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Profiling of biomarkers for the exposure of polycyclic aromatic hydrocarbons: lamin-A/C isoform 3, poly[ADP-ribose] polymerase 1, and mitochondria copy number are identified as universal biomarkers.

Kim HY, Kim HR, Kang MG, Trang NT, Baek HJ, Moon JD, Shin JH, Suh SP, Ryang DW, Kook H, Shin MG - Biomed Res Int (2014)

Bottom Line: Among them, lamin-A/C isoform 3 and PARP-1 were further confirmed using mRNA and protein expression study.Obvious morphological abnormalities including curved backbone and cardiomegaly in zebrafish were observed in the 54 hpf with more than 400 nM of benzopyrene.In conclusion, the change of mitochondrial genome (increased mtDNA copy number) was closely associated with PAH exposure in cell lines and mesenchymal stem cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, 160 Ilsimri, Hwasun-eup, Hwasun-gun, Jeollanam-do 519-809, Republic of Korea ; Brain Korea 21 Plus Project, Chonnam National University Medical School, Gwangju, Republic of Korea.

ABSTRACT
This study investigated the profiling of polycyclic aromatic hydrocarbon- (PAH-) induced genotoxicity in cell lines and zebrafish. Each type of cells displayed different proportionality of apoptosis. Mitochondrial DNA (mtDNA) copy number was dramatically elevated after 5-day treatment of fluoranthene and pyrene. The notable deregulated proteins for PAHs exposure were displayed as follows: lamin-A/C isoform 3 and annexin A1 for benzopyrene; lamin-A/C isoform 3 and DNA topoisomerase 2-alpha for pentacene; poly[ADP-ribose] polymerase 1 (PARP-1) for fluoranthene; and talin-1 and DNA topoisomerase 2-alpha for pyrene. Among them, lamin-A/C isoform 3 and PARP-1 were further confirmed using mRNA and protein expression study. Obvious morphological abnormalities including curved backbone and cardiomegaly in zebrafish were observed in the 54 hpf with more than 400 nM of benzopyrene. In conclusion, the change of mitochondrial genome (increased mtDNA copy number) was closely associated with PAH exposure in cell lines and mesenchymal stem cells. Lamin-A/C isoform 3, talin-1, and annexin A1 were identified as universal biomarkers for PAHs exposure. Zebrafish, specifically at embryo stage, showed suitable in vivo model for monitoring PAHs exposure to hematopoietic tissue and other organs.

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Related in: MedlinePlus

Functional grouping of potential candidate biomarkers for PAHs exposure. Identified potential biomarkers were categorized as their biological process (a) and molecular functions (b). These candidate biomarkers for PAHs exposure were isolated using proteomic analysis of mitochondria-rich cellular fraction in THP-1 cell line.
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fig4: Functional grouping of potential candidate biomarkers for PAHs exposure. Identified potential biomarkers were categorized as their biological process (a) and molecular functions (b). These candidate biomarkers for PAHs exposure were isolated using proteomic analysis of mitochondria-rich cellular fraction in THP-1 cell line.

Mentions: Several hundreds of cellular proteins in mitochondrial-rich cytoplasmic fraction were profoundly deregulated in comparison to control group (Figure 4). The notable deregulated proteins for PAHs exposure were displayed as follows: LMNA and annexin A1 for BaP; LMNA and DNA topoisomerase 2-alpha for pentacene; PARP-1 for fluoranthene; and talin-1 and DNA topoisomerase 2-alpha for pyrene (Tables 1 and 2).


Profiling of biomarkers for the exposure of polycyclic aromatic hydrocarbons: lamin-A/C isoform 3, poly[ADP-ribose] polymerase 1, and mitochondria copy number are identified as universal biomarkers.

Kim HY, Kim HR, Kang MG, Trang NT, Baek HJ, Moon JD, Shin JH, Suh SP, Ryang DW, Kook H, Shin MG - Biomed Res Int (2014)

Functional grouping of potential candidate biomarkers for PAHs exposure. Identified potential biomarkers were categorized as their biological process (a) and molecular functions (b). These candidate biomarkers for PAHs exposure were isolated using proteomic analysis of mitochondria-rich cellular fraction in THP-1 cell line.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4121044&req=5

fig4: Functional grouping of potential candidate biomarkers for PAHs exposure. Identified potential biomarkers were categorized as their biological process (a) and molecular functions (b). These candidate biomarkers for PAHs exposure were isolated using proteomic analysis of mitochondria-rich cellular fraction in THP-1 cell line.
Mentions: Several hundreds of cellular proteins in mitochondrial-rich cytoplasmic fraction were profoundly deregulated in comparison to control group (Figure 4). The notable deregulated proteins for PAHs exposure were displayed as follows: LMNA and annexin A1 for BaP; LMNA and DNA topoisomerase 2-alpha for pentacene; PARP-1 for fluoranthene; and talin-1 and DNA topoisomerase 2-alpha for pyrene (Tables 1 and 2).

Bottom Line: Among them, lamin-A/C isoform 3 and PARP-1 were further confirmed using mRNA and protein expression study.Obvious morphological abnormalities including curved backbone and cardiomegaly in zebrafish were observed in the 54 hpf with more than 400 nM of benzopyrene.In conclusion, the change of mitochondrial genome (increased mtDNA copy number) was closely associated with PAH exposure in cell lines and mesenchymal stem cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, 160 Ilsimri, Hwasun-eup, Hwasun-gun, Jeollanam-do 519-809, Republic of Korea ; Brain Korea 21 Plus Project, Chonnam National University Medical School, Gwangju, Republic of Korea.

ABSTRACT
This study investigated the profiling of polycyclic aromatic hydrocarbon- (PAH-) induced genotoxicity in cell lines and zebrafish. Each type of cells displayed different proportionality of apoptosis. Mitochondrial DNA (mtDNA) copy number was dramatically elevated after 5-day treatment of fluoranthene and pyrene. The notable deregulated proteins for PAHs exposure were displayed as follows: lamin-A/C isoform 3 and annexin A1 for benzopyrene; lamin-A/C isoform 3 and DNA topoisomerase 2-alpha for pentacene; poly[ADP-ribose] polymerase 1 (PARP-1) for fluoranthene; and talin-1 and DNA topoisomerase 2-alpha for pyrene. Among them, lamin-A/C isoform 3 and PARP-1 were further confirmed using mRNA and protein expression study. Obvious morphological abnormalities including curved backbone and cardiomegaly in zebrafish were observed in the 54 hpf with more than 400 nM of benzopyrene. In conclusion, the change of mitochondrial genome (increased mtDNA copy number) was closely associated with PAH exposure in cell lines and mesenchymal stem cells. Lamin-A/C isoform 3, talin-1, and annexin A1 were identified as universal biomarkers for PAHs exposure. Zebrafish, specifically at embryo stage, showed suitable in vivo model for monitoring PAHs exposure to hematopoietic tissue and other organs.

Show MeSH
Related in: MedlinePlus