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First record of isolation and characterization of methicillin resistant Staphylococcus lugdunensis from clinical samples in Iraq.

Al-Charrakh AH, Obayes MH - Biomed Res Int (2014)

Bottom Line: The results of oxacillin MIC showed that 7 of the 15 (46.6%) S. lugdunensis isolates were oxacillin resistant.Results of molecular detection of mecA gene found that mecA gene was detected in 6 (40%) of 15 S. lugdunensis.This study is a first record of isolation and characterization of methicillin resistant S. lugdunensis (MRSL) from clinical samples in Iraq.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, College of Medicine, Babylon University, Hilla, Babylon Governorate, Iraq.

ABSTRACT
This study was conducted to determine the frequency of Staphylococcus lugdunensis in different clinical samples. Out of 690 clinical samples, a total of 178 coagulase negative staphylococci (CoNS) isolates were recovered. CoNS were identified as 10 different species; 22 isolates belonged to Staphylococcus lugdunensis. Two specific genes for S. lugdunensis were used (tanA gene and fbl gene) to confirm identification. Both of these specific genes were detected in 15 (68.1%) of 22 isolates that were identified phenotypically. The results of oxacillin MIC showed that 7 of the 15 (46.6%) S. lugdunensis isolates were oxacillin resistant. The antibiotic susceptibility testing against 16 antibiotics showed that resistance rates were variable towards these antibiotics. Eight of fifteen S. lugdunensis isolates (53.3%) were β-lactamase producer. Results of molecular detection of mecA gene found that mecA gene was detected in 6 (40%) of 15 S. lugdunensis. All of these 6 isolates (S1, S2, S3, S4, S5, and S6) were resistant to oxacillin. One isolate (S7) was resistant to oxacillin but mecA was not detected in this isolate. This study is a first record of isolation and characterization of methicillin resistant S. lugdunensis (MRSL) from clinical samples in Iraq.

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Gel electrophoresis of PCR of fbl amplicon (425 bp) product: Lane L: ladder (1000-bp ladder); Lanes S1, and 15: number of S. lugdunensis isolates. C1: S. aureus, C2: S. epidermidis.
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fig3: Gel electrophoresis of PCR of fbl amplicon (425 bp) product: Lane L: ladder (1000-bp ladder); Lanes S1, and 15: number of S. lugdunensis isolates. C1: S. aureus, C2: S. epidermidis.

Mentions: A suitable nucleic acid target to diagnosed S. lugdunensis is fbl gene, which encods a fibrinogen-binding adhesin [15]. The gene was detected in all 15 S. lugdunensis isolates that were positive to tanA in this study (Figure 2), while no amplification product was obtained from S. aureus and S. epidermidis isolates that were used as negative control as in Figure 3.


First record of isolation and characterization of methicillin resistant Staphylococcus lugdunensis from clinical samples in Iraq.

Al-Charrakh AH, Obayes MH - Biomed Res Int (2014)

Gel electrophoresis of PCR of fbl amplicon (425 bp) product: Lane L: ladder (1000-bp ladder); Lanes S1, and 15: number of S. lugdunensis isolates. C1: S. aureus, C2: S. epidermidis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4121997&req=5

fig3: Gel electrophoresis of PCR of fbl amplicon (425 bp) product: Lane L: ladder (1000-bp ladder); Lanes S1, and 15: number of S. lugdunensis isolates. C1: S. aureus, C2: S. epidermidis.
Mentions: A suitable nucleic acid target to diagnosed S. lugdunensis is fbl gene, which encods a fibrinogen-binding adhesin [15]. The gene was detected in all 15 S. lugdunensis isolates that were positive to tanA in this study (Figure 2), while no amplification product was obtained from S. aureus and S. epidermidis isolates that were used as negative control as in Figure 3.

Bottom Line: The results of oxacillin MIC showed that 7 of the 15 (46.6%) S. lugdunensis isolates were oxacillin resistant.Results of molecular detection of mecA gene found that mecA gene was detected in 6 (40%) of 15 S. lugdunensis.This study is a first record of isolation and characterization of methicillin resistant S. lugdunensis (MRSL) from clinical samples in Iraq.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, College of Medicine, Babylon University, Hilla, Babylon Governorate, Iraq.

ABSTRACT
This study was conducted to determine the frequency of Staphylococcus lugdunensis in different clinical samples. Out of 690 clinical samples, a total of 178 coagulase negative staphylococci (CoNS) isolates were recovered. CoNS were identified as 10 different species; 22 isolates belonged to Staphylococcus lugdunensis. Two specific genes for S. lugdunensis were used (tanA gene and fbl gene) to confirm identification. Both of these specific genes were detected in 15 (68.1%) of 22 isolates that were identified phenotypically. The results of oxacillin MIC showed that 7 of the 15 (46.6%) S. lugdunensis isolates were oxacillin resistant. The antibiotic susceptibility testing against 16 antibiotics showed that resistance rates were variable towards these antibiotics. Eight of fifteen S. lugdunensis isolates (53.3%) were β-lactamase producer. Results of molecular detection of mecA gene found that mecA gene was detected in 6 (40%) of 15 S. lugdunensis. All of these 6 isolates (S1, S2, S3, S4, S5, and S6) were resistant to oxacillin. One isolate (S7) was resistant to oxacillin but mecA was not detected in this isolate. This study is a first record of isolation and characterization of methicillin resistant S. lugdunensis (MRSL) from clinical samples in Iraq.

Show MeSH
Related in: MedlinePlus