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Profiling of biomarkers for the exposure of polycyclic aromatic hydrocarbons: lamin-A/C isoform 3, poly[ADP-ribose] polymerase 1, and mitochondria copy number are identified as universal biomarkers.

Kim HY, Kim HR, Kang MG, Trang NT, Baek HJ, Moon JD, Shin JH, Suh SP, Ryang DW, Kook H, Shin MG - Biomed Res Int (2014)

Bottom Line: Among them, lamin-A/C isoform 3 and PARP-1 were further confirmed using mRNA and protein expression study.Obvious morphological abnormalities including curved backbone and cardiomegaly in zebrafish were observed in the 54 hpf with more than 400 nM of benzopyrene.In conclusion, the change of mitochondrial genome (increased mtDNA copy number) was closely associated with PAH exposure in cell lines and mesenchymal stem cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, 160 Ilsimri, Hwasun-eup, Hwasun-gun, Jeollanam-do 519-809, Republic of Korea ; Brain Korea 21 Plus Project, Chonnam National University Medical School, Gwangju, Republic of Korea.

ABSTRACT
This study investigated the profiling of polycyclic aromatic hydrocarbon- (PAH-) induced genotoxicity in cell lines and zebrafish. Each type of cells displayed different proportionality of apoptosis. Mitochondrial DNA (mtDNA) copy number was dramatically elevated after 5-day treatment of fluoranthene and pyrene. The notable deregulated proteins for PAHs exposure were displayed as follows: lamin-A/C isoform 3 and annexin A1 for benzopyrene; lamin-A/C isoform 3 and DNA topoisomerase 2-alpha for pentacene; poly[ADP-ribose] polymerase 1 (PARP-1) for fluoranthene; and talin-1 and DNA topoisomerase 2-alpha for pyrene. Among them, lamin-A/C isoform 3 and PARP-1 were further confirmed using mRNA and protein expression study. Obvious morphological abnormalities including curved backbone and cardiomegaly in zebrafish were observed in the 54 hpf with more than 400 nM of benzopyrene. In conclusion, the change of mitochondrial genome (increased mtDNA copy number) was closely associated with PAH exposure in cell lines and mesenchymal stem cells. Lamin-A/C isoform 3, talin-1, and annexin A1 were identified as universal biomarkers for PAHs exposure. Zebrafish, specifically at embryo stage, showed suitable in vivo model for monitoring PAHs exposure to hematopoietic tissue and other organs.

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Related in: MedlinePlus

The change of cell count and viability after PAHs exposure in THP-1 and Molt-4 cell line. Depending on the type of PAHs, each cell count showed different aspects. In comparison to DMSO treated (0.1%) group, fluoranthene displayed profound significant reduction in cell count, especially in THP-1 and Molt-4 cell line ((a) and (b)). Viability was significantly decreased after fluoranthene exposure for two days. On the third day of PAHs exposure, viability was reduced remarkably in both cell lines ((c) and (d)).
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fig2: The change of cell count and viability after PAHs exposure in THP-1 and Molt-4 cell line. Depending on the type of PAHs, each cell count showed different aspects. In comparison to DMSO treated (0.1%) group, fluoranthene displayed profound significant reduction in cell count, especially in THP-1 and Molt-4 cell line ((a) and (b)). Viability was significantly decreased after fluoranthene exposure for two days. On the third day of PAHs exposure, viability was reduced remarkably in both cell lines ((c) and (d)).

Mentions: Depending on the type of PAHs, each cell count showed different aspects. The total number of cells in the THP-1 and Molt-4 cell lines decreased 11 days after PAHs exposure. The change in the total number of cells in the THP-1 and Molt-4 cell lines decreased in a time-dependent manner. In comparison to control group, fluoranthene displayed profound significant reduction in cell count (Figures 2(a) and 2(b)). The change in the total cell count for the THP-1 and Molt-4 cell lines had a similar pattern after PAHs exposure. Cytotoxicity study carried out the experiment with 100 μM of PAHs after selecting the minimum concentration that is poisonous to cells.


Profiling of biomarkers for the exposure of polycyclic aromatic hydrocarbons: lamin-A/C isoform 3, poly[ADP-ribose] polymerase 1, and mitochondria copy number are identified as universal biomarkers.

Kim HY, Kim HR, Kang MG, Trang NT, Baek HJ, Moon JD, Shin JH, Suh SP, Ryang DW, Kook H, Shin MG - Biomed Res Int (2014)

The change of cell count and viability after PAHs exposure in THP-1 and Molt-4 cell line. Depending on the type of PAHs, each cell count showed different aspects. In comparison to DMSO treated (0.1%) group, fluoranthene displayed profound significant reduction in cell count, especially in THP-1 and Molt-4 cell line ((a) and (b)). Viability was significantly decreased after fluoranthene exposure for two days. On the third day of PAHs exposure, viability was reduced remarkably in both cell lines ((c) and (d)).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4121044&req=5

fig2: The change of cell count and viability after PAHs exposure in THP-1 and Molt-4 cell line. Depending on the type of PAHs, each cell count showed different aspects. In comparison to DMSO treated (0.1%) group, fluoranthene displayed profound significant reduction in cell count, especially in THP-1 and Molt-4 cell line ((a) and (b)). Viability was significantly decreased after fluoranthene exposure for two days. On the third day of PAHs exposure, viability was reduced remarkably in both cell lines ((c) and (d)).
Mentions: Depending on the type of PAHs, each cell count showed different aspects. The total number of cells in the THP-1 and Molt-4 cell lines decreased 11 days after PAHs exposure. The change in the total number of cells in the THP-1 and Molt-4 cell lines decreased in a time-dependent manner. In comparison to control group, fluoranthene displayed profound significant reduction in cell count (Figures 2(a) and 2(b)). The change in the total cell count for the THP-1 and Molt-4 cell lines had a similar pattern after PAHs exposure. Cytotoxicity study carried out the experiment with 100 μM of PAHs after selecting the minimum concentration that is poisonous to cells.

Bottom Line: Among them, lamin-A/C isoform 3 and PARP-1 were further confirmed using mRNA and protein expression study.Obvious morphological abnormalities including curved backbone and cardiomegaly in zebrafish were observed in the 54 hpf with more than 400 nM of benzopyrene.In conclusion, the change of mitochondrial genome (increased mtDNA copy number) was closely associated with PAH exposure in cell lines and mesenchymal stem cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, 160 Ilsimri, Hwasun-eup, Hwasun-gun, Jeollanam-do 519-809, Republic of Korea ; Brain Korea 21 Plus Project, Chonnam National University Medical School, Gwangju, Republic of Korea.

ABSTRACT
This study investigated the profiling of polycyclic aromatic hydrocarbon- (PAH-) induced genotoxicity in cell lines and zebrafish. Each type of cells displayed different proportionality of apoptosis. Mitochondrial DNA (mtDNA) copy number was dramatically elevated after 5-day treatment of fluoranthene and pyrene. The notable deregulated proteins for PAHs exposure were displayed as follows: lamin-A/C isoform 3 and annexin A1 for benzopyrene; lamin-A/C isoform 3 and DNA topoisomerase 2-alpha for pentacene; poly[ADP-ribose] polymerase 1 (PARP-1) for fluoranthene; and talin-1 and DNA topoisomerase 2-alpha for pyrene. Among them, lamin-A/C isoform 3 and PARP-1 were further confirmed using mRNA and protein expression study. Obvious morphological abnormalities including curved backbone and cardiomegaly in zebrafish were observed in the 54 hpf with more than 400 nM of benzopyrene. In conclusion, the change of mitochondrial genome (increased mtDNA copy number) was closely associated with PAH exposure in cell lines and mesenchymal stem cells. Lamin-A/C isoform 3, talin-1, and annexin A1 were identified as universal biomarkers for PAHs exposure. Zebrafish, specifically at embryo stage, showed suitable in vivo model for monitoring PAHs exposure to hematopoietic tissue and other organs.

Show MeSH
Related in: MedlinePlus