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Effects of inactivated Bordetella pertussis on phosphodiesterase in the lung of ovalbumin sensitized and challenged rats.

Wang YJ, Song SD, Chen JC, Wang XF, Jiang YL, Xie QM, Chen JQ, Li ZG, Tang HF - Pulm Med (2014)

Bottom Line: This paper indicated that inactivated Bordetella pertussis (iBp) can enhance the lung airway hyperreactivity of the rats sensitized and challenged with OVA.The mechanisms were involved in the upregulation of cAMP-PDE activity and PDE4A, PDE4D, and PDE3 gene expression in the lungs.So, our data suggested that cosensitization with OVA and iBp affects lung airway reactivity by modulating the lung cAMP-PDE activity and PDE4D gene expression.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Respiratory Drugs Research Laboratory of SFDA of China, School of Medicine, Zhejiang University, Hangzhou 310058, China ; Clinical College of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Hefei 230038, China.

ABSTRACT
This paper indicated that inactivated Bordetella pertussis (iBp) can enhance the lung airway hyperreactivity of the rats sensitized and challenged with OVA. The mechanisms were involved in the upregulation of cAMP-PDE activity and PDE4A, PDE4D, and PDE3 gene expression in the lungs. But only PDE4 activity was different between the OVA and OVA+iBp groups, and PDE4D expression was significantly increased in iBp rats alone. So, our data suggested that cosensitization with OVA and iBp affects lung airway reactivity by modulating the lung cAMP-PDE activity and PDE4D gene expression.

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Related in: MedlinePlus

(a) Representative image of PDE subtype mRNA expression. (b) Levels of mRNA expression of lung PDE subtypes in normal (n = 8), OVA+iBp, iBp (n = 6), and OVA rats (n = 8). Data are expressed relative to β-actin (*P < 0.05; **P < 0.01 versus OVA+iBp; #P < 0.05; ##P < 0.01 versus normal).
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fig5: (a) Representative image of PDE subtype mRNA expression. (b) Levels of mRNA expression of lung PDE subtypes in normal (n = 8), OVA+iBp, iBp (n = 6), and OVA rats (n = 8). Data are expressed relative to β-actin (*P < 0.05; **P < 0.01 versus OVA+iBp; #P < 0.05; ##P < 0.01 versus normal).

Mentions: To investigate whether the elevation of PDE activity was due to enhanced PDE subtype gene expression, we measured the lung mRNA levels of subtypes PDE1 to PDE11 in control, OVA, OVA+iBp, and iBp rats. There were no significant changes in the expression of PDEs 1, 2, 9, 10, and 11 (data not shown). The expression of PDEs 3, 4A, 4D, 5, 7, and 8 was upregulated in OVA+iBp rats. Although PDE4A and PDE4D were significantly increased in the OVA+iBp rats, PDE4A did not increase in the OVA or iBprats, while simultaneous exposure to OVA and intramuscular iBpenhanced the PDE4A expression. Interestingly, the expression of PDE4D was significantly increased in iBprats, while in the OVA group it did not increase. This suggested that iBpspecially upregulated PDE4D (Figure 5). At the same time, this result also suggested that the increased cAMP-PDE activity might be due to enhanced PDE7 and PDE8 gene expression by simultaneous exposure to OVA and iBp, at least in part.


Effects of inactivated Bordetella pertussis on phosphodiesterase in the lung of ovalbumin sensitized and challenged rats.

Wang YJ, Song SD, Chen JC, Wang XF, Jiang YL, Xie QM, Chen JQ, Li ZG, Tang HF - Pulm Med (2014)

(a) Representative image of PDE subtype mRNA expression. (b) Levels of mRNA expression of lung PDE subtypes in normal (n = 8), OVA+iBp, iBp (n = 6), and OVA rats (n = 8). Data are expressed relative to β-actin (*P < 0.05; **P < 0.01 versus OVA+iBp; #P < 0.05; ##P < 0.01 versus normal).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4121004&req=5

fig5: (a) Representative image of PDE subtype mRNA expression. (b) Levels of mRNA expression of lung PDE subtypes in normal (n = 8), OVA+iBp, iBp (n = 6), and OVA rats (n = 8). Data are expressed relative to β-actin (*P < 0.05; **P < 0.01 versus OVA+iBp; #P < 0.05; ##P < 0.01 versus normal).
Mentions: To investigate whether the elevation of PDE activity was due to enhanced PDE subtype gene expression, we measured the lung mRNA levels of subtypes PDE1 to PDE11 in control, OVA, OVA+iBp, and iBp rats. There were no significant changes in the expression of PDEs 1, 2, 9, 10, and 11 (data not shown). The expression of PDEs 3, 4A, 4D, 5, 7, and 8 was upregulated in OVA+iBp rats. Although PDE4A and PDE4D were significantly increased in the OVA+iBp rats, PDE4A did not increase in the OVA or iBprats, while simultaneous exposure to OVA and intramuscular iBpenhanced the PDE4A expression. Interestingly, the expression of PDE4D was significantly increased in iBprats, while in the OVA group it did not increase. This suggested that iBpspecially upregulated PDE4D (Figure 5). At the same time, this result also suggested that the increased cAMP-PDE activity might be due to enhanced PDE7 and PDE8 gene expression by simultaneous exposure to OVA and iBp, at least in part.

Bottom Line: This paper indicated that inactivated Bordetella pertussis (iBp) can enhance the lung airway hyperreactivity of the rats sensitized and challenged with OVA.The mechanisms were involved in the upregulation of cAMP-PDE activity and PDE4A, PDE4D, and PDE3 gene expression in the lungs.So, our data suggested that cosensitization with OVA and iBp affects lung airway reactivity by modulating the lung cAMP-PDE activity and PDE4D gene expression.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Respiratory Drugs Research Laboratory of SFDA of China, School of Medicine, Zhejiang University, Hangzhou 310058, China ; Clinical College of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Hefei 230038, China.

ABSTRACT
This paper indicated that inactivated Bordetella pertussis (iBp) can enhance the lung airway hyperreactivity of the rats sensitized and challenged with OVA. The mechanisms were involved in the upregulation of cAMP-PDE activity and PDE4A, PDE4D, and PDE3 gene expression in the lungs. But only PDE4 activity was different between the OVA and OVA+iBp groups, and PDE4D expression was significantly increased in iBp rats alone. So, our data suggested that cosensitization with OVA and iBp affects lung airway reactivity by modulating the lung cAMP-PDE activity and PDE4D gene expression.

Show MeSH
Related in: MedlinePlus