (GG) (c), and wheat straw (WS) (d). The corresponding fungal floccules were stained with cotton blue and viewed under compound microscope for their comparison with that grown in PDA.
Mentions: Selected fungi, that is, Trichoderma evansii (WF-3), were isolated from rhizospheric soil of Phyllanthus emblica(aamla) collected from the local garden of Sagar, India [33]. They were isolated by direct soil plate method [34] and identified accordingly [35–37]. Pure cultures were maintained on PDA slants. Growth pattern of T. evansii in the presence of different carbon sources in the culture medium containing potato-dextrose-agar (PDA solid medium) and other carbon sources has been shown in Figure 1(a). After 7 days of growth of the fungal culture in broth containing different carbon sources such as soya meal (SM) Figure 1(b), guar gum (GG) Figure 1(c), and wheat straw (WS) Figure 1(d); the concerned enzyme activities are demonstrated. The corresponding fungal floccules were stained with cotton blue and viewed under compound microscope for their comparison with that grown in PDA (Figure 1).

Bottom Line: The incubation time, temperature, and pH for the maximum enzyme synthesis were found to be 120 h (5 days), 28°C, and 4.5-5.5, respectively.Among the metal ions tested, Hg was found to be the strongest inhibitor of the enzyme.Among the chelators, EDTA acted as stronger inhibitor than succinic acid.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Science, University of Allahabad, Allahabad 211002, India.

ABSTRACT
A potential fungal strain of Trichoderma sp. (WF-3) was isolated and selected for the production of α-galactosidase. Optimum conditions for mycelial growth and enzyme induction were determined. Basal media selected for the growth of fungal isolate containing different carbon sources like guar gum (GG), soya bean meal (SM), and wheat straw (WS) and combinations of these carbon substrates with basic sugars like galactose and sucrose were used to monitor their effects on α-galactosidase production. The results of this study indicated that galactose and sucrose enhanced the enzyme activity in guar gum (GG) and wheat straw (WS). Maximum α-galactosidase production (213.63 U mL(-1)) was obtained when the basic medium containing GG is supplemented with galactose (5 mg/mL). However, the presence of galactose and sucrose alone in the growth media shows no effect. Soya meal alone was able to support T. evansii to produce maximum enzyme activity (170.36 U mL(-1)). The incubation time, temperature, and pH for the maximum enzyme synthesis were found to be 120 h (5 days), 28°C, and 4.5-5.5, respectively. All the carbon sources tested exhibited maximum enzyme production at 10 mg/mL concentration. Among the metal ions tested, Hg was found to be the strongest inhibitor of the enzyme. Among the chelators, EDTA acted as stronger inhibitor than succinic acid.

Show MeSH
Related in: MedlinePlus